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Title: Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish

Abstract

The toxic effects of cadmium and other metals have been well established. A primary target of these metals is known to be the olfactory system, and fish exposed to a number of different waterborne metals display deficiencies in olfaction. Importantly, exposure over embryonic/larval development periods can cause deficits in chemosensory function in juvenile fish, but the specific cell types affected are unknown. We have previously characterized a transgenic zebrafish strain expressing the green fluorescent protein (eGFP) gene linked to the hsp70 gene promoter, and shown it to be a useful tool for examining cell-specific toxicity in living embryos and larvae. Here we show that the hsp70/eGFP transgene is strongly and specifically upregulated within the olfactory sensory neurons (OSNs) of transgenic zebrafish larvae following a brief 3-h exposure to water-borne cadmium. This molecular response was closely correlated to an endpoint for tissue damage within the olfactory placode, namely cell death. Furthermore, cadmium-induced olfactory cytotoxicity in zebrafish larvae gives rise to more permanent effects. Juvenile zebrafish briefly exposed to cadmium during early larval development display deficits in olfactory-dependent predator avoidance behaviors 4-6 weeks after a return to clean water. Lateral line neuromasts of exposed zebrafish larvae also activate both the endogenous hsp70more » gene and the hsp70/eGFP transgene. The data reveal that even a very brief exposure period that gives rise to cell death within the developing olfactory placode results in long-term deficits in olfaction, and that hsp70/eGFP may serve as an effective indicator of sublethal cadmium exposure in sensory cells.« less

Authors:
 [1];  [2];  [3];  [1];  [1];  [2];  [3];  [1];  [4]
  1. Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)
  2. (Canada)
  3. Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)
  4. (Canada), E-mail: pat.krone@usask.ca
Publication Date:
OSTI Identifier:
21077814
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 224; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2007.06.025; PII: S0041-008X(07)00281-5; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BIOLOGICAL INDICATORS; BIOLOGICAL STRESS; CADMIUM; EPITHELIUM; GENES; JUVENILES; LARVAE; NERVE CELLS; TOXICITY

Citation Formats

Blechinger, Scott R., Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Kusch, Robin C., Haugo, Kristine, Matz, Carlyn, Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Chivers, Douglas P., Krone, Patrick H., and Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan. Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish. United States: N. p., 2007. Web. doi:10.1016/j.taap.2007.06.025.
Blechinger, Scott R., Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Kusch, Robin C., Haugo, Kristine, Matz, Carlyn, Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Chivers, Douglas P., Krone, Patrick H., & Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan. Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish. United States. doi:10.1016/j.taap.2007.06.025.
Blechinger, Scott R., Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Kusch, Robin C., Haugo, Kristine, Matz, Carlyn, Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Chivers, Douglas P., Krone, Patrick H., and Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan. Mon . "Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish". United States. doi:10.1016/j.taap.2007.06.025.
@article{osti_21077814,
title = {Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish},
author = {Blechinger, Scott R. and Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan and Kusch, Robin C. and Haugo, Kristine and Matz, Carlyn and Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan and Chivers, Douglas P. and Krone, Patrick H. and Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan},
abstractNote = {The toxic effects of cadmium and other metals have been well established. A primary target of these metals is known to be the olfactory system, and fish exposed to a number of different waterborne metals display deficiencies in olfaction. Importantly, exposure over embryonic/larval development periods can cause deficits in chemosensory function in juvenile fish, but the specific cell types affected are unknown. We have previously characterized a transgenic zebrafish strain expressing the green fluorescent protein (eGFP) gene linked to the hsp70 gene promoter, and shown it to be a useful tool for examining cell-specific toxicity in living embryos and larvae. Here we show that the hsp70/eGFP transgene is strongly and specifically upregulated within the olfactory sensory neurons (OSNs) of transgenic zebrafish larvae following a brief 3-h exposure to water-borne cadmium. This molecular response was closely correlated to an endpoint for tissue damage within the olfactory placode, namely cell death. Furthermore, cadmium-induced olfactory cytotoxicity in zebrafish larvae gives rise to more permanent effects. Juvenile zebrafish briefly exposed to cadmium during early larval development display deficits in olfactory-dependent predator avoidance behaviors 4-6 weeks after a return to clean water. Lateral line neuromasts of exposed zebrafish larvae also activate both the endogenous hsp70 gene and the hsp70/eGFP transgene. The data reveal that even a very brief exposure period that gives rise to cell death within the developing olfactory placode results in long-term deficits in olfaction, and that hsp70/eGFP may serve as an effective indicator of sublethal cadmium exposure in sensory cells.},
doi = {10.1016/j.taap.2007.06.025},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 224,
place = {United States},
year = {2007},
month = {10}
}