In utero and in vitro effects of benzene and its metabolites on erythroid differentiation and the role of reactive oxygen species

Journal Article · Sat May 01 00:00:00 EDT 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2010.01.002· OSTI ID:21344929

Badham, Helen J ^[1]

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1. Department of Pharmacology and Toxicology. Queen's University, Kingston, Ontario, K7L 3N6 (Canada)

Benzene is a ubiquitous occupational and environmental toxicant. Exposures to benzene both prenatally and during adulthood are associated with the development of disorders such as aplastic anemia and leukemia. Mechanisms of benzene toxicity are unknown; however, generation of reactive oxygen species (ROS) by benzene metabolites may play a role. Little is known regarding the effects of benzene metabolites on erythropoiesis. Therefore, to determine the effects of in utero exposure to benzene on the growth and differentiation of fetal erythroid progenitor cells (CFU-E), pregnant CD-1 mice were exposed to benzene and CFU-E numbers were assessed in fetal liver (hematopoietic) tissue. In addition, to determine the effect of benzene metabolite-induced ROS generation on erythropoiesis, HD3 chicken erythroblast cells were exposed to benzene, phenol, or hydroquinone followed by stimulation of erythrocyte differentiation. Our results show that in utero exposure to benzene caused significant alterations in female offspring CFU-E numbers. In addition, exposure to hydroquinone, but not benzene or phenol, significantly reduced the percentage of differentiated HD3 cells, which was associated with an increase in ROS. Pretreatment of HD3 cells with polyethylene glycol-conjugated superoxide dismutase (PEG-SOD) prevented hydroquinone-induced inhibition of erythropoiesis, supporting the hypothesis that ROS generation is involved in the development of benzene erythrotoxicity. In conclusion, this study provided evidence that ROS generated as a result of benzene metabolism may significantly alter erythroid differentiation, potentially leading to the development of Blood Disorders.

• OSTI ID: 21344929
• Journal Information: Toxicology and Applied Pharmacology, Vol. 244, Issue 3; Other Information: DOI: 10.1016/j.taap.2010.01.002; PII: S0041-008X(10)00013-X; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANEMIAS
ANIMAL GROWTH
BENZENE
BONE MARROW CELLS
CHICKENS
ERYTHROCYTES
ERYTHROPOIESIS
IN VITRO
INHIBITION
LEUKEMIA
LIVER
METABOLISM
METABOLITES
MICE
OXYGEN
PHENOL
POLYETHYLENE GLYCOLS
STIMULATION
SUPEROXIDE DISMUTASE
TOXICITY
UTERUS
ALCOHOLS
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASES
ELEMENTS
ENZYMES
FEMALE GENITALS
FOWL
GLANDS
GLYCOLS
GROWTH
HEMIC DISEASES
HYDROCARBONS
HYDROXY COMPOUNDS
IMMUNE SYSTEM DISEASES
MAMMALS
MATERIALS
NEOPLASMS
NONMETALS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
OXIDOREDUCTASES
PHENOLS
POLYMERS
PROTEINS
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES