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Title: Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio)

Abstract

Carbon nanotubes (CNTs) are widely explored for biomedical applications, but there is very limited information regarding their in vivo biodistribution and biocompatibility. Here, we report the in vivo biodistribution and long-term effects of functionalized multi-walled carbon nanotubes (MWCNTs) in developing zebrafish. The fluorescent-labeled MWCNTs were introduced into zebrafish embryos at 1-cell stage and at 72 h post fertilization through microinjection. After single injection, both acute and long-term interactions between zebrafish and functionalized MWCNTs were studied. The injected FITC-BSA-MWCNTs (at 1-cell stage) were allocated to all blastoderm cells of the embryos through proliferation, and were distinctively excluded from the yolk cell. When introduced into the circulation system, FITC-BSA-MWCNTs moved easily in the compartments and finally were cleaned out by the body at 96 h after the loading. At early stages, the treated zebrafish embryos generated immune response by accumulating circulating white blood cells at the trunk region. Under transmission electron microscope, many lysosome-like vesicles were observed in the blastoderm cells of the treated embryos. The zebrafish loaded with MWCNTs had normal primordial germ cells at early stage and produced second generation later on. However, the larvae of the second generation had obviously lower survival rates as compared to the untreated groups,more » suggesting a negative effect on the reproduction potential. These results suggest that extensive purification and functionalization processes can help improve the biocompatibility of CNTs. This study also indicates that purified CNTs may have long-term toxicity effects when they were delivered into the body.« less

Authors:
;  [1];  [2];  [3];  [4];  [2]
  1. Department of Biology and Chemistry, City University of Hong Kong (Hong Kong)
  2. Department of Chemistry and Laboratory for Emerging Materials and Technology, Clemson University, Clemson, South Carolina 29634-0973 (United States)
  3. Department of Biochemistry (Science), Chinese University of Hong Kong (Hong Kong)
  4. Department of Knowledge Transfer Office, City University of Hong Kong (Hong Kong)
Publication Date:
OSTI Identifier:
21182749
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 235; Journal Issue: 2; Other Information: DOI: 10.1016/j.taap.2008.12.006; PII: S0041-008X(08)00510-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLOOD CELLS; CARBON; EGGS; EMBRYOS; FERTILIZATION; GERM CELLS; IN VIVO; LARVAE; NANOTUBES; REPRODUCTION; TOXICITY; TRANSMISSION ELECTRON MICROSCOPY

Citation Formats

Jinping, Cheng, Chan, C M, Veca, L Monica, Poon, W L, Chan, P K, Liangwei, Qu, Sun Yaping, and Cheng, S.H. Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio). United States: N. p., 2009. Web. doi:10.1016/j.taap.2008.12.006.
Jinping, Cheng, Chan, C M, Veca, L Monica, Poon, W L, Chan, P K, Liangwei, Qu, Sun Yaping, & Cheng, S.H. Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio). United States. https://doi.org/10.1016/j.taap.2008.12.006
Jinping, Cheng, Chan, C M, Veca, L Monica, Poon, W L, Chan, P K, Liangwei, Qu, Sun Yaping, and Cheng, S.H. 2009. "Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio)". United States. https://doi.org/10.1016/j.taap.2008.12.006.
@article{osti_21182749,
title = {Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio)},
author = {Jinping, Cheng and Chan, C M and Veca, L Monica and Poon, W L and Chan, P K and Liangwei, Qu and Sun Yaping and Cheng, S.H.},
abstractNote = {Carbon nanotubes (CNTs) are widely explored for biomedical applications, but there is very limited information regarding their in vivo biodistribution and biocompatibility. Here, we report the in vivo biodistribution and long-term effects of functionalized multi-walled carbon nanotubes (MWCNTs) in developing zebrafish. The fluorescent-labeled MWCNTs were introduced into zebrafish embryos at 1-cell stage and at 72 h post fertilization through microinjection. After single injection, both acute and long-term interactions between zebrafish and functionalized MWCNTs were studied. The injected FITC-BSA-MWCNTs (at 1-cell stage) were allocated to all blastoderm cells of the embryos through proliferation, and were distinctively excluded from the yolk cell. When introduced into the circulation system, FITC-BSA-MWCNTs moved easily in the compartments and finally were cleaned out by the body at 96 h after the loading. At early stages, the treated zebrafish embryos generated immune response by accumulating circulating white blood cells at the trunk region. Under transmission electron microscope, many lysosome-like vesicles were observed in the blastoderm cells of the treated embryos. The zebrafish loaded with MWCNTs had normal primordial germ cells at early stage and produced second generation later on. However, the larvae of the second generation had obviously lower survival rates as compared to the untreated groups, suggesting a negative effect on the reproduction potential. These results suggest that extensive purification and functionalization processes can help improve the biocompatibility of CNTs. This study also indicates that purified CNTs may have long-term toxicity effects when they were delivered into the body.},
doi = {10.1016/j.taap.2008.12.006},
url = {https://www.osti.gov/biblio/21182749}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 2,
volume = 235,
place = {United States},
year = {Sun Mar 01 00:00:00 EST 2009},
month = {Sun Mar 01 00:00:00 EST 2009}
}