Immobilization of Proteins on a Glass Surface at High Density

Journal Article · Mon Jul 06 00:00:00 EDT 2009 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3183513· OSTI ID:21316839

Thomas, Marlon; Vullev, Valentine I ^[1]; Jiandi, Wan ^[1]

---

1. Department of Bioengineering, University of California, Riverside, CA 92507 (United States)

We describe a rational molecular-level design of biocompatible surface coatings and immobilization of biological species onto them to produce biofunctional interfaces. Our method adapted a strategy for coating glass and other silica-type substrates with bioinert layers of polyethylene glycol (PEG). The introduction of {alpha}, {omega}-bifunctional polymers into the coatings allowed for covalent attachment of proteins to the PEGylated surfaces. Spectroscopic studies indicate that the surface-bound proteins had their biological activity preserved.

• OSTI ID: 21316839
• Journal Information: AIP Conference Proceedings, Vol. 1140, Issue 1; Conference: 2008 joint annual conference of the National Society of Black Physicists and the National Society of Hispanic Physicists; 35. annual day of scientific lectures; 31. annual meeting, Washington, DC (United States), 20-23 Feb 2008; Other Information: DOI: 10.1063/1.3183513; (c) 2009 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
BIOLOGICAL MATERIALS
COVALENCE
DENSITY
FLUORESCENCE
GLASS
INTERFACES
LAYERS
PHOTOELECTRON SPECTROSCOPY
POLYETHYLENE GLYCOLS
PROTECTIVE COATINGS
PROTEINS
SILICA
SUBSTRATES
SURFACES