A proton-activated, outwardly rectifying chloride channel in human umbilical vein endothelial cells
- Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology QiLu Hospital Shandong University, West Wenhua Road 107, Jinan 250012 (China)
Extracellular acidic pH-activated chloride channel I{sub Cl,acid}, has been characterized in HEK 293 cells and mammalian cardiac myocytes. This study was designed to characterize I{sub Cl,acid} in human umbilical vein endothelial cells(HUVECs). The activation and deactivation of the current rapidly and repeatedly follows the change of the extracellular solution at pH 4.3, with the threshold pH 5.3. In addition, at very positive potentials, the current displays a time-dependent facilitation. pH-response relationship for I{sub Cl,acid} revealed that EC{sub 50} is pH 4.764 with a threshold pH value of pH 5.3 and nH of 14.545. The current can be blocked by the Cl{sup -} channel inhibitor DIDS (100 {mu}M). In summary, for the first time we report the presence of proton-activated, outwardly rectifying chloride channel in HUVECs. Because an acidic environment can develop in local myocardium under pathological conditions such as myocardial ischemia, I{sub Cl,acid} would play a role in regulation of EC function under these pathological conditions.
- OSTI ID:
- 21143740
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 371, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2008.04.090; PII: S0006-291X(08)00774-2; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Binding of tissue plasminogen activator to human umbilical vein endothelial cells
Exosomes derived from human umbilical vein endothelial cells promote neural stem cell expansion while maintain their stemness in culture