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Title: Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-{kappa}B activation

Abstract

Styrene is a volatile organic compound (VOC) that is widely used as a solvent in many industrial settings. Chronic exposure to styrene can result in irritation of the mucosa of the upper respiratory tract. Contact of styrene with epithelial cells stimulates the expression of a variety of inflammatory mediators, including the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1). To characterise the underlying mechanisms of the induction of inflammatory signals by styrene, we investigated the influence of this compound on the induction of oxidative stress and the activation of the nuclear factor-kappa B (NF-{kappa}B) signalling pathway in human lung epithelial cells (A549). The results demonstrate that styrene-induced MCP-1 expression, as well as the expression of the oxidative stress marker glutathione S-transferase (GST), is associated with a concentration dependent pattern of NF-{kappa}B activity. An inhibitor of NF-{kappa}B, IKK-NBD, and the anti-inflammatory antioxidant N-acetylcysteine (NAC) were both effective in suppressing styrene-induced MCP-1 secretion. In addition, NAC was capable of inhibiting the upregulation of GST expression. Our findings suggest that the activation of the NF-{kappa}B signalling pathway by styrene is mediated via a redox-sensitive mechanism.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [8];  [9];  [10]
  1. UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany), E-mail: carmen.roeder-stolinski@ufz.de
  2. UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany), E-mail: gundula.fischaeder@ufz.de
  3. University of Salzburg, Department of Molecular Biology, A-5020 Salzburg (Austria), E-mail: geja.oostingh@sbg.ac.at
  4. UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany), E-mail: ralph.feltens@ufz.de
  5. UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany)
  6. UFZ - Helmholtz Centre for Environmental Research, Department of Proteomics, Permoserstrasse 15, D-04318 Leipzig (Germany), E-mail: martin.vonbergen@ufz.de
  7. UFZ - Helmholtz Centre for Environmental Research, Department of Proteomics, Permoserstrasse 15, D-04318 Leipzig (Germany), E-mail: nora.moerbt@ufz.de
  8. Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, D-06108 Halle (Saale) (Germany), E-mail: klaus.eder@landw.uni-halle.de
  9. University of Salzburg, Department of Molecular Biology, A-5020 Salzburg (Austria), E-mail: albert.duschl@sbg.ac.at
  10. UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany), E-mail: irina.lehmann@ufz.de
Publication Date:
OSTI Identifier:
21140947
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 231; Journal Issue: 2; Other Information: DOI: 10.1016/j.taap.2008.04.010; PII: S0041-008X(08)00181-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIOXIDANTS; CHRONIC EXPOSURE; GLUTATHIONE; INFLAMMATION; LUNGS; MONOCYTES; MUCOUS MEMBRANES; OXIDATION; PHOSPHATES; SECRETION; STYRENE; VOLATILE MATTER; VOLATILITY

Citation Formats

Roeder-Stolinski, Carmen, Fischaeder, Gundula, Oostingh, Gertie Janneke, Feltens, Ralph, Kohse, Franziska, Bergen, Martin von, Moerbt, Nora, Eder, Klaus, Duschl, Albert, and Lehmann, Irina. Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-{kappa}B activation. United States: N. p., 2008. Web. doi:10.1016/j.taap.2008.04.010.
Roeder-Stolinski, Carmen, Fischaeder, Gundula, Oostingh, Gertie Janneke, Feltens, Ralph, Kohse, Franziska, Bergen, Martin von, Moerbt, Nora, Eder, Klaus, Duschl, Albert, & Lehmann, Irina. Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-{kappa}B activation. United States. doi:10.1016/j.taap.2008.04.010.
Roeder-Stolinski, Carmen, Fischaeder, Gundula, Oostingh, Gertie Janneke, Feltens, Ralph, Kohse, Franziska, Bergen, Martin von, Moerbt, Nora, Eder, Klaus, Duschl, Albert, and Lehmann, Irina. Mon . "Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-{kappa}B activation". United States. doi:10.1016/j.taap.2008.04.010.
@article{osti_21140947,
title = {Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-{kappa}B activation},
author = {Roeder-Stolinski, Carmen and Fischaeder, Gundula and Oostingh, Gertie Janneke and Feltens, Ralph and Kohse, Franziska and Bergen, Martin von and Moerbt, Nora and Eder, Klaus and Duschl, Albert and Lehmann, Irina},
abstractNote = {Styrene is a volatile organic compound (VOC) that is widely used as a solvent in many industrial settings. Chronic exposure to styrene can result in irritation of the mucosa of the upper respiratory tract. Contact of styrene with epithelial cells stimulates the expression of a variety of inflammatory mediators, including the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1). To characterise the underlying mechanisms of the induction of inflammatory signals by styrene, we investigated the influence of this compound on the induction of oxidative stress and the activation of the nuclear factor-kappa B (NF-{kappa}B) signalling pathway in human lung epithelial cells (A549). The results demonstrate that styrene-induced MCP-1 expression, as well as the expression of the oxidative stress marker glutathione S-transferase (GST), is associated with a concentration dependent pattern of NF-{kappa}B activity. An inhibitor of NF-{kappa}B, IKK-NBD, and the anti-inflammatory antioxidant N-acetylcysteine (NAC) were both effective in suppressing styrene-induced MCP-1 secretion. In addition, NAC was capable of inhibiting the upregulation of GST expression. Our findings suggest that the activation of the NF-{kappa}B signalling pathway by styrene is mediated via a redox-sensitive mechanism.},
doi = {10.1016/j.taap.2008.04.010},
journal = {Toxicology and Applied Pharmacology},
number = 2,
volume = 231,
place = {United States},
year = {Mon Sep 01 00:00:00 EDT 2008},
month = {Mon Sep 01 00:00:00 EDT 2008}
}