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Title: Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion

Abstract

Integrins are transmembrane receptors for cell adhesion to the extracellular matrix. In cell migration, integrins are endocytosed from the plasma membrane or the cell surface, transported in vesicles and exocytosed actively at the cell front. In the present study, we examined the roles of VAMP3, a SNARE protein that mediates exocytosis, in cell migration and integrin trafficking. Small interfering RNA (siRNA)-induced silencing of VAMP3 inhibited chemotactic cell migration by more than 60% without affecting cell proliferation. VAMP3 silencing reduced the levels of {beta}1 integrin at the cell surface but had no effect on total cellular {beta}1 integrin, indicating that VAMP3 is required for trafficking of {beta}1 integrin to the plasma membrane. Furthermore, VAMP3 silencing diminished cell adhesion to laminin but not to fibronectin or collagen. Taken together, these data suggest that VAMP3-dependent integrin trafficking is crucial in cell migration and cell adhesion to laminin.

Authors:
; ; ;  [1];  [2]
  1. Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, 319 Abraham Flexner Way, Room 515, Louisville, KY 40202 (United States)
  2. Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, 319 Abraham Flexner Way, Room 515, Louisville, KY 40202 (United States), E-mail: chuan.hu@louisville.edu
Publication Date:
OSTI Identifier:
21255906
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 380; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2009.01.036; PII: S0006-291X(09)00054-0; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADHESION; CELL PROLIFERATION; COLLAGEN; MEMBRANES; RECEPTORS; RNA; SURFACES

Citation Formats

Luftman, Kevin, Hasan, Nazarul, Day, Paul, Hardee, Deborah, and Hu Chuan. Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion. United States: N. p., 2009. Web. doi:10.1016/j.bbrc.2009.01.036.
Luftman, Kevin, Hasan, Nazarul, Day, Paul, Hardee, Deborah, & Hu Chuan. Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion. United States. doi:10.1016/j.bbrc.2009.01.036.
Luftman, Kevin, Hasan, Nazarul, Day, Paul, Hardee, Deborah, and Hu Chuan. Fri . "Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion". United States. doi:10.1016/j.bbrc.2009.01.036.
@article{osti_21255906,
title = {Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion},
author = {Luftman, Kevin and Hasan, Nazarul and Day, Paul and Hardee, Deborah and Hu Chuan},
abstractNote = {Integrins are transmembrane receptors for cell adhesion to the extracellular matrix. In cell migration, integrins are endocytosed from the plasma membrane or the cell surface, transported in vesicles and exocytosed actively at the cell front. In the present study, we examined the roles of VAMP3, a SNARE protein that mediates exocytosis, in cell migration and integrin trafficking. Small interfering RNA (siRNA)-induced silencing of VAMP3 inhibited chemotactic cell migration by more than 60% without affecting cell proliferation. VAMP3 silencing reduced the levels of {beta}1 integrin at the cell surface but had no effect on total cellular {beta}1 integrin, indicating that VAMP3 is required for trafficking of {beta}1 integrin to the plasma membrane. Furthermore, VAMP3 silencing diminished cell adhesion to laminin but not to fibronectin or collagen. Taken together, these data suggest that VAMP3-dependent integrin trafficking is crucial in cell migration and cell adhesion to laminin.},
doi = {10.1016/j.bbrc.2009.01.036},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 380,
place = {United States},
year = {Fri Feb 27 00:00:00 EST 2009},
month = {Fri Feb 27 00:00:00 EST 2009}
}
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