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Title: Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain

Abstract

Human mesenchymal stem cell-adhesive peptides were screened based on the amino acid sequence of fibronectin type III domain 8-11 (FN-III{sub 8-11}) using a peptide array synthesized by the Fmoc-chemistry. Using hexameric peptide library of FN-III{sub 8-11} scan, we identified the ALNGR (Ala-Leu-Asn-Gly-Arg) peptide that induced cell adhesion as well as RGDS (Arg-Gly-Asp-Ser) peptide. After incubation for 2 h, approximately 68% of inoculated cells adhere to the ALNGR peptide disk. Adhesion inhibition assay with integrin antibodies showed that the ALNGR peptide interacts with integrin {beta}1 but not with {alpha}v{beta}3, indicating that the receptors for ALNGR are different from RGDS. Additionally, the ALNGR peptide expressed cell specificities for adhesion: cell adhesion was promoted for fibroblasts but not for keratinocytes or endotherial cells. The ALNGR peptide induced cell adhesion and promoted cell proliferation without changing its property. It is therefore useful for the construction of functional biomaterials.

Authors:
; ;  [1];  [1]
  1. Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan)
Publication Date:
OSTI Identifier:
21143723
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 371; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2008.04.019; PII: S0006-291X(08)00652-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADHESION; AMINO ACID SEQUENCE; ANTIBODIES; CELL PROLIFERATION; FIBROBLASTS; PEPTIDES; RECEPTORS; SPECIFICITY; STEM CELLS

Citation Formats

Okochi, Mina, Nomura, Shigeyuki, Kaga, Chiaki, Honda, Hiroyuki, and MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603. Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain. United States: N. p., 2008. Web. doi:10.1016/j.bbrc.2008.04.019.
Okochi, Mina, Nomura, Shigeyuki, Kaga, Chiaki, Honda, Hiroyuki, & MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603. Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain. United States. https://doi.org/10.1016/j.bbrc.2008.04.019
Okochi, Mina, Nomura, Shigeyuki, Kaga, Chiaki, Honda, Hiroyuki, and MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603. 2008. "Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain". United States. https://doi.org/10.1016/j.bbrc.2008.04.019.
@article{osti_21143723,
title = {Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain},
author = {Okochi, Mina and Nomura, Shigeyuki and Kaga, Chiaki and Honda, Hiroyuki and MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603},
abstractNote = {Human mesenchymal stem cell-adhesive peptides were screened based on the amino acid sequence of fibronectin type III domain 8-11 (FN-III{sub 8-11}) using a peptide array synthesized by the Fmoc-chemistry. Using hexameric peptide library of FN-III{sub 8-11} scan, we identified the ALNGR (Ala-Leu-Asn-Gly-Arg) peptide that induced cell adhesion as well as RGDS (Arg-Gly-Asp-Ser) peptide. After incubation for 2 h, approximately 68% of inoculated cells adhere to the ALNGR peptide disk. Adhesion inhibition assay with integrin antibodies showed that the ALNGR peptide interacts with integrin {beta}1 but not with {alpha}v{beta}3, indicating that the receptors for ALNGR are different from RGDS. Additionally, the ALNGR peptide expressed cell specificities for adhesion: cell adhesion was promoted for fibroblasts but not for keratinocytes or endotherial cells. The ALNGR peptide induced cell adhesion and promoted cell proliferation without changing its property. It is therefore useful for the construction of functional biomaterials.},
doi = {10.1016/j.bbrc.2008.04.019},
url = {https://www.osti.gov/biblio/21143723}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 371,
place = {United States},
year = {Fri Jun 20 00:00:00 EDT 2008},
month = {Fri Jun 20 00:00:00 EDT 2008}
}