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Title: Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein

Abstract

Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells.

Authors:
 [1];  [2]; ;  [2];  [3];  [3];  [1]
  1. Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120 (Thailand)
  2. Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building (12th Floor), Mahidol University, Bangkok 10700 (Thailand)
  3. Institute of Biological Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan (China)
Publication Date:
OSTI Identifier:
21143767
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 372; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2008.04.165; PII: S0006-291X(08)00858-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DISEASES; ELECTROPHORESIS; GELS; GLYCOPROTEINS; KIDNEYS; MASS SPECTROSCOPY; MONOCLONAL ANTIBODIES; PATHOGENESIS; QUADRUPOLES; VIRUSES

Citation Formats

Noisakran, Sansanee, Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building, Sengsai, Suchada, Thongboonkerd, Visith, Kanlaya, Rattiyaporn, Medical Proteomics Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Sinchaikul, Supachok, Chen, Shui-Tein, Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan, Puttikhunt, Chunya, and Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building. Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein. United States: N. p., 2008. Web. doi:10.1016/j.bbrc.2008.04.165.
Noisakran, Sansanee, Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building, Sengsai, Suchada, Thongboonkerd, Visith, Kanlaya, Rattiyaporn, Medical Proteomics Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Sinchaikul, Supachok, Chen, Shui-Tein, Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan, Puttikhunt, Chunya, & Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building. Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein. United States. doi:10.1016/j.bbrc.2008.04.165.
Noisakran, Sansanee, Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building, Sengsai, Suchada, Thongboonkerd, Visith, Kanlaya, Rattiyaporn, Medical Proteomics Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Sinchaikul, Supachok, Chen, Shui-Tein, Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan, Puttikhunt, Chunya, and Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building. Fri . "Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein". United States. doi:10.1016/j.bbrc.2008.04.165.
@article{osti_21143767,
title = {Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein},
author = {Noisakran, Sansanee and Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building and Sengsai, Suchada and Thongboonkerd, Visith and Kanlaya, Rattiyaporn and Medical Proteomics Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 and Sinchaikul, Supachok and Chen, Shui-Tein and Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan and Puttikhunt, Chunya and Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building},
abstractNote = {Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells.},
doi = {10.1016/j.bbrc.2008.04.165},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 372,
place = {United States},
year = {2008},
month = {7}
}