NHE10, a novel osteoclast-specific member of the Na{sup +}/H{sup +} exchanger family, regulates osteoclast differentiation and survival

Journal Article · Fri May 02 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.01.168· OSTI ID:21143641

Lee, Seoung Hoon; Kim, Taesoo ^[1]; Park, Eui-Soon; Yang, Sujeong ^[2]; Jeong, Daewon ^[3]; Choi, Yongwon ^[1]; Rho, Jaerang ^[2]

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1. Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 (United States)
2. Department of Microbiology, RCTCP, and BK21 BioBC, Chungnam National University, 220 Gungdong, Yuseonggu, Daejon 305-764 (Korea, Republic of)
3. Department of Microbiology, Yeungnam University College of Medicine, 317-1 Daemyungdong, Namgu, Daegu 705-717 (Korea, Republic of)

Bone homeostasis is tightly regulated by the balanced actions of osteoblasts (OBs) and osteoclasts (OCs). We previously analyzed the gene expression profile of OC differentiation using a cDNA microarray, and identified a novel osteoclastogenic gene candidate, clone OCL-1-E7 [J. Rho, C.R. Altmann, N.D. Socci, L. Merkov, N. Kim, H. So, O. Lee, M. Takami, A.H. Brivanlou, Y. Choi, Gene expression profiling of osteoclast differentiation by combined suppression subtractive hybridization (SSH) and cDNA microarray analysis, DNA Cell Biol. 21 (2002) 541-549]. In this study, we have isolated full-length cDNAs corresponding to this clone from mice and humans to determine the functional roles of this gene in osteoclastogenesis. The full-length cDNA of OCL-1-E7 encodes 12 membrane-spanning domains that are typical of isoforms of the Na{sup +}/H{sup +} exchangers (NHEs), indicating that this clone is a novel member of the NHE family (hereafter referred to as NHE10). Here, we show that NHE10 is highly expressed in OCs in response to receptor activator of nuclear factor-{kappa}B ligand signaling and is required for OC differentiation and survival.

• OSTI ID: 21143641
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 369, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2008.01.168; PII: S0006-291X(08)00237-4; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

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60 APPLIED LIFE SCIENCES
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GENES
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HYBRIDIZATION
INHIBITION
LIGANDS
MACROPHAGES
MICE
RECEPTORS
SKELETON
SODIUM IONS