Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1
Abstract
Recent studies have demonstrated that neuropilin 1 (NP-1) is involved in HTLV-1 entry; however, the role NP-1 plays in this process is not understood. We demonstrated that ectopic expression of human NP-1 but not NP-2 cDNA increased susceptibility to HTLV-1. SiRNA-mediated inhibition of NP-1 expression correlated with significant reduction of HTLV-1 Env-mediated fusion. The vascular endothelial growth factor (VEGF{sub 165}) caused downmodulation of surface NP-1 and inhibited HTLV-1 infection of U87 cells. In contrast, VEGF{sub 165} partially inhibited infection of primary astrocytes and had no significant effect on infection of HeLa cells. VEGF{sub 165} and antibodies to the glucose transporter protein 1 (anti-GLUT-1) were both needed to block infection of primary astrocytes, however, only anti-GLUT-1 antibodies were sufficient to block infection of HeLa cells. HTLV-1 Env forms complexes with both NP-1 and GLUT-1 in primary human astrocytes. The alternate usage of these two cellular receptors may have important implications regarding HTLV-1 neuro-tropism.
- Authors:
-
- Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Rm420, Indianapolis, IN (United States)
- Department of Biology, IUPUI School of Science (United States)
- Department of Medical and Molecular Genetics, Indiana University School of Medicine, 635 Barnhill Drive, Rm420, Indianapolis, IN (United States)
- Publication Date:
- OSTI Identifier:
- 21357582
- Resource Type:
- Journal Article
- Journal Name:
- Virology
- Additional Journal Information:
- Journal Volume: 396; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2009.10.015; PII: S0042-6822(09)00624-2; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANTIBODIES; GLUCOSE; GLYCOPROTEINS; GROWTH FACTORS; HELA CELLS; INHIBITION; LEUKEMIA VIRUSES; RECEPTORS; ALDEHYDES; ANIMAL CELLS; CARBOHYDRATES; HEXOSES; MEMBRANE PROTEINS; MICROORGANISMS; MITOGENS; MONOSACCHARIDES; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; PARASITES; PROTEINS; SACCHARIDES; TUMOR CELLS; VIRUSES
Citation Formats
Qingwen, Jin, Alkhatib, Bashar, Cornetta, Kenneth, and Alkhatib, Ghalib. Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1. United States: N. p., 2010.
Web.
Qingwen, Jin, Alkhatib, Bashar, Cornetta, Kenneth, & Alkhatib, Ghalib. Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1. United States.
Qingwen, Jin, Alkhatib, Bashar, Cornetta, Kenneth, and Alkhatib, Ghalib. 2010.
"Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1". United States.
@article{osti_21357582,
title = {Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1},
author = {Qingwen, Jin and Alkhatib, Bashar and Cornetta, Kenneth and Alkhatib, Ghalib},
abstractNote = {Recent studies have demonstrated that neuropilin 1 (NP-1) is involved in HTLV-1 entry; however, the role NP-1 plays in this process is not understood. We demonstrated that ectopic expression of human NP-1 but not NP-2 cDNA increased susceptibility to HTLV-1. SiRNA-mediated inhibition of NP-1 expression correlated with significant reduction of HTLV-1 Env-mediated fusion. The vascular endothelial growth factor (VEGF{sub 165}) caused downmodulation of surface NP-1 and inhibited HTLV-1 infection of U87 cells. In contrast, VEGF{sub 165} partially inhibited infection of primary astrocytes and had no significant effect on infection of HeLa cells. VEGF{sub 165} and antibodies to the glucose transporter protein 1 (anti-GLUT-1) were both needed to block infection of primary astrocytes, however, only anti-GLUT-1 antibodies were sufficient to block infection of HeLa cells. HTLV-1 Env forms complexes with both NP-1 and GLUT-1 in primary human astrocytes. The alternate usage of these two cellular receptors may have important implications regarding HTLV-1 neuro-tropism.},
doi = {},
url = {https://www.osti.gov/biblio/21357582},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 396,
place = {United States},
year = {Wed Jan 20 00:00:00 EST 2010},
month = {Wed Jan 20 00:00:00 EST 2010}
}