skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1

Abstract

Recent studies have demonstrated that neuropilin 1 (NP-1) is involved in HTLV-1 entry; however, the role NP-1 plays in this process is not understood. We demonstrated that ectopic expression of human NP-1 but not NP-2 cDNA increased susceptibility to HTLV-1. SiRNA-mediated inhibition of NP-1 expression correlated with significant reduction of HTLV-1 Env-mediated fusion. The vascular endothelial growth factor (VEGF{sub 165}) caused downmodulation of surface NP-1 and inhibited HTLV-1 infection of U87 cells. In contrast, VEGF{sub 165} partially inhibited infection of primary astrocytes and had no significant effect on infection of HeLa cells. VEGF{sub 165} and antibodies to the glucose transporter protein 1 (anti-GLUT-1) were both needed to block infection of primary astrocytes, however, only anti-GLUT-1 antibodies were sufficient to block infection of HeLa cells. HTLV-1 Env forms complexes with both NP-1 and GLUT-1 in primary human astrocytes. The alternate usage of these two cellular receptors may have important implications regarding HTLV-1 neuro-tropism.

Authors:
 [1];  [2];  [3];  [1]
  1. Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Rm420, Indianapolis, IN (United States)
  2. Department of Biology, IUPUI School of Science (United States)
  3. Department of Medical and Molecular Genetics, Indiana University School of Medicine, 635 Barnhill Drive, Rm420, Indianapolis, IN (United States)
Publication Date:
OSTI Identifier:
21357582
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 396; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2009.10.015; PII: S0042-6822(09)00624-2; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; GLUCOSE; GLYCOPROTEINS; GROWTH FACTORS; HELA CELLS; INHIBITION; LEUKEMIA VIRUSES; RECEPTORS; ALDEHYDES; ANIMAL CELLS; CARBOHYDRATES; HEXOSES; MEMBRANE PROTEINS; MICROORGANISMS; MITOGENS; MONOSACCHARIDES; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; PARASITES; PROTEINS; SACCHARIDES; TUMOR CELLS; VIRUSES

Citation Formats

Qingwen, Jin, Alkhatib, Bashar, Cornetta, Kenneth, and Alkhatib, Ghalib. Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1. United States: N. p., 2010. Web.
Qingwen, Jin, Alkhatib, Bashar, Cornetta, Kenneth, & Alkhatib, Ghalib. Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1. United States.
Qingwen, Jin, Alkhatib, Bashar, Cornetta, Kenneth, and Alkhatib, Ghalib. 2010. "Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1". United States.
@article{osti_21357582,
title = {Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1},
author = {Qingwen, Jin and Alkhatib, Bashar and Cornetta, Kenneth and Alkhatib, Ghalib},
abstractNote = {Recent studies have demonstrated that neuropilin 1 (NP-1) is involved in HTLV-1 entry; however, the role NP-1 plays in this process is not understood. We demonstrated that ectopic expression of human NP-1 but not NP-2 cDNA increased susceptibility to HTLV-1. SiRNA-mediated inhibition of NP-1 expression correlated with significant reduction of HTLV-1 Env-mediated fusion. The vascular endothelial growth factor (VEGF{sub 165}) caused downmodulation of surface NP-1 and inhibited HTLV-1 infection of U87 cells. In contrast, VEGF{sub 165} partially inhibited infection of primary astrocytes and had no significant effect on infection of HeLa cells. VEGF{sub 165} and antibodies to the glucose transporter protein 1 (anti-GLUT-1) were both needed to block infection of primary astrocytes, however, only anti-GLUT-1 antibodies were sufficient to block infection of HeLa cells. HTLV-1 Env forms complexes with both NP-1 and GLUT-1 in primary human astrocytes. The alternate usage of these two cellular receptors may have important implications regarding HTLV-1 neuro-tropism.},
doi = {},
url = {https://www.osti.gov/biblio/21357582}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 396,
place = {United States},
year = {Wed Jan 20 00:00:00 EST 2010},
month = {Wed Jan 20 00:00:00 EST 2010}
}