Non-covalent associations of metal complexes with DNA duplexes by ESI-MS
- Pacific Northwest Lab., Richland, WA (United States)
- California Institute of Technology, Pasadena, CA (United States)
Transition metal complexes are being used as probes to determine the conformational structure of oligonucleotides, to recognize specific binding sites, and to probe the capability for electron transfer reactions in DNA strands. Non-covalent interactions, such as intercalation and hydrophobic groove binding of the metal complexes with oligonucleotide duplexes, provide the basis for probing DNA sequence and structure. Electrospray ionization mass spectrometry (ESI-MS) has been used for the analysis of a wide variety of biomolecules, including oligonucleotides. Recently this technique has been utilized in the characterization of non-covalent associations. In this work, the authors report the use of ESI-MS for the detection of non-covalent associations of several metal complexes with two oligonucleotide duplexes and compare these results with observations from solution.
- OSTI ID:
- 210634
- Report Number(s):
- CONF-9505261-; TRN: 96:001157-0073
- Resource Relation:
- Conference: 43. American Society of Mass Spectrometry (ASMS) conference on mass spectrometry and allied topics, Atlanta, GA (United States), 21-26 May 1995; Other Information: PBD: 1995; Related Information: Is Part Of Proceedings of the 43rd ASMS conference on mass spectrometry and allied topics; PB: 1411 p.
- Country of Publication:
- United States
- Language:
- English
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