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Title: Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes

Abstract

Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.

Authors:
; ; ; ; ;  [1];  [2];  [1];  [3];  [1];  [4];  [1]
  1. Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550 (Japan)
  2. The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550 (Japan)
  3. The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550 (Japan)
  4. Department of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555 (Japan)
Publication Date:
OSTI Identifier:
21357496
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 386; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2009.01.039; PII: S0042-6822(09)00038-5; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; ARABIDOPSIS; GENES; MERISTEMS; PHENOTYPE; PLANT GROWTH; TOBACCO; TOMATOES; VIRUSES; FOOD; FRUITS; GROWTH; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MICROORGANISMS; PARASITES; PLANT TISSUES; PLANTS

Citation Formats

Igarashi, Aki, Yamagata, Kousuke, Sugai, Tomokazu, Takahashi, Yukari, Sugawara, Emiko, Tamura, Akihiro, Yaegashi, Hajime, Yamagishi, Noriko, Takahashi, Tsubasa, Isogai, Masamichi, Takahashi, Hideki, Yoshikawa, Nobuyuki, The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, and The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550. Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes. United States: N. p., 2009. Web. doi:10.1016/j.virol.2009.01.039.
Igarashi, Aki, Yamagata, Kousuke, Sugai, Tomokazu, Takahashi, Yukari, Sugawara, Emiko, Tamura, Akihiro, Yaegashi, Hajime, Yamagishi, Noriko, Takahashi, Tsubasa, Isogai, Masamichi, Takahashi, Hideki, Yoshikawa, Nobuyuki, The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, & The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550. Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes. United States. https://doi.org/10.1016/j.virol.2009.01.039
Igarashi, Aki, Yamagata, Kousuke, Sugai, Tomokazu, Takahashi, Yukari, Sugawara, Emiko, Tamura, Akihiro, Yaegashi, Hajime, Yamagishi, Noriko, Takahashi, Tsubasa, Isogai, Masamichi, Takahashi, Hideki, Yoshikawa, Nobuyuki, The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, and The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550. 2009. "Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes". United States. https://doi.org/10.1016/j.virol.2009.01.039.
@article{osti_21357496,
title = {Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes},
author = {Igarashi, Aki and Yamagata, Kousuke and Sugai, Tomokazu and Takahashi, Yukari and Sugawara, Emiko and Tamura, Akihiro and Yaegashi, Hajime and Yamagishi, Noriko and Takahashi, Tsubasa and Isogai, Masamichi and Takahashi, Hideki and Yoshikawa, Nobuyuki and The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550 and The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550},
abstractNote = {Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.},
doi = {10.1016/j.virol.2009.01.039},
url = {https://www.osti.gov/biblio/21357496}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 386,
place = {United States},
year = {Fri Apr 10 00:00:00 EDT 2009},
month = {Fri Apr 10 00:00:00 EDT 2009}
}