Quantitative Imaging of Single, Unstained Viruses with Coherent X Rays
Journal Article
·
· Physical Review Letters
- Department of Physics and Astronomy, University of California, Los Angeles, California 90095 (United States)
- Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California 90095 (United States)
- Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, Texas 77030 (United States)
- Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095 (United States)
- RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo, Hyogo 679-5148 (Japan)
We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular organelles. Moreover, our experiment is directly transferable to the use of x-ray free electron lasers, and represents an experimental milestone towards the x-ray imaging of large protein complexes.
- OSTI ID:
- 21179805
- Journal Information:
- Physical Review Letters, Vol. 101, Issue 15; Other Information: DOI: 10.1103/PhysRevLett.101.158101; (c) 2008 The American Physical Society; Country of input: International Atomic Energy Agency (IAEA); ISSN 0031-9007
- Country of Publication:
- United States
- Language:
- English
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