Selective structural change of bulged-out region of double-stranded RNA containing bulged nucleotides by spermidine
- Department of Clinical Biochemistry, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675 (Japan)
- Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi-shi, Chiba 288-0025 (Japan)
- Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-8588 (Japan)
Polyamines are essential for cell growth due to effects mainly at the level of translation. These effects likely involve a structural change, induced by polyamines, of the bulged-out region of double-stranded RNA that is different from changes induced by Mg{sup 2+}. Structural changes were studied using U6-34, a model RNA of U6 small nuclear RNA containing bulged nucleotides. Binding of NS1-2 peptide derived from the RNA binding site of NS1 protein, to U6-34 was inhibited by spermidine but not by Mg{sup 2+}. A selective conformational change of the bases in the bulged-out region of U6-34 induced by spermidine was observed by NMR. The selective effect of spermidine was lost when the bulged-out region of U6-34 was removed in U6-34({delta}5). The binding of NS1-2 peptide to U6-34({delta}5) was inhibited both by spermidine and Mg{sup 2+}. The selective structural change of U6-34 by spermidine was confirmed by circular dichroism.
- OSTI ID:
- 21143717
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 370, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.03.137; PII: S0006-291X(08)00609-8; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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