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Title: Ultrastructural Study on Ultra-Low Frequency Electromagnetic Fields and Transfer Factor Effects on Skin Ulcers

Abstract

We determined the effect of 120Hz ultra low frequency electromagnetic field (ELF) on the healing process of skin in 20 Wistar rats distributed in four groups in which chronic dermal ulcers had been produced. The first two groups received a dose of the transfer factor and interferon-beta (IFN-{beta}) every 24 h during 12 days. The third group (positive control) received only electromagnetic field (ELF) sessions, and in the fourth group (negative control), no treatment was applied. The electromagnetic field was applied through a Helmholtz coils; 30 Gauss of intensity. Results shown histological changes that improve the healing process in animals subjected to ELF together with the transfer factor.

Authors:
; ;  [1]; ;  [2]; ; ; ; ;  [3]; ;  [4]
  1. Lab. de Bioquimica Muscular, Instituto Nacional de Rehabilitacion, C.P.14389, Mexico, D.F. (Mexico)
  2. Unidad Profesional Interdisciplinaria de Ingenieria y Tecnologias Avanzadas Instituto Politecnico Nacional, C.P. 07340, Mexico, D.F. 07000 (Mexico)
  3. Esc. Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, C.P. 07738, Mexico, D.F. (Mexico)
  4. Depto. de Fisica, CINVESTAV-IPN, Ap. Post. 14-740, Mexico, D.F. 07000 (Mexico)
Publication Date:
OSTI Identifier:
21149269
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1032; Journal Issue: 1; Conference: 10. Mexican symposium on medical physics, Mexico City (Mexico), 17-19 Mar 2008; Other Information: DOI: 10.1063/1.2979267; (c) 2008 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BIOLOGICAL RADIATION EFFECTS; CONTROL; DISEASES; DOSES; ELECTROMAGNETIC FIELDS; HEALING; HZ RANGE; INTERFERON; IRRADIATION; RADIOTHERAPY; RATS; SKIN; ULCERS

Citation Formats

Cadena, M. S. Reyes, Chapul, L. Sanchez, Perez, Javier, Garcia, M. N. Jimenez, Lopez, M. A. Jimenez, Espindola, M. E. Sanchez, Perez, R. Paniagua, Hernandez, N. A., Paniagua, G., Uribe, F., Nava, J. J. Godina, and Segura, M. A. Rodriguez. Ultrastructural Study on Ultra-Low Frequency Electromagnetic Fields and Transfer Factor Effects on Skin Ulcers. United States: N. p., 2008. Web. doi:10.1063/1.2979267.
Cadena, M. S. Reyes, Chapul, L. Sanchez, Perez, Javier, Garcia, M. N. Jimenez, Lopez, M. A. Jimenez, Espindola, M. E. Sanchez, Perez, R. Paniagua, Hernandez, N. A., Paniagua, G., Uribe, F., Nava, J. J. Godina, & Segura, M. A. Rodriguez. Ultrastructural Study on Ultra-Low Frequency Electromagnetic Fields and Transfer Factor Effects on Skin Ulcers. United States. doi:10.1063/1.2979267.
Cadena, M. S. Reyes, Chapul, L. Sanchez, Perez, Javier, Garcia, M. N. Jimenez, Lopez, M. A. Jimenez, Espindola, M. E. Sanchez, Perez, R. Paniagua, Hernandez, N. A., Paniagua, G., Uribe, F., Nava, J. J. Godina, and Segura, M. A. Rodriguez. Mon . "Ultrastructural Study on Ultra-Low Frequency Electromagnetic Fields and Transfer Factor Effects on Skin Ulcers". United States. doi:10.1063/1.2979267.
@article{osti_21149269,
title = {Ultrastructural Study on Ultra-Low Frequency Electromagnetic Fields and Transfer Factor Effects on Skin Ulcers},
author = {Cadena, M. S. Reyes and Chapul, L. Sanchez and Perez, Javier and Garcia, M. N. Jimenez and Lopez, M. A. Jimenez and Espindola, M. E. Sanchez and Perez, R. Paniagua and Hernandez, N. A. and Paniagua, G. and Uribe, F. and Nava, J. J. Godina and Segura, M. A. Rodriguez},
abstractNote = {We determined the effect of 120Hz ultra low frequency electromagnetic field (ELF) on the healing process of skin in 20 Wistar rats distributed in four groups in which chronic dermal ulcers had been produced. The first two groups received a dose of the transfer factor and interferon-beta (IFN-{beta}) every 24 h during 12 days. The third group (positive control) received only electromagnetic field (ELF) sessions, and in the fourth group (negative control), no treatment was applied. The electromagnetic field was applied through a Helmholtz coils; 30 Gauss of intensity. Results shown histological changes that improve the healing process in animals subjected to ELF together with the transfer factor.},
doi = {10.1063/1.2979267},
journal = {AIP Conference Proceedings},
number = 1,
volume = 1032,
place = {United States},
year = {Mon Aug 11 00:00:00 EDT 2008},
month = {Mon Aug 11 00:00:00 EDT 2008}
}
  • The organ cultures of standardized epithelial wound of rabbit cornea, were exposed to electric and magnetic fields components of the extremely low frequency (60 Hz) sinusoidal electromagnetic field, separately or in combination. The electric field was applied as a pure electric current via an agar-salt bridge, and the magnetic field component was applied as a pure polarized magnetic field by means of a pair of energized coils in a Helmholtz configuration. The two field components were at right angles to each other and in phase (phi = 0 degrees) when applied in combination. Their vectors or planes of polarization weremore » parallel to the surface of the culture dish. The three exposure combinations used were: (a) electric current density of 0.19 V/m (30 microA/cm2) and/or magnetic fields strength of 1.0 Gauss. (low intensity) (b) electric current density of 0.31 V/m (50 microA/cm2) and/or magnetic fields strength of 1.5 Gauss. (medium intensity) (c) electric current density of 0.57 V/m (90 microA/cm2) and/or magnetic fields strength of 2.5 Gauss. (high intensity). After 2 days of exposure the incorporation of radiolabelled thymidine into DNA of the regenerating epithelial cells was determined. Results showed thymidine incorporation (adjusted by regression for differences in the levels of DNA in corneal wound) (DNA*) depended upon the electromagnetic field strength. The only significant effect on this outcome, was an interaction effect between the electric (E) and magnetic (M) fields employed. The size and direction of this interaction depended strongly on the maximum field strengths. At the lowest level DNA* was significantly lower when both fields were present than would be expected from the effects of either field alone. At the highest level, DNA* was significantly higher. There were no significant effects for medium field strengths.« less
  • Prolonged exposure of the myxomycete Physarum polycephalum to either continuous wave (75 Hz) or frequency modulated wave (76 Hz) electromagnetic fields (EMF) (0.1 to 2.0 G and 0.035 to 0.7 V/m) lengthens the mitotic cycle and depresses the respiration rate. Once induced, these effects persist indefinately in the presence of EMF without increasing or decreasing in magnitude beyond that due to normal variability of the organism. Similar effects are observed when either individual electric fields (0.7 V/m) or magnetic fields (2.0 G) are applied; however, the magnitude of the response is less than that observed with simultaneous fields. The individualmore » field effects appear to be additive for respiration but not for nuclear division rate. For fields applied simultaneously at levels below 0.14 V/m and 0.4 G the response was independent of field intensity. No threshold was observed for simultaneously applied electric and magnetic fields; however, indirect evidence is presented that suggests either the electric or magnetic field is below threshold at levels of 0.14 V/m and 0.4 G, respectively. Frequency modulation of the fields seems to have no major effect o the response induced in P. polycephalum.« less
  • We conducted this study to determine if electromagnetic (EM) fields produced by an extremely low frequency (ELF) antenna system affected either the abundance or richness of breeding and migrating bird species. We counted birds on 80-500 m transects, 40 in reference areas and 40 in treatment areas adjacent to an ELF antenna system in northern Wisconsin. Counts were done three times during the breeding season and twice during autumn migration from 1986 to 1989. We used repeated measures ANOVA (multivariate test) to determine if species abundance and numbers of species varied annually (univariate test), no treatment effects were detected formore » bird community parameters. Fifteen of 75 breeding bird species tested indicated significant differences between reference and treatment study areas; 10 were more abundant in treatment areas. Six of 10 species more abundant in treatment areas were species that occur along edges. Eight of 51 species showed significant treatment effects in the migration season; six were more abundant in reference study areas. Two species, the ruffed grouse (Bonasa umbellus) and white-breasted nuthatch (Sitta carolinensis) were more abundant in reference study areas in both the breeding and migration seasons. Overall, most differences detected between treatment and reference study areas could be attributed to differences in habitat (or presence of edge). It is unlikely that differences could be attributed to ELF EM fields. 49 refs., 4 figs., 1 tab.« less
  • Absolute reaction rate theory is applied to explain the increased time between successive synchronous mitotic nuclear divisions in the slime mold Physarum polycephalum when exposed to extremely low-frequency electromagnetic fields. By comparing the normal (control) mitotic cycle to the lengthened mitotic cycle of exposed P. polycephalum, the change in the activation energy is established. This activation energy is in the range of enzyme-catalyzed reactions. The influence of changing frequency on the transition period from normal to lengthened mitotic cycle is considered to complete the model. Suggestions are made for further experimentation.