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Title: Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS

Abstract

Perfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5 mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5 mg/L PFOS until 132 hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1 mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5 mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex andmore » pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1 mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity.« less

Authors:
 [1];  [2];  [1]; ;  [3];  [4]
  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072 (China)
  2. (China)
  3. Department of Biology and Chemistry, City University of Hong Kong (China)
  4. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072 (China), E-mail: bszhou@ihb.ac.cn
Publication Date:
OSTI Identifier:
21140876
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 230; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2008.01.043; PII: S0041-008X(08)00061-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACRIDINE ORANGE; ANDROGENS; APOPTOSIS; DNA; EDEMA; EGGS; EMBRYOS; ESTROGENS; FERTILIZATION; GENES; HEART; MALFORMATIONS; POLLUTANTS; THYROID; TOXICITY

Citation Formats

Shi Xiongjie, Graduate School of the Chinese Academy of Sciences, Beijing 100039, Du Yongbing, Lam, Paul K.S., Wu, Rudolf S.S., and Zhou Bingsheng. Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS. United States: N. p., 2008. Web.
Shi Xiongjie, Graduate School of the Chinese Academy of Sciences, Beijing 100039, Du Yongbing, Lam, Paul K.S., Wu, Rudolf S.S., & Zhou Bingsheng. Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS. United States.
Shi Xiongjie, Graduate School of the Chinese Academy of Sciences, Beijing 100039, Du Yongbing, Lam, Paul K.S., Wu, Rudolf S.S., and Zhou Bingsheng. Tue . "Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS". United States.
@article{osti_21140876,
title = {Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS},
author = {Shi Xiongjie and Graduate School of the Chinese Academy of Sciences, Beijing 100039 and Du Yongbing and Lam, Paul K.S. and Wu, Rudolf S.S. and Zhou Bingsheng},
abstractNote = {Perfluorooctanesulfonate (PFOS) is a persistent organic pollutant, the potential toxicity of which is causing great concern. In the present study, we employed zebrafish embryos to investigate the developmental toxicity of this compound. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0.1, 0.5, 1, 3 and 5 mg/L PFOS. Hatching was delayed and hatching rates as well as larval survivorship were significantly reduced after the embryos were exposed to 1, 3 and 5 mg/L PFOS until 132 hpf. The fry displayed gross developmental malformations, including epiboly deformities, hypopigmentation, yolk sac edema, tail and heart malformations and spinal curvature upon exposure to PFOS concentrations of 1 mg/L or greater. Growth (body length) was significantly reduced in the 3 and 5 mg/L PFOS-treated groups. To test whether developmental malformation was mediated via apoptosis, flow cytometry analysis of DNA content, acridine orange staining and TUNEL assay was used. These techniques indicated that more apoptotic cells were present in the PFOS-treated embryos than in the control embryos. Certain genes related to cell apoptosis, p53 and Bax, were both significantly up-regulated upon exposure to all the concentrations tested. In addition, we investigated the effects of PFOS on marker genes related to early thyroid development (hhex and pax8) and genes regulating the balance of androgens and estrogens (cyp19a and cyp19b). For thyroid development, the expression of hhex was significantly up-regulated at all concentrations tested, whereas pax8 expression was significantly up-regulated only upon exposure to lower concentrations of PFOS (0.1, 0.5, 1 mg/L). The expression of cyp19a and of cyp19b was significantly down-regulated at all exposure concentrations. The overall results indicated that zebrafish embryos constitute a reliable model for testing the developmental toxicity of PFOS, and the gene expression patterns in the embryos were able to reveal some potential mechanisms of developmental toxicity.},
doi = {},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 230,
place = {United States},
year = {Tue Jul 01 00:00:00 EDT 2008},
month = {Tue Jul 01 00:00:00 EDT 2008}
}