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Title: Pulmonary toxicity and kinetic study of Cy5.5-conjugated superparamagnetic iron oxide nanoparticles by optical imaging

Abstract

Recent advances in the development of nanotechnology and devices now make it possible to accurately deliver drugs or genes to the lung. Magnetic nanoparticles can be used as contrast agents, thermal therapy for cancer, and be made to concentrate to target sites through an external magnetic field. However, these advantages may also become problematic when taking into account safety and toxicological factors. This study demonstrated the pulmonary toxicity and kinetic profile of anti-biofouling polymer coated, Cy5.5-conjugated thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) by optical imaging. Negatively charged, 36 nm-sized, Cy5.5-conjugated TCL-SPION was prepared for optical imaging probe. Cy5.5-conjugated TCL-SPION was intratracheally instilled into the lung by a non-surgical method. Cy5.5-conjugated TCL-SPION slightly induced pulmonary inflammation. The instilled nanoparticles were distributed mainly in the lung and excreted in the urine via glomerular filtration. Urinary excretion was peaked at 3 h after instillation. No toxicity was found under the concentration of 1.8 mg/kg and the half-lives of nanoparticles in the lung and urine were estimated to be about 14.4 {+-} 0.54 h and 24.7 {+-} 1.02 h, respectively. Although further studies are required, our results showed that Cy5.5-conjugated TCL-SPION can be a good candidate for use in pulmonary delivery vehicles andmore » diagnostic probes.« less

Authors:
 [1]; ; ; ; ;  [1];  [2]; ;  [3];  [1]
  1. Division of Toxicologic Pathology, Department of Toxicological Research, National Institute of Toxicological Research, Korea Food and Drug Administration, 231 Jinhoungno Eunpyung-ku, Seoul 122-704 (Korea, Republic of)
  2. Division of Genetic Toxicology, Department of Toxicological Research, National Institute of Toxicological Research, Korea Food and Drug Administration, 231 Jinhoungno Eunpyung-ku, Seoul 122-704 (Korea, Republic of)
  3. Research Center for Biomolecular Nanotechnology, Department of Life Science, Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Buk-gu, Gwangju 500-712 (Korea, Republic of)
Publication Date:
OSTI Identifier:
21272633
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 239; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2009.05.026; PII: S0041-008X(09)00226-9; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL FOULING; DELIVERY; DRUGS; EXCRETION; FILTRATION; HALF-LIFE; INFLAMMATION; IRON OXIDES; LUNGS; MAGNETIC FIELDS; NANOSTRUCTURES; NEOPLASMS; PARTICLES; POLYMERS; SUPERPARAMAGNETISM; SURGERY; THERAPY; TOXICITY; TRANSLOCATION; URINE

Citation Formats

Cho, Wan-Seob, Respiratory Medicine Unit, ELEGI/Colt Laboratory, Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, Cho, Minjung, Kim, Seoung Ryul, Choi, Mina, Lee, Jeong Yeon, Han, Beom Seok, Park, Sue Nie, Yu, Mi Kyung, Jon, Sangyong, and Jeong, Jayoung. Pulmonary toxicity and kinetic study of Cy5.5-conjugated superparamagnetic iron oxide nanoparticles by optical imaging. United States: N. p., 2009. Web. doi:10.1016/j.taap.2009.05.026.
Cho, Wan-Seob, Respiratory Medicine Unit, ELEGI/Colt Laboratory, Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, Cho, Minjung, Kim, Seoung Ryul, Choi, Mina, Lee, Jeong Yeon, Han, Beom Seok, Park, Sue Nie, Yu, Mi Kyung, Jon, Sangyong, & Jeong, Jayoung. Pulmonary toxicity and kinetic study of Cy5.5-conjugated superparamagnetic iron oxide nanoparticles by optical imaging. United States. https://doi.org/10.1016/j.taap.2009.05.026
Cho, Wan-Seob, Respiratory Medicine Unit, ELEGI/Colt Laboratory, Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, Cho, Minjung, Kim, Seoung Ryul, Choi, Mina, Lee, Jeong Yeon, Han, Beom Seok, Park, Sue Nie, Yu, Mi Kyung, Jon, Sangyong, and Jeong, Jayoung. Sat . "Pulmonary toxicity and kinetic study of Cy5.5-conjugated superparamagnetic iron oxide nanoparticles by optical imaging". United States. https://doi.org/10.1016/j.taap.2009.05.026.
@article{osti_21272633,
title = {Pulmonary toxicity and kinetic study of Cy5.5-conjugated superparamagnetic iron oxide nanoparticles by optical imaging},
author = {Cho, Wan-Seob and Respiratory Medicine Unit, ELEGI/Colt Laboratory, Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ and Cho, Minjung and Kim, Seoung Ryul and Choi, Mina and Lee, Jeong Yeon and Han, Beom Seok and Park, Sue Nie and Yu, Mi Kyung and Jon, Sangyong and Jeong, Jayoung},
abstractNote = {Recent advances in the development of nanotechnology and devices now make it possible to accurately deliver drugs or genes to the lung. Magnetic nanoparticles can be used as contrast agents, thermal therapy for cancer, and be made to concentrate to target sites through an external magnetic field. However, these advantages may also become problematic when taking into account safety and toxicological factors. This study demonstrated the pulmonary toxicity and kinetic profile of anti-biofouling polymer coated, Cy5.5-conjugated thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) by optical imaging. Negatively charged, 36 nm-sized, Cy5.5-conjugated TCL-SPION was prepared for optical imaging probe. Cy5.5-conjugated TCL-SPION was intratracheally instilled into the lung by a non-surgical method. Cy5.5-conjugated TCL-SPION slightly induced pulmonary inflammation. The instilled nanoparticles were distributed mainly in the lung and excreted in the urine via glomerular filtration. Urinary excretion was peaked at 3 h after instillation. No toxicity was found under the concentration of 1.8 mg/kg and the half-lives of nanoparticles in the lung and urine were estimated to be about 14.4 {+-} 0.54 h and 24.7 {+-} 1.02 h, respectively. Although further studies are required, our results showed that Cy5.5-conjugated TCL-SPION can be a good candidate for use in pulmonary delivery vehicles and diagnostic probes.},
doi = {10.1016/j.taap.2009.05.026},
url = {https://www.osti.gov/biblio/21272633}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 239,
place = {United States},
year = {2009},
month = {8}
}