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Title: Development of Protein Crystallography Beamline Using MIRRORCLE-20SX

Abstract

We are developing a laboratory-scale protein structure analysis beamline based on our portable synchrotron MIRRORCLE-20SX, which is a 20 MeV machine. Using the crystal as a target in the central orbit of the synchrotron, we will obtain parametric X-ray, the spatially resolved monochromatic X-ray. Our final goal is a development of protein crystallography basis cooperated with the protein group of the Nagahama Institute of Bio-science and Technology, which performs screening, crystallization, and protein structure analysis. The METI Kansai funded this program.

Authors:
 [1];  [2];  [3]; ;  [1];  [4]
  1. Synchrotron Light Life Science Center, Ritsumeikan Univ., 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577 (Japan)
  2. (Japan)
  3. (808) Takagaicho, minami, Omihachiman-City, Shiga, 523-0898 (Japan)
  4. Photon Production Laboratory, Ltd., 4-2-1 (808) Takagaicho, minami, Omihachiman-City, Shiga, 523-0898 (Japan)
Publication Date:
OSTI Identifier:
21056944
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 902; Journal Issue: 1; Conference: 2. international symposium on portable synchrotron light sources and advanced applications, Shiga (Japan), 15-17 Jan 2007; Other Information: DOI: 10.1063/1.2723615; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; CRYSTALLIZATION; CRYSTALLOGRAPHY; CRYSTALS; MEV RANGE; MONOCHROMATIC RADIATION; PORTABLE SOURCES; PROTEIN STRUCTURE; PROTEINS; SYNCHROTRON RADIATION; SYNCHROTRONS; X RADIATION; X-RAY DIFFRACTION

Citation Formats

Yamada, H., Faculty of science and Engineering, Ritsumeikan Univ., 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Photon Production Laboratory, Ltd., 4-2-1, Hanashima, T., Yano, Y., and Morita, M. Development of Protein Crystallography Beamline Using MIRRORCLE-20SX. United States: N. p., 2007. Web. doi:10.1063/1.2723615.
Yamada, H., Faculty of science and Engineering, Ritsumeikan Univ., 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Photon Production Laboratory, Ltd., 4-2-1, Hanashima, T., Yano, Y., & Morita, M. Development of Protein Crystallography Beamline Using MIRRORCLE-20SX. United States. doi:10.1063/1.2723615.
Yamada, H., Faculty of science and Engineering, Ritsumeikan Univ., 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Photon Production Laboratory, Ltd., 4-2-1, Hanashima, T., Yano, Y., and Morita, M. Fri . "Development of Protein Crystallography Beamline Using MIRRORCLE-20SX". United States. doi:10.1063/1.2723615.
@article{osti_21056944,
title = {Development of Protein Crystallography Beamline Using MIRRORCLE-20SX},
author = {Yamada, H. and Faculty of science and Engineering, Ritsumeikan Univ., 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577 and Photon Production Laboratory, Ltd., 4-2-1 and Hanashima, T. and Yano, Y. and Morita, M.},
abstractNote = {We are developing a laboratory-scale protein structure analysis beamline based on our portable synchrotron MIRRORCLE-20SX, which is a 20 MeV machine. Using the crystal as a target in the central orbit of the synchrotron, we will obtain parametric X-ray, the spatially resolved monochromatic X-ray. Our final goal is a development of protein crystallography basis cooperated with the protein group of the Nagahama Institute of Bio-science and Technology, which performs screening, crystallization, and protein structure analysis. The METI Kansai funded this program.},
doi = {10.1063/1.2723615},
journal = {AIP Conference Proceedings},
number = 1,
volume = 902,
place = {United States},
year = {Fri Mar 30 00:00:00 EDT 2007},
month = {Fri Mar 30 00:00:00 EDT 2007}
}
  • Beam diagnostics devices are described for the monitoring of all basic parameters relevant to protein data collection using synchrotron radiation. The parameters include the total power in the white beam, the positions and directions of the incident white and monochromatic beams, and the intensity, wavelength, and polarization in the incident monochromatic beam. Such devices have been installed on a doubly focusing wiggler beamline, BW6, at DORIS. They assure high quality in protein data collection despite fluctuations in the beam, facilitate rapid alignment of the entire beamline, and provide a basis for automatic realignment of the diffraction setup and the x-raymore » optics. {copyright} {ital 1996 American Institute of Physics.}« less
  • The technique of computed tomography (CT) has been used in various fields, such as medical, non-destructive testing (NDT), baggage checking, etc. A 3D-CT system based on the portable synchrotron 'MIRRORCLE'-series will be a novel instrument for these fields. The hard x-rays generated from the 'MIRRORCLE' have a wide energy spectrum. Light and thin materials create absorption and refraction contrast in x-ray images by the lower energy component (< 60 keV), and heavy and thick materials create absorption contrast by the higher energy component. In addition, images with higher resolutions can be obtained using 'MIRRORCLE' with a small source size ofmore » micron order. Thus, high resolution 3D-CT images of specimens containing both light and heavy materials can be obtained using 'MIRRORCLE' and a 2D-detector with a wide dynamic range. In this paper, the development and output of a 3D-CT system using the 'MIRRORCLE-6X' and a flat panel detector are reported.A 3D image of a piece of concrete was obtained. The detector was a flat panel detector (VARIAN, PAXSCAN2520) with 254 {mu}m pixel size. The object and the detector were set at 50 cm and 250 cm respectively from the x-ray source, so that the magnification was 5x. The x-ray source was a 50 {mu}m Pt rod. The rotation stage and the detector were remote-controlled using a computer, which was originally created using LabView and Visual Basic software. The exposure time was about 20 minutes. The reconstruction calculation was based on the Feldkamp algorithm, and the pixel size was 50 {mu}m. We could observe sub-mm holes and density differences in the object. Thus, the 'MIRRORCLE-CV' with 1MeV electron energy, which has same x-ray generation principles, will be an excellent x-ray source for medical diagnostics and NDT.« less
  • The protein crystallography beamline BL2S1, constructed at one of the 5 T superconducting bending-magnet ports of the Aichi synchrotron, is available to users associated with academic and industrial organizations. The beamline is mainly intended for use in X-ray diffraction measurements of single-crystals of macromolecules such as proteins and nucleic acids. Diffraction measurements for crystals of other materials are also possible, such as inorganic and organic compounds. BL2S1 covers the energy range 7–17 keV (1.8–0.7 Å) with an asymmetric-cut curved single-crystal monochromator [Ge(111) or Ge(220)], and a platinum-coated Si mirror is used for vertical focusing and as a higher-order cutoff filter.more » The beamline is equipped with a single-axis goniometer, a CCD detector, and an open-flow cryogenic sample cooler. Lastly, high-pressure protein crystallography with a diamond anvil cell can also be performed using this beamline.« less