skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments

Abstract

In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 {mu}m large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

Authors:
; ; ;  [1]; ; ; ;  [2]; ;  [3]
  1. Institute of Biophysics and X-ray Structure Research, Austrian Academy of Sciences, Schmiedlstr. 6, 8042 Graz (Austria)
  2. CNR-INFM, Lab TASC, Area di Ricerca, 34012 Basovizza (Italy)
  3. ESRF, 6 rue Jules Horowitz, BP220, 38043 Grenoble Cedex (France)
Publication Date:
OSTI Identifier:
21049260
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436299; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; EUROPEAN SYNCHROTRON RADIATION FACILITY; LAYERS; LIPOSOMES; OPTICAL SYSTEMS; PHOSPHOLIPIDS; SOLID CLUSTERS; TRAPS; X-RAY DIFFRACTION

Citation Formats

Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Burghammer, M., and Riekel, Ch. Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments. United States: N. p., 2007. Web. doi:10.1063/1.2436299.
Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Burghammer, M., & Riekel, Ch. Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments. United States. doi:10.1063/1.2436299.
Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Burghammer, M., and Riekel, Ch. Fri . "Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments". United States. doi:10.1063/1.2436299.
@article{osti_21049260,
title = {Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments},
author = {Amenitsch, H. and Rappolt, M. and Sartori, B. and Laggner, P. and Cojoc, D. and Ferrari, E. and Garbin, V. and Di Fabrizio, E. and Burghammer, M. and Riekel, Ch.},
abstractNote = {In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 {mu}m large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.},
doi = {10.1063/1.2436299},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}
  • A microscope is described that uses a {mu}m-sized ball lens, which is here termed micro-objective, manipulated with optical tweezers to image the side view of the arbitrary region of a sample. Since this micro-objective is small in size, it can go into a concave region to produce a local image of the inside which the conventional microscope cannot observe. Preliminary results show good lens performance from the micro-objective when combined with optical tweezers. {copyright} {ital 1997 American Institute of Physics.}
  • We describe an apparatus using dynamic holographic optical tweezers which is capable of trapping and aligning a single micron scale anisotropic ZnO particle for x-ray Bragg diffraction experiments. The optical tweezers demonstrate enough stability to perform coherent x-ray diffraction imaging.
  • Single-beam acoustic tweezers (SBAT), used in laboratory-on-a-chip (LOC) device has promising implications for an individual micro-particle contactless manipulation. In this study, a freestanding hydrothermal PZT thick film with excellent piezoelectric property (d{sub 33} = 270 pC/N and k{sub t} = 0.51) was employed for SBAT applications and a press-focusing technology was introduced. The obtained SBAT, acting at an operational frequency of 50 MHz, a low f-number (∼0.9), demonstrated the capability to trap and manipulate a micro-particle sized 10μm in the distilled water. These results suggest that such a device has great potential as a manipulator for a wide range ofmore » biomedical and chemical science applications.« less
  • Optical tweezers, which utilize radiation pressure to control and manipulate microscopic particles, are used for a large number of applications in biology and colloid science. In most applications a single optical tweezers is used to control one single particle. However, two or more particles can be trapped simultaneously. Although this characteristic has been used in applications, no theoretical analysis of the trapping force or the status of the trapped particles is available to our knowledge. We present our calculation, using a ray optics model, of the axial trapping forces on two rigid particles trapped in optical tweezers. The spherical aberrationmore » that results from a mismatch of the refractive indices of oil and water is also considered. The results show that the forces exerted by the optical tweezers on the two particles will cause the two particles to touch each other, and the two particles can be stably trapped at a joint equilibrium point. We also discuss the stability of axial trapping. The calculation will be useful in applications of optical tweezers to trap multiple particles.« less
  • Since their invention in 1986, optical tweezers have become a popular manipulation and force measurement tool in cellular and molecular biology. However, until recently there has not been a sophisticated model for optical tweezers on trapping cells in the ray-optics regime. We present a model for optical tweezers to calculate the optical force upon a spherically symmetric multilayer sphere representing a common biological cell. A numerical simulation of this model shows that not only is the magnitude of the optical force upon a Chinese hamster ovary cell significantly three times smaller than that upon a polystyrene bead of the samemore » size, but the distribution of the optical force upon a cell is also much different from that upon a uniform particle, and there is a 30% difference in the optical trapping stiffness of these two cases. Furthermore, under a small variant condition for the refractive indices of any adjacent layers of the sphere, this model provides a simple approximation to calculate the optical force and the stiffness of an optical tweezers system.« less