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Title: NE-CAT Upgrade of the Bending Magnet Beamline 8BM at the ALS

Abstract

NE-CAT, North East Collaborative Access Team, bending magnet beamline (8BM) is a beamline for protein crystallography. Recently, the beamline has undergone upgrades of its x-ray optics, control system, and the addition of a robot automounter. The first crystal of the double crystal monochromator was replaced by a new design offered by Oxford Danfysik with a micro-finned, direct water-cooled crystal assembly that would provide better cooling and reduced thermal distortion, pressure induced bulge, and residual strain. Gear reduced motors were added to enhance the torque of the bender and obtain better control. For measuring displacement of the bender directly, two linear variable differential transformers (LVDT) were installed to the second crystal assembly. Early optics characterization and analysis has been carried out. Besides the upgrade of the optical components, the Blu-Ice control system originally developed at SSRL has been implemented. The installation of an automated robotic sample mounting system, from the ALS, was carried out in collaboration with the engineering group at LBNL. Preliminary results are presented.

Authors:
; ; ; ; ; ; ; ; ;  [1]
  1. NE-CAT, Advanced Photon Source, 9700 S. Cass Ave., Argonne, IL 60439 (United States)
Publication Date:
OSTI Identifier:
21052646
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436189; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; ADVANCED LIGHT SOURCE; BEAM BENDING MAGNETS; BEAM OPTICS; BEAM PRODUCTION; CONTROL SYSTEMS; COOLING; CRYSTALLOGRAPHY; CRYSTALS; DESIGN; MONOCHROMATORS; PHOTON BEAMS; PROTEINS; STRAINS; TORQUE; TRANSFORMERS; X RADIATION

Citation Formats

Wang Jun, Ogata, Craig, Yang Xiaochun, Sukumar, Narayanasami, Kourinov, Igor, Capel, Malcolm, Lynch, A. E., Rajashankar, Kanagalaghatta, Withrow, James, and Ealick, Steve. NE-CAT Upgrade of the Bending Magnet Beamline 8BM at the ALS. United States: N. p., 2007. Web. doi:10.1063/1.2436189.
Wang Jun, Ogata, Craig, Yang Xiaochun, Sukumar, Narayanasami, Kourinov, Igor, Capel, Malcolm, Lynch, A. E., Rajashankar, Kanagalaghatta, Withrow, James, & Ealick, Steve. NE-CAT Upgrade of the Bending Magnet Beamline 8BM at the ALS. United States. doi:10.1063/1.2436189.
Wang Jun, Ogata, Craig, Yang Xiaochun, Sukumar, Narayanasami, Kourinov, Igor, Capel, Malcolm, Lynch, A. E., Rajashankar, Kanagalaghatta, Withrow, James, and Ealick, Steve. Fri . "NE-CAT Upgrade of the Bending Magnet Beamline 8BM at the ALS". United States. doi:10.1063/1.2436189.
@article{osti_21052646,
title = {NE-CAT Upgrade of the Bending Magnet Beamline 8BM at the ALS},
author = {Wang Jun and Ogata, Craig and Yang Xiaochun and Sukumar, Narayanasami and Kourinov, Igor and Capel, Malcolm and Lynch, A. E. and Rajashankar, Kanagalaghatta and Withrow, James and Ealick, Steve},
abstractNote = {NE-CAT, North East Collaborative Access Team, bending magnet beamline (8BM) is a beamline for protein crystallography. Recently, the beamline has undergone upgrades of its x-ray optics, control system, and the addition of a robot automounter. The first crystal of the double crystal monochromator was replaced by a new design offered by Oxford Danfysik with a micro-finned, direct water-cooled crystal assembly that would provide better cooling and reduced thermal distortion, pressure induced bulge, and residual strain. Gear reduced motors were added to enhance the torque of the bender and obtain better control. For measuring displacement of the bender directly, two linear variable differential transformers (LVDT) were installed to the second crystal assembly. Early optics characterization and analysis has been carried out. Besides the upgrade of the optical components, the Blu-Ice control system originally developed at SSRL has been implemented. The installation of an automated robotic sample mounting system, from the ALS, was carried out in collaboration with the engineering group at LBNL. Preliminary results are presented.},
doi = {10.1063/1.2436189},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}
  • Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of amore » double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {mu}m (vertically) x 250 {mu}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5x17.5 keV, delivers 8 x 10+11 photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10-4 at 10 keV, which is suitable for MAD experiments.« less
  • The IMCA-CAT bending-magnet beamline was upgraded with a collimating mirror in order to achieve the energy resolution required to conduct high-quality multi- and single-wavelength anomalous diffraction (MAD/SAD) experiments without sacrificing beamline flux throughput. Following the upgrade, the bending-magnet beamline achieves a flux of 8 x 10{sup 11} photons s{sup -1} at 1 {angstrom} wavelength, at a beamline aperture of 1.5 mrad (horizontal) x 86 {mu}rad (vertical), with energy resolution (limited mostly by the intrinsic resolution of the monochromator optics) {delta}E/E = 1.5 x 10{sup -4} (at 10 kV). The beamline operates in a dynamic range of 7.5-17.5 keV and deliversmore » to the sample focused beam of size (FWHM) 240 {micro}m (horizontally) x 160 {micro}m (vertically). The performance of the 17-BM beamline optics and its deviation from ideally shaped optics is evaluated in the context of the requirements imposed by the needs of protein crystallography experiments. An assessment of flux losses is given in relation to the (geometric) properties of major beamline components.« less
  • Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of amore » double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {micro}m (vertically) x 250 {micro}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5 x 17.5 keV, delivers 8 x 10{sup +11} photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10{sup -4} at 10 keV, which is suitable for MAD experiments.« less
  • This report discusses: APS bending magnet source; beamline layout; beamline optical components; beamline operation; time-resolved studies station; polarization studies station; and commissioning and operational schedule.