Rats fed soy protein isolate (SPI) have impaired hepatic CYP1A1 induction by polycyclic aromatic hydrocarbons as a result of interference with aryl hydrocarbon receptor signaling

Singhal, Rohit; Badger, Thomas M; Ronis, Martin J

doi:10.1016/j.taap.2007.11.009

Title: Rats fed soy protein isolate (SPI) have impaired hepatic CYP1A1 induction by polycyclic aromatic hydrocarbons as a result of interference with aryl hydrocarbon receptor signaling

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Abstract

Consumption of soy diets has been found to reduce cancer incidence in animals and is associated with reduced cancer risk in humans. Previously, we have demonstrated that female Sprague-Dawley rats fed purified AIN-93G diets with soy protein isolate (SPI) as the sole protein source had reduced CYP1A1 induction and basal aryl hydrocarbon receptor (AhR) levels relative to those fed the same diet containing casein (CAS). In the present study, the molecular mechanisms underlying reduced AhR expression have been studied. The SPI-effect on AhR was not observed after feeding diets containing the purified soy isoflavones genistein or daidzein. Rat hepatoma FGC-4 cells were treated with the serum obtained from rats fed CAS- or SPI-containing diets. Reduced AhR levels (P < 0.05) were observed after 24 h exposure to SPI-serum without any changes in the overall expression of chaperone proteins-HSP90 and XAP2. SPI-serum-stimulated AhR degradation was inhibited by treating the cells with the proteasome inhibitor, MG132, and was observed to be preceded by ubiquitination of the receptor. A reduced association of XAP2 with the immunoprecipitated AhR complex was observed. SPI-serum-mediated AhR degradation was preceded by nuclear translocation of the receptor. However, the translocated receptor was found to be unable to heterodimerize withmore »« less

Authors:

Singhal, Rohit ^[1]; Badger, Thomas M ^[2]; Ronis, Martin J ^[1]

Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States)
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States)

Publication Date:: Sat Mar 01 00:00:00 EST 2008

OSTI Identifier:: 21077942

Resource Type:: Journal Article

Journal Name:: Toxicology and Applied Pharmacology

Additional Journal Information:: Journal Volume: 227; Journal Issue: 2; Other Information: DOI: 10.1016/j.taap.2007.11.009; PII: S0041-008X(07)00512-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ANTHRACENE; CASEIN; CHROMATIN; DIET; DIMETHYLBENZANTHRACENE; GLUCOCORTICOIDS; HEAT-SHOCK PROTEINS; HEPATOMAS; LIVER; POLYCYCLIC AROMATIC HYDROCARBONS; RATS; RECEPTORS; TRANSLOCATION

Citation Formats


                    Singhal, Rohit, Badger, Thomas M, Arkansas Children's Nutrition Center, Little Rock, AR 72202, Ronis, Martin J, and Arkansas Children's Nutrition Center, Little Rock, AR 72202. Rats fed soy protein isolate (SPI) have impaired hepatic CYP1A1 induction by polycyclic aromatic hydrocarbons as a result of interference with aryl hydrocarbon receptor signaling.  United States: N. p., 2008. 
        Web.  doi:10.1016/j.taap.2007.11.009.

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                    Singhal, Rohit, Badger, Thomas M, Arkansas Children's Nutrition Center, Little Rock, AR 72202, Ronis, Martin J, & Arkansas Children's Nutrition Center, Little Rock, AR 72202. Rats fed soy protein isolate (SPI) have impaired hepatic CYP1A1 induction by polycyclic aromatic hydrocarbons as a result of interference with aryl hydrocarbon receptor signaling.  United States.  https://doi.org/10.1016/j.taap.2007.11.009

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                    Singhal, Rohit, Badger, Thomas M, Arkansas Children's Nutrition Center, Little Rock, AR 72202, Ronis, Martin J, and Arkansas Children's Nutrition Center, Little Rock, AR 72202. 2008.  
        "Rats fed soy protein isolate (SPI) have impaired hepatic CYP1A1 induction by polycyclic aromatic hydrocarbons as a result of interference with aryl hydrocarbon receptor signaling".  United States.  https://doi.org/10.1016/j.taap.2007.11.009.

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@article{osti_21077942,

  title        = {Rats fed soy protein isolate (SPI) have impaired hepatic CYP1A1 induction by polycyclic aromatic hydrocarbons as a result of interference with aryl hydrocarbon receptor signaling},

  author       = {Singhal, Rohit and Badger, Thomas M and Arkansas Children's Nutrition Center, Little Rock, AR 72202 and Ronis, Martin J and Arkansas Children's Nutrition Center, Little Rock, AR 72202},

  abstractNote = {Consumption of soy diets has been found to reduce cancer incidence in animals and is associated with reduced cancer risk in humans. Previously, we have demonstrated that female Sprague-Dawley rats fed purified AIN-93G diets with soy protein isolate (SPI) as the sole protein source had reduced CYP1A1 induction and basal aryl hydrocarbon receptor (AhR) levels relative to those fed the same diet containing casein (CAS). In the present study, the molecular mechanisms underlying reduced AhR expression have been studied. The SPI-effect on AhR was not observed after feeding diets containing the purified soy isoflavones genistein or daidzein. Rat hepatoma FGC-4 cells were treated with the serum obtained from rats fed CAS- or SPI-containing diets. Reduced AhR levels (P < 0.05) were observed after 24 h exposure to SPI-serum without any changes in the overall expression of chaperone proteins-HSP90 and XAP2. SPI-serum-stimulated AhR degradation was inhibited by treating the cells with the proteasome inhibitor, MG132, and was observed to be preceded by ubiquitination of the receptor. A reduced association of XAP2 with the immunoprecipitated AhR complex was observed. SPI-serum-mediated AhR degradation was preceded by nuclear translocation of the receptor. However, the translocated receptor was found to be unable to heterodimerize with ARNT or to bind to XRE elements on the CYP1A1 enhancer. These data suggest that feeding SPI-containing diets antagonizes AhR signaling by a novel mechanism which differs from those established for known AhR antagonists.},

  doi          = {10.1016/j.taap.2007.11.009},

  url          = {https://www.osti.gov/biblio/21077942},
  journal      = {Toxicology and Applied Pharmacology},

  issn         = {0041-008X},

  number       = 2,

  volume       = 227,

  place        = {United States},

  year         = {Sat Mar 01 00:00:00 EST 2008},

  month        = {Sat Mar 01 00:00:00 EST 2008}

}

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