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Title: Studies on Pentoxifylline and Tocopherol Combination for Radiation-Induced Heart Disease in Rats

Abstract

Purpose: To investigate whether the application of pentoxifylline (PTX) and tocopherol l (Vit. E) could modify the development of radiation-induced heart disease and downregulate the expression of transforming growth factor (TGF)-{beta}1mRNA in rats. Methods and Materials: A total of 120 Sprague-Dawley rats were separated into four groups: control group, irradiated group, experimental group 1, and experiment group 2. Supplementation was started 3 days before irradiation; in experimental group 1, injection of PTX (15 mg/kg/d) and Vit. E (5.5 mg/kg/d) continued till the 12th week postirradiation, whereas in experimental group 2 it was continued until the 24th week postirradiation. All rats were administrated a single dose of 20 Gy irradiation to the heart except the control group. Histopathologic evaluation was performed at various time points (Days 1, 2, 4, 8, and 12 and 24th week) up to 24 weeks after irradiation. Changes of levels of TGF-{beta}1 mRNA expression were also investigated at the same time points using competitive polymerase chain reaction. Results: Compared with the irradiated group, levels of TGF-{beta}1 mRNA of the rat hearts were relatively low in the two experimental groups on the 12th week postirradiation. In experimental group 1, there was a rebound expression of TGF-{beta}1 mRNA onmore » the 24th week postirradiation, whereas that of the experimental group 2 remained low (p < 0.05). The proportions of collagen fibers of the two experimental groups were lower than that of irradiated group (p < 0.05). A rebound could be observed in the experimental group 1. Conclusion: PTX and Vit. E downregulated the expression of TGF-{beta}1 mRNA. The irradiated rat hearts showed a marked pathologic response to the drugs. The withdrawal of drugs in the 12th week postirradiation could cause rebound effects of the development of fibrosis.« less

Authors:
 [1];  [2];  [3]; ; ;  [1];  [2];  [1];  [2];  [1];  [2];  [1];  [4]
  1. State Key Laboratory of Oncology in South China, Guangzhou, Guangdong (China)
  2. (China)
  3. Department of Cardiac Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong (China)
  4. (China), E-mail: esophagus2003@yahoo.com.cn
Publication Date:
OSTI Identifier:
21281968
Resource Type:
Journal Article
Journal Name:
International Journal of Radiation Oncology, Biology and Physics
Additional Journal Information:
Journal Volume: 73; Journal Issue: 5; Other Information: DOI: 10.1016/j.ijrobp.2008.12.005; PII: S0360-3016(08)03861-3; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0360-3016
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARDIOVASCULAR DISEASES; COLLAGEN; DRUGS; FIBROSIS; GROWTH FACTORS; HEART; IRRADIATION; POLYMERASE CHAIN REACTION; RADIATION DOSES; RATS; VITAMIN E

Citation Formats

Liu Hui, Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Xiong Mai, Xia Yunfei, Cui Nianji, Lu Rubiao, Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Deng Ling, Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Lin Yuehao, Clinical Laboratory, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Rong Tiehua, and Department of Thoracic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong. Studies on Pentoxifylline and Tocopherol Combination for Radiation-Induced Heart Disease in Rats. United States: N. p., 2009. Web. doi:10.1016/j.ijrobp.2008.12.005.
Liu Hui, Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Xiong Mai, Xia Yunfei, Cui Nianji, Lu Rubiao, Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Deng Ling, Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Lin Yuehao, Clinical Laboratory, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Rong Tiehua, & Department of Thoracic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong. Studies on Pentoxifylline and Tocopherol Combination for Radiation-Induced Heart Disease in Rats. United States. doi:10.1016/j.ijrobp.2008.12.005.
Liu Hui, Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Xiong Mai, Xia Yunfei, Cui Nianji, Lu Rubiao, Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Deng Ling, Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Lin Yuehao, Clinical Laboratory, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, Rong Tiehua, and Department of Thoracic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong. Wed . "Studies on Pentoxifylline and Tocopherol Combination for Radiation-Induced Heart Disease in Rats". United States. doi:10.1016/j.ijrobp.2008.12.005.
@article{osti_21281968,
title = {Studies on Pentoxifylline and Tocopherol Combination for Radiation-Induced Heart Disease in Rats},
author = {Liu Hui and Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong and Xiong Mai and Xia Yunfei and Cui Nianji and Lu Rubiao and Department of Radiotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong and Deng Ling and Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong and Lin Yuehao and Clinical Laboratory, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong and Rong Tiehua and Department of Thoracic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong},
abstractNote = {Purpose: To investigate whether the application of pentoxifylline (PTX) and tocopherol l (Vit. E) could modify the development of radiation-induced heart disease and downregulate the expression of transforming growth factor (TGF)-{beta}1mRNA in rats. Methods and Materials: A total of 120 Sprague-Dawley rats were separated into four groups: control group, irradiated group, experimental group 1, and experiment group 2. Supplementation was started 3 days before irradiation; in experimental group 1, injection of PTX (15 mg/kg/d) and Vit. E (5.5 mg/kg/d) continued till the 12th week postirradiation, whereas in experimental group 2 it was continued until the 24th week postirradiation. All rats were administrated a single dose of 20 Gy irradiation to the heart except the control group. Histopathologic evaluation was performed at various time points (Days 1, 2, 4, 8, and 12 and 24th week) up to 24 weeks after irradiation. Changes of levels of TGF-{beta}1 mRNA expression were also investigated at the same time points using competitive polymerase chain reaction. Results: Compared with the irradiated group, levels of TGF-{beta}1 mRNA of the rat hearts were relatively low in the two experimental groups on the 12th week postirradiation. In experimental group 1, there was a rebound expression of TGF-{beta}1 mRNA on the 24th week postirradiation, whereas that of the experimental group 2 remained low (p < 0.05). The proportions of collagen fibers of the two experimental groups were lower than that of irradiated group (p < 0.05). A rebound could be observed in the experimental group 1. Conclusion: PTX and Vit. E downregulated the expression of TGF-{beta}1 mRNA. The irradiated rat hearts showed a marked pathologic response to the drugs. The withdrawal of drugs in the 12th week postirradiation could cause rebound effects of the development of fibrosis.},
doi = {10.1016/j.ijrobp.2008.12.005},
journal = {International Journal of Radiation Oncology, Biology and Physics},
issn = {0360-3016},
number = 5,
volume = 73,
place = {United States},
year = {2009},
month = {4}
}