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Title: Development of a STAT3 reporter prostate cancer cell line for high throughput screening of STAT3 activators and inhibitors

Abstract

STAT3 is constitutively activated in several cancers, including prostate cancer, and is therefore, a potential target for cancer therapy. DU-145 prostate cancer cells were stably co-transfected with STAT3 reporter and puromycin resistant plasmids to create a stable STAT3 reporter cell line that can be used for high throughput screening of STAT3 modulators. The applicability of this cell line was tested with two known activators and inhibitors of STAT3. As expected, EGF and IL-6 increased STAT3 reporter activity and enhanced the nuclear localization of phosphorylated STAT3 (pSTAT3); whereas Cucurbitacin I and AG490 decreased STAT3 reporter activity dose and time-dependently and reduced the localization of pSTAT3 in the nuclei of prostate cancer cells. Given the importance of STAT3 in cancer initiation and progression, the development of a stable STAT3 reporter cell line in prostate cancer cells provides a rapid, sensitive, and cost effective method for the screening of potential STAT3 modulators.

Authors:
 [1];  [2]
  1. Department of Biochemistry, Molecular and Cellular Biology, Georgetown University Medical Center, Medical-Dental Building, Room C406B, 3900 Reservoir Road, Northwest, Washington, DC 20057 (United States)
  2. Department of Biochemistry, Molecular and Cellular Biology, Georgetown University Medical Center, Medical-Dental Building, Room C406B, 3900 Reservoir Road, Northwest, Washington, DC 20057 (United States), E-mail: ppb@georgetown.edu
Publication Date:
OSTI Identifier:
21255787
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 377; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2008.10.025; PII: S0006-291X(08)01994-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; NEOPLASMS; PLASMIDS; PROSTATE; PUROMYCIN; SCREENING; THERAPY

Citation Formats

Chau, My N., and Banerjee, Partha P. Development of a STAT3 reporter prostate cancer cell line for high throughput screening of STAT3 activators and inhibitors. United States: N. p., 2008. Web. doi:10.1016/j.bbrc.2008.10.025.
Chau, My N., & Banerjee, Partha P. Development of a STAT3 reporter prostate cancer cell line for high throughput screening of STAT3 activators and inhibitors. United States. doi:10.1016/j.bbrc.2008.10.025.
Chau, My N., and Banerjee, Partha P. Fri . "Development of a STAT3 reporter prostate cancer cell line for high throughput screening of STAT3 activators and inhibitors". United States. doi:10.1016/j.bbrc.2008.10.025.
@article{osti_21255787,
title = {Development of a STAT3 reporter prostate cancer cell line for high throughput screening of STAT3 activators and inhibitors},
author = {Chau, My N. and Banerjee, Partha P.},
abstractNote = {STAT3 is constitutively activated in several cancers, including prostate cancer, and is therefore, a potential target for cancer therapy. DU-145 prostate cancer cells were stably co-transfected with STAT3 reporter and puromycin resistant plasmids to create a stable STAT3 reporter cell line that can be used for high throughput screening of STAT3 modulators. The applicability of this cell line was tested with two known activators and inhibitors of STAT3. As expected, EGF and IL-6 increased STAT3 reporter activity and enhanced the nuclear localization of phosphorylated STAT3 (pSTAT3); whereas Cucurbitacin I and AG490 decreased STAT3 reporter activity dose and time-dependently and reduced the localization of pSTAT3 in the nuclei of prostate cancer cells. Given the importance of STAT3 in cancer initiation and progression, the development of a stable STAT3 reporter cell line in prostate cancer cells provides a rapid, sensitive, and cost effective method for the screening of potential STAT3 modulators.},
doi = {10.1016/j.bbrc.2008.10.025},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 377,
place = {United States},
year = {2008},
month = {12}
}