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Title: Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells

Abstract

Butyl benzyl phthalate (BBP), an endocrine disruptor present in the environment, exerts its genomic effects via intracellular steroid receptors and elicits non-genomic effects by interfering with membrane ion-channel receptors. We previously found that BBP blocks the calcium signaling coupled with P2X receptors in PC12 cells (Liu and Chen, 2006). Osteoblast P2X receptors were recently reported to play a role in cell proliferation and bone remodeling. In this present study, the effects of BBP on ATP-induced responses were investigated in human osteosarcoma HOS cells. These receptors mRNA had been detected, named P2X4, P2X7, P2Y2, P2Y4, P2Y5, P2Y9, and P2Y11, in human osteosarcoma HOS cells by RT-PCR. The enhancement of cell proliferation and the decrease of cytoviability had both been shown to be coupled to stimulation via different concentrations of ATP. BBP suppressed the ATP-induced calcium influx (mainly coupled with P2X) and cell proliferation but not the ATP-induced intracellular calcium release (mainly coupled with P2Y) and cytotoxicity in human osteosarcoma HOS cells. Suramin, a common P2 receptor's antagonist, blocked the ATP-induced calcium signaling, cell proliferation, and cytotoxicity. We suggest that P2X is mainly responsible for cell proliferation, and P2Y might be partially responsible for the observed cytotoxicity. BBP suppressed the calcium signalingmore » coupled with P2X, suppressing cell proliferation. Since the importance of P2X receptors during bone metastasis has recently become apparent, the possible toxic risk of environmental BBP during bone remodeling is a public problem of concern.« less

Authors:
 [1];  [1]
  1. Department of Microbiology, Soochow University, Shihlin, Taipei, Taiwan (China)
Publication Date:
OSTI Identifier:
21344933
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 244; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2010.01.007; PII: S0041-008X(10)00018-9; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ATP; CALCIUM; CELL PROLIFERATION; CONNECTIVE TISSUE CELLS; HAZARDS; MEN; MESSENGER-RNA; METASTASES; OSTEOSARCOMAS; PHTHALATES; RECEPTORS; SKELETON; STEROIDS; STIMULATION; TOXICITY; ALKALINE EARTH METALS; ANIMAL CELLS; ANIMALS; BODY; CARBOXYLIC ACID SALTS; DISEASES; ELEMENTS; MALES; MAMMALS; MAN; MEMBRANE PROTEINS; METALS; NEOPLASMS; NUCLEIC ACIDS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; PRIMATES; PROTEINS; RNA; SARCOMAS; SKELETAL DISEASES; SOMATIC CELLS; VERTEBRATES

Citation Formats

Liu, P.-S., E-mail: pslediting@mail.scu.edu.t, and Chen, C.-Y. Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells. United States: N. p., 2010. Web. doi:10.1016/j.taap.2010.01.007.
Liu, P.-S., E-mail: pslediting@mail.scu.edu.t, & Chen, C.-Y. Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells. United States. doi:10.1016/j.taap.2010.01.007.
Liu, P.-S., E-mail: pslediting@mail.scu.edu.t, and Chen, C.-Y. Sat . "Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells". United States. doi:10.1016/j.taap.2010.01.007.
@article{osti_21344933,
title = {Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells},
author = {Liu, P.-S., E-mail: pslediting@mail.scu.edu.t and Chen, C.-Y.},
abstractNote = {Butyl benzyl phthalate (BBP), an endocrine disruptor present in the environment, exerts its genomic effects via intracellular steroid receptors and elicits non-genomic effects by interfering with membrane ion-channel receptors. We previously found that BBP blocks the calcium signaling coupled with P2X receptors in PC12 cells (Liu and Chen, 2006). Osteoblast P2X receptors were recently reported to play a role in cell proliferation and bone remodeling. In this present study, the effects of BBP on ATP-induced responses were investigated in human osteosarcoma HOS cells. These receptors mRNA had been detected, named P2X4, P2X7, P2Y2, P2Y4, P2Y5, P2Y9, and P2Y11, in human osteosarcoma HOS cells by RT-PCR. The enhancement of cell proliferation and the decrease of cytoviability had both been shown to be coupled to stimulation via different concentrations of ATP. BBP suppressed the ATP-induced calcium influx (mainly coupled with P2X) and cell proliferation but not the ATP-induced intracellular calcium release (mainly coupled with P2Y) and cytotoxicity in human osteosarcoma HOS cells. Suramin, a common P2 receptor's antagonist, blocked the ATP-induced calcium signaling, cell proliferation, and cytotoxicity. We suggest that P2X is mainly responsible for cell proliferation, and P2Y might be partially responsible for the observed cytotoxicity. BBP suppressed the calcium signaling coupled with P2X, suppressing cell proliferation. Since the importance of P2X receptors during bone metastasis has recently become apparent, the possible toxic risk of environmental BBP during bone remodeling is a public problem of concern.},
doi = {10.1016/j.taap.2010.01.007},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 244,
place = {United States},
year = {2010},
month = {5}
}