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Title: Cloning and characterization of two flavohemoglobins from Aspergillus oryzae

Abstract

Two flavohemoglobin (FHb) genes, fhb1 and fhb2, were cloned from Aspergillus oryzae. The amino acid sequences of the deduced FHb1 and FHb2 showed high identity to other FHbs except for the predicted mitochondrial targeting signal in the N-terminus of FHb2. The recombinant proteins displayed absorption spectra similar to those of other FHbs. FHb1 and FHb2 were estimated to be a monomer and a dimer in solution, respectively. Both of the isozymes exhibit high NO dioxygenase (NOD) activity. FHb1 utilizes either NADH or NADPH as an electron donor, whereas FHb2 can only use NADH. These results suggest that FHb1 and FHb2 are fungal counterparts of bacterial FHbs and act as NO detoxification enzymes in the cytosol and mitochondria, respectively. This study is the first to show that a microorganism contains two isozymes of FHb and that intracellular localization of the isozymes could differ.

Authors:
; ; ; ;  [1];  [2]
  1. Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)
  2. Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan), E-mail: ahshoun@mail.ecc.u-tokyo.ac.jp
Publication Date:
OSTI Identifier:
21255943
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 381; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2009.01.112; PII: S0006-291X(09)00159-4; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSORPTION SPECTRA; AMINO ACID SEQUENCE; ASPERGILLUS; BINDING ENERGY; CLONING; DETOXIFICATION; ENZYMES; GENES; HEME; MICROORGANISMS; MITOCHONDRIA; NITRIC OXIDE; SIGNALS

Citation Formats

Zhou Shengmin, Fushinobu, Shinya, Nakanishi, Yoshito, Kim, Sang-Wan, Wakagi, Takayoshi, and Shoun, Hirofumi. Cloning and characterization of two flavohemoglobins from Aspergillus oryzae. United States: N. p., 2009. Web. doi:10.1016/j.bbrc.2009.01.112.
Zhou Shengmin, Fushinobu, Shinya, Nakanishi, Yoshito, Kim, Sang-Wan, Wakagi, Takayoshi, & Shoun, Hirofumi. Cloning and characterization of two flavohemoglobins from Aspergillus oryzae. United States. doi:10.1016/j.bbrc.2009.01.112.
Zhou Shengmin, Fushinobu, Shinya, Nakanishi, Yoshito, Kim, Sang-Wan, Wakagi, Takayoshi, and Shoun, Hirofumi. Fri . "Cloning and characterization of two flavohemoglobins from Aspergillus oryzae". United States. doi:10.1016/j.bbrc.2009.01.112.
@article{osti_21255943,
title = {Cloning and characterization of two flavohemoglobins from Aspergillus oryzae},
author = {Zhou Shengmin and Fushinobu, Shinya and Nakanishi, Yoshito and Kim, Sang-Wan and Wakagi, Takayoshi and Shoun, Hirofumi},
abstractNote = {Two flavohemoglobin (FHb) genes, fhb1 and fhb2, were cloned from Aspergillus oryzae. The amino acid sequences of the deduced FHb1 and FHb2 showed high identity to other FHbs except for the predicted mitochondrial targeting signal in the N-terminus of FHb2. The recombinant proteins displayed absorption spectra similar to those of other FHbs. FHb1 and FHb2 were estimated to be a monomer and a dimer in solution, respectively. Both of the isozymes exhibit high NO dioxygenase (NOD) activity. FHb1 utilizes either NADH or NADPH as an electron donor, whereas FHb2 can only use NADH. These results suggest that FHb1 and FHb2 are fungal counterparts of bacterial FHbs and act as NO detoxification enzymes in the cytosol and mitochondria, respectively. This study is the first to show that a microorganism contains two isozymes of FHb and that intracellular localization of the isozymes could differ.},
doi = {10.1016/j.bbrc.2009.01.112},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 381,
place = {United States},
year = {Fri Mar 27 00:00:00 EDT 2009},
month = {Fri Mar 27 00:00:00 EDT 2009}
}