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Title: DNA repair in murine embryonic stem cells and differentiated cells

Abstract

Embryonic stem (ES) cells are rapidly proliferating, self-renewing cells that have the capacity to differentiate into all three germ layers to form the embryo proper. Since these cells are critical for embryo formation, they must have robust prophylactic mechanisms to ensure that their genomic integrity is preserved. Indeed, several studies have suggested that ES cells are hypersensitive to DNA damaging agents and readily undergo apoptosis to eliminate damaged cells from the population. Other evidence suggests that DNA damage can cause premature differentiation in these cells. Several laboratories have also begun to investigate the role of DNA repair in the maintenance of ES cell genomic integrity. It does appear that ES cells differ in their capacity to repair damaged DNA compared to differentiated cells. This minireview focuses on repair mechanisms ES cells may use to help preserve genomic integrity and compares available data regarding these mechanisms with those utilized by differentiated cells.

Authors:
 [1];  [2]
  1. Department of Cell and Cancer Biology, University of Cincinnati, Cincinnati, OH 45267 (United States), E-mail: tichyed@email.uc.edu
  2. Department of Cell and Cancer Biology, University of Cincinnati, Cincinnati, OH 45267 (United States)
Publication Date:
OSTI Identifier:
21128186
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 314; Journal Issue: 9; Other Information: DOI: 10.1016/j.yexcr.2008.02.007; PII: S0014-4827(08)00083-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; DNA; DNA DAMAGES; EMBRYOS; EXCISION REPAIR; NUCLEOTIDES; STEM CELLS

Citation Formats

Tichy, Elisia D., and Stambrook, Peter J. DNA repair in murine embryonic stem cells and differentiated cells. United States: N. p., 2008. Web. doi:10.1016/j.yexcr.2008.02.007.
Tichy, Elisia D., & Stambrook, Peter J. DNA repair in murine embryonic stem cells and differentiated cells. United States. doi:10.1016/j.yexcr.2008.02.007.
Tichy, Elisia D., and Stambrook, Peter J. Tue . "DNA repair in murine embryonic stem cells and differentiated cells". United States. doi:10.1016/j.yexcr.2008.02.007.
@article{osti_21128186,
title = {DNA repair in murine embryonic stem cells and differentiated cells},
author = {Tichy, Elisia D. and Stambrook, Peter J.},
abstractNote = {Embryonic stem (ES) cells are rapidly proliferating, self-renewing cells that have the capacity to differentiate into all three germ layers to form the embryo proper. Since these cells are critical for embryo formation, they must have robust prophylactic mechanisms to ensure that their genomic integrity is preserved. Indeed, several studies have suggested that ES cells are hypersensitive to DNA damaging agents and readily undergo apoptosis to eliminate damaged cells from the population. Other evidence suggests that DNA damage can cause premature differentiation in these cells. Several laboratories have also begun to investigate the role of DNA repair in the maintenance of ES cell genomic integrity. It does appear that ES cells differ in their capacity to repair damaged DNA compared to differentiated cells. This minireview focuses on repair mechanisms ES cells may use to help preserve genomic integrity and compares available data regarding these mechanisms with those utilized by differentiated cells.},
doi = {10.1016/j.yexcr.2008.02.007},
journal = {Experimental Cell Research},
number = 9,
volume = 314,
place = {United States},
year = {Tue Jun 10 00:00:00 EDT 2008},
month = {Tue Jun 10 00:00:00 EDT 2008}
}