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Title: Combined application of neutron and synchrotron radiation for investigation of the influence of dimethyl sulfoxide on the structure and properties of the dipalmitoylphosphatidylcholine membrane

Abstract

The influence of dimethyl sulfoxide (DMSO) on the structure and properties of the dipalmitoylphosphatidylcholine membrane was studied at positive temperatures by a combination of X-ray diffraction and small-angle neutron scattering. Penetration of DMSO molecules into the lipid membrane was found to depend on the mole fraction of DMSO in an aqueous solution, X{sub DMSO}. At X{sub DMSO} > 0.08 the SO group penetrates into the bilayer polar region, thus resulting in structural alterations. At X{sub DMSO} > 0.2 defects in the membrane surface are developed.

Authors:
 [1]
  1. Joint Institute for Nuclear Research (Russian Federation), E-mail: kiselev@jinr.ru
Publication Date:
OSTI Identifier:
21090901
Resource Type:
Journal Article
Resource Relation:
Journal Name: Crystallography Reports; Journal Volume: 52; Journal Issue: 3; Other Information: DOI: 10.1134/S1063774507030352; Copyright (c) 2007 Nauka/Interperiodica; Article Copyright (c) 2007 Pleiades Publishing, Inc; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; AQUEOUS SOLUTIONS; DMSO; MEMBRANES; NEUTRON DIFFRACTION; PHOSPHOLIPIDS; SMALL ANGLE SCATTERING; SYNCHROTRON RADIATION; X-RAY DIFFRACTION

Citation Formats

Kiselev, M. A.. Combined application of neutron and synchrotron radiation for investigation of the influence of dimethyl sulfoxide on the structure and properties of the dipalmitoylphosphatidylcholine membrane. United States: N. p., 2007. Web. doi:10.1134/S1063774507030352.
Kiselev, M. A.. Combined application of neutron and synchrotron radiation for investigation of the influence of dimethyl sulfoxide on the structure and properties of the dipalmitoylphosphatidylcholine membrane. United States. doi:10.1134/S1063774507030352.
Kiselev, M. A.. Tue . "Combined application of neutron and synchrotron radiation for investigation of the influence of dimethyl sulfoxide on the structure and properties of the dipalmitoylphosphatidylcholine membrane". United States. doi:10.1134/S1063774507030352.
@article{osti_21090901,
title = {Combined application of neutron and synchrotron radiation for investigation of the influence of dimethyl sulfoxide on the structure and properties of the dipalmitoylphosphatidylcholine membrane},
author = {Kiselev, M. A.},
abstractNote = {The influence of dimethyl sulfoxide (DMSO) on the structure and properties of the dipalmitoylphosphatidylcholine membrane was studied at positive temperatures by a combination of X-ray diffraction and small-angle neutron scattering. Penetration of DMSO molecules into the lipid membrane was found to depend on the mole fraction of DMSO in an aqueous solution, X{sub DMSO}. At X{sub DMSO} > 0.08 the SO group penetrates into the bilayer polar region, thus resulting in structural alterations. At X{sub DMSO} > 0.2 defects in the membrane surface are developed.},
doi = {10.1134/S1063774507030352},
journal = {Crystallography Reports},
number = 3,
volume = 52,
place = {United States},
year = {Tue May 15 00:00:00 EDT 2007},
month = {Tue May 15 00:00:00 EDT 2007}
}
  • No abstract prepared.
  • The influence of dimethyl sulfoxide (CH{sub 3}){sub 2}SO (DMSO) on the structure of membranes of 1,2-dimiristoyl-sn-glycero-3-phosphatidylcholine (DMPC) in an excess of a water-DMSO solvent is investigated over a wide range of DMSO molar concentrations 0.0 {<=} X{sub DMSO} {<=} 1.0 at temperatures T = 12.5 and 55 deg. C. The dependences of the repeat distance d of multilamellar membranes and the thickness d{sub b} of single vesicles on the molar concentration X{sub DMSO} in the L{sub {beta}}{sub '} gel and L{sub {alpha}} liquid-crystalline phases are determined by small-angle neutron scattering. The intermembrane distance d{sub s} is determined from the repeatmore » distance d and the membrane thickness d{sub b}. It is shown that an increase in the molar concentration X{sub DMSO} leads to a considerable decrease in the intermembrane distance and that, at X{sub DMSO} = 0.4, the neighboring membranes are virtually in steric contact with each other. The use of the deuterated phospholipid (DMSO-D6) and the contrast variation method makes it possible, for the first time, to determine the number of DMSO molecules strongly bound to the membrane.« less
  • A combination of molecular dynamics simulations and pulsed field gradient nuclear magnetic resonance spectroscopy is used to investigate the role of exogenous electric fields on the solvation structure and dynamics of alkali ions in dimethyl sulfoxide (DMSO) and as a function of temperature. Good agreement was obtained, for select alkali ions in the absence of an electric field, between calculated and experimentally determined diffusion coefficients normalized to that of pure DMSO. Our results indicate that temperatures of up to 400 K and external electric fields of up to 1 V nm{sup −1} have minimal effects on the solvation structure ofmore » the smaller alkali cations (Li{sup +} and Na{sup +}) due to their relatively strong ion-solvent interactions, whereas the solvation structures of the larger alkali cations (K{sup +}, Rb{sup +}, and Cs{sup +}) are significantly affected. In addition, although the DMSO exchange dynamics in the first solvation shell differ markedly for the two groups, the drift velocities and mobilities are not significantly affected by the nature of the alkali ion. Overall, although exogenous electric fields induce a drift displacement, their presence does not significantly affect the random diffusive displacement of the alkali ions in DMSO. System temperature is found to have generally a stronger influence on dynamical properties, such as the DMSO exchange dynamics and the ion mobilities, than the presence of electric fields.« less