skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5

Abstract

Paramyxoviruses have been shown to produce proteins that inhibit interferon production and signaling. For human respiratory syncytial virus (RSV), the nonstructural NS1 and NS2 proteins have been shown to have interferon antagonist activity through an unknown mechanism. To understand further the functions of NS1 and NS2, we generated recombinant RSV in which both NS1 and NS2 were replaced by the PIV5 V protein, which has well-characterized IFN antagonist activities ({delta}NS1/2-V). Expression of V was able to partially inhibit IFN responses in {delta}NS1/2-V-infected cells. In addition, the replication kinetics of {delta}NS1/2-V were intermediate between {delta}NS1/2 and wild-type (rA2) in A549 cells. However, expression of V did not affect the ability of {delta}NS1/2-V to activate IRF3 nuclear translocation and IFN{beta} transcription. These data indicate that V was able to replace some of the IFN inhibitory functions of the RSV NS1 and NS2 proteins, but also that NS1 and NS2 have functions in viral replication beyond IFN antagonism.

Authors:
 [1];  [2];  [3]
  1. Department of Biochemistry and Molecular Biology, Pennsylvania State University, 406 South Frear, University Park, PA 16802 (United States)
  2. Veterinary and Biomedical Sciences, Center for Molecular Immunology and Infectious Diseases, Pennsylvania State University, University Park, PA 16802 (United States)
  3. Department of Biochemistry and Molecular Biology, Pennsylvania State University, 406 South Frear, University Park, PA 16802 (United States), E-mail: MNT2@psu.edu
Publication Date:
OSTI Identifier:
21077989
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 368; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2007.06.017; PII: S0042-6822(07)00420-5; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; INTERFERON; KINETICS; TRANSCRIPTION; TRANSLOCATION; VIRUSES

Citation Formats

Tran, Kim C., He, Biao, and Teng, Michael N. Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5. United States: N. p., 2007. Web. doi:10.1016/j.virol.2007.06.017.
Tran, Kim C., He, Biao, & Teng, Michael N. Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5. United States. doi:10.1016/j.virol.2007.06.017.
Tran, Kim C., He, Biao, and Teng, Michael N. Sat . "Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5". United States. doi:10.1016/j.virol.2007.06.017.
@article{osti_21077989,
title = {Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5},
author = {Tran, Kim C. and He, Biao and Teng, Michael N.},
abstractNote = {Paramyxoviruses have been shown to produce proteins that inhibit interferon production and signaling. For human respiratory syncytial virus (RSV), the nonstructural NS1 and NS2 proteins have been shown to have interferon antagonist activity through an unknown mechanism. To understand further the functions of NS1 and NS2, we generated recombinant RSV in which both NS1 and NS2 were replaced by the PIV5 V protein, which has well-characterized IFN antagonist activities ({delta}NS1/2-V). Expression of V was able to partially inhibit IFN responses in {delta}NS1/2-V-infected cells. In addition, the replication kinetics of {delta}NS1/2-V were intermediate between {delta}NS1/2 and wild-type (rA2) in A549 cells. However, expression of V did not affect the ability of {delta}NS1/2-V to activate IRF3 nuclear translocation and IFN{beta} transcription. These data indicate that V was able to replace some of the IFN inhibitory functions of the RSV NS1 and NS2 proteins, but also that NS1 and NS2 have functions in viral replication beyond IFN antagonism.},
doi = {10.1016/j.virol.2007.06.017},
journal = {Virology},
number = 1,
volume = 368,
place = {United States},
year = {Sat Nov 10 00:00:00 EST 2007},
month = {Sat Nov 10 00:00:00 EST 2007}
}