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Title: Analysis of Sterilization Effect of Atmospheric Pressure Pulsed Plasma

Abstract

We have developed a new technology, the High Voltage Atmospheric Pressure Pulsed Plasma (HVAPPP), for bacteria killing. The aim of this paper is to present a simple device to generate plasma able to kill efficiently bacteria.

Authors:
; ;  [1]; ;  [2];  [3]
  1. Eskisehir Osmangazi University, Physics department, Eskisehir (Turkey)
  2. Eskisehir Osmangazi University Microbiology department, Eskisehir (Turkey)
  3. National Institute for Laser Plasma and Radiation Physics, Bucharest (Romania)
Publication Date:
OSTI Identifier:
21057253
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 899; Journal Issue: 1; Conference: 6. international conference of the Balkan Physical Union, Istanbul (Turkey), 22-26 Aug 2006; Other Information: DOI: 10.1063/1.2733443; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
70 PLASMA PHYSICS AND FUSION TECHNOLOGY; ATMOSPHERIC PRESSURE; BACTERIA; ELECTRIC DISCHARGES; ELECTRIC POTENTIAL; PLASMA; PLASMA PRESSURE; PLASMA PRODUCTION; STERILIZATION

Citation Formats

Ekem, N., Akan, T., Pat, S., Akgun, Y., Kiremitci, A., and Musa, G. Analysis of Sterilization Effect of Atmospheric Pressure Pulsed Plasma. United States: N. p., 2007. Web. doi:10.1063/1.2733443.
Ekem, N., Akan, T., Pat, S., Akgun, Y., Kiremitci, A., & Musa, G. Analysis of Sterilization Effect of Atmospheric Pressure Pulsed Plasma. United States. doi:10.1063/1.2733443.
Ekem, N., Akan, T., Pat, S., Akgun, Y., Kiremitci, A., and Musa, G. Mon . "Analysis of Sterilization Effect of Atmospheric Pressure Pulsed Plasma". United States. doi:10.1063/1.2733443.
@article{osti_21057253,
title = {Analysis of Sterilization Effect of Atmospheric Pressure Pulsed Plasma},
author = {Ekem, N. and Akan, T. and Pat, S. and Akgun, Y. and Kiremitci, A. and Musa, G.},
abstractNote = {We have developed a new technology, the High Voltage Atmospheric Pressure Pulsed Plasma (HVAPPP), for bacteria killing. The aim of this paper is to present a simple device to generate plasma able to kill efficiently bacteria.},
doi = {10.1063/1.2733443},
journal = {AIP Conference Proceedings},
number = 1,
volume = 899,
place = {United States},
year = {Mon Apr 23 00:00:00 EDT 2007},
month = {Mon Apr 23 00:00:00 EDT 2007}
}
  • Stable discharging of pure nitrogen can be maintained even at atmospheric pressure when alternative pulsed voltage is applied between two parallel plate electrodes. We evaluated the nitrogen plasma generated by an alternative pulsed voltage system. The excited nitrogen species in a pure nitrogen plasma was evaluated using optical emission spectroscopy. In the discharging space, the largest peak detected corresponded to the N{sub 2} second positive system. Additionally, the emission peaks from the excited nitrogen atom were detected at a pressure of as high as 45 Torr. In the downstream space, we detected emission peaks from the N{sub 2} Herman's infraredmore » system as well as the N{sub 2} second positive system. The N{sub 2} (A {sup 3}{sigma}{sub u}{sup +}) state is considered to be the origin of the N{sub 2} Herman's infrared system. The emission intensities from the N{sub 2} second positive system and the N{sub 2} Herman's infrared system increase with increasing nitrogen gas pressure, whereas the emission intensity from the N{sub 2}{sup +} first negative system decreases.« less
  • A mechanism for sterilizing Escherichia coli by a flowing postdischarge and UV radiation of argon plasma at atmospheric pressure was investigated by analyzing the surviving cells and the potassium leakage of cytoplasmic material and by morphological observation. Inactivation of E. coli results from the destruction of the cytoplasmic membrane and the outer membrane under plasma exposure and the destruction of nucleic acids by exposure to ultraviolet radiation from the plasma source.
  • Argon plasma jets penetrate deep into ambient air and create a path for oxygen radicals to sterilize microbes. A sterilization experiment with bacterial endospores indicates that an argon-oxygen plasma jet very effectively kills endospores of Bacillus atrophaeus (ATCC 9372), thereby demonstrating its capability to clean surfaces and its usefulness for reinstating contaminated equipment as free from toxic biological warfare agents. However, the spore-killing efficiency of the atmospheric-pressure argon-oxygen jet depends very sensitively on the oxygen concentration in the argon gas.
  • A nonequilibrium Ar/O{sub 2} plasma discharge at atmospheric pressure was carried out in a coaxial cylindrical reactor with a stepped electrode configuration powered by a 13.56 MHz rf power supplier. The argon glow discharge with high electron density produces oxygen reactive species in large quantities. Argon plasma jets penetrate deep into ambient air and create a path for oxygen radicals to sterilize microbes. A sterilization experiment with bacterial endospores indicates that an argon-oxygen plasma jet very effectively kills endospores of Bacillus atrophaeus (ATCC 9372), thereby demonstrating its capability to clean surfaces and its usefulness for reinstating contaminated equipment as freemore » from toxic biological warfare agents. The decimal reduction time (D values) of the Ar/O{sub 2} plasma jet at an exposure distance of 0.5-1.5 cm ranges from 5 to 57 s. An actinometric comparison of the sterilization data shows that atomic oxygen radicals play a significant role in plasma sterilization. When observed under a scanning electron microscope, the average size of the spores appears to be greatly reduced due to chemical reactions with the oxygen radicals.« less
  • Results are presented from experiments on the generation of a low-temperature nonequilibrium plasma in atmospheric-pressure heterophase gas-liquid media of different compositions: (i) a liquid with air bubbles and (ii) air with liquid aerosol. To illustrate possible application of a low-temperature plasma in a heterophase medium, experiments on the inactivation of some microorganisms by a low-temperature plasma have been performed.