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Title: HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells

Abstract

Prolactinoma is one of the most common types of pituitary adenoma. It has been reported that a variety of growth factors and cytokines regulating cell growth and angiogenesis play an important role in the growth of prolactinoma. HoxD10 has been shown to impair endothelial cell migration, block angiogenesis, and maintain a differentiated phenotype of cells. We investigated whether HoxD10 gene delivery could inhibit the growth of prolactinoma. Rat GH4 lactotrope tumor cells were infected with adenovirus/adeno-associated virus (Ad/AAV) hybrid vectors carrying the mouse HoxD10 gene (Hyb-HoxD10) or the {beta}-galactosidase gene (Hyb-Gal). Hyb-HoxD10 expression inhibited GH4 cell proliferation in vitro. The expression of FGF-2 and cyclin D2 was inhibited in GH4 cells infected with Hyb-HoxD10. GH4 cells transduced with Hyb-HoxD10 did not form tumors in nude mice. These results indicate that the delivery of HoxD10 could potentially inhibit the growth of PRL-secreting tumors. This approach may be a useful tool for targeted therapy of prolactinoma and other neoplasms.

Authors:
 [1];  [2]; ;  [1];  [2];  [1];  [2]
  1. Division of Endocrinology, Pituitary Tumor Clinic, Research Institute of Endocrinology, Yonsei University College of Medicine, Seoul (Korea, Republic of)
  2. Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States)
Publication Date:
OSTI Identifier:
21143737
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 371; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2008.04.085; PII: S0006-291X(08)00731-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADENOMAS; ADENOVIRUS; CELL PROLIFERATION; FIBROBLASTS; GALACTOSIDASE; GENES; IN VITRO; LYMPHOKINES; MICE; RATS; TUMOR CELLS

Citation Formats

Cho, Mi Ae, Endocrinology, Dong Rae Bong Seng Hospital, Busan, Yashar, Parham, Department of Neurosurgery, University of Southern California - Keck School of Medicine, Los Angeles, CA, Kim, Suk Kyoung, Noh, Taewoong, Gillam, Mary P, Lee, Eun Jig, Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, and Jameson, J Larry. HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells. United States: N. p., 2008. Web. doi:10.1016/j.bbrc.2008.04.085.
Cho, Mi Ae, Endocrinology, Dong Rae Bong Seng Hospital, Busan, Yashar, Parham, Department of Neurosurgery, University of Southern California - Keck School of Medicine, Los Angeles, CA, Kim, Suk Kyoung, Noh, Taewoong, Gillam, Mary P, Lee, Eun Jig, Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, & Jameson, J Larry. HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells. United States. https://doi.org/10.1016/j.bbrc.2008.04.085
Cho, Mi Ae, Endocrinology, Dong Rae Bong Seng Hospital, Busan, Yashar, Parham, Department of Neurosurgery, University of Southern California - Keck School of Medicine, Los Angeles, CA, Kim, Suk Kyoung, Noh, Taewoong, Gillam, Mary P, Lee, Eun Jig, Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, and Jameson, J Larry. 2008. "HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells". United States. https://doi.org/10.1016/j.bbrc.2008.04.085.
@article{osti_21143737,
title = {HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells},
author = {Cho, Mi Ae and Endocrinology, Dong Rae Bong Seng Hospital, Busan and Yashar, Parham and Department of Neurosurgery, University of Southern California - Keck School of Medicine, Los Angeles, CA and Kim, Suk Kyoung and Noh, Taewoong and Gillam, Mary P and Lee, Eun Jig and Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 and Jameson, J Larry},
abstractNote = {Prolactinoma is one of the most common types of pituitary adenoma. It has been reported that a variety of growth factors and cytokines regulating cell growth and angiogenesis play an important role in the growth of prolactinoma. HoxD10 has been shown to impair endothelial cell migration, block angiogenesis, and maintain a differentiated phenotype of cells. We investigated whether HoxD10 gene delivery could inhibit the growth of prolactinoma. Rat GH4 lactotrope tumor cells were infected with adenovirus/adeno-associated virus (Ad/AAV) hybrid vectors carrying the mouse HoxD10 gene (Hyb-HoxD10) or the {beta}-galactosidase gene (Hyb-Gal). Hyb-HoxD10 expression inhibited GH4 cell proliferation in vitro. The expression of FGF-2 and cyclin D2 was inhibited in GH4 cells infected with Hyb-HoxD10. GH4 cells transduced with Hyb-HoxD10 did not form tumors in nude mice. These results indicate that the delivery of HoxD10 could potentially inhibit the growth of PRL-secreting tumors. This approach may be a useful tool for targeted therapy of prolactinoma and other neoplasms.},
doi = {10.1016/j.bbrc.2008.04.085},
url = {https://www.osti.gov/biblio/21143737}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 371,
place = {United States},
year = {Fri Jul 04 00:00:00 EDT 2008},
month = {Fri Jul 04 00:00:00 EDT 2008}
}