skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments

Abstract

In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 {mu}m large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

Authors:
; ; ;  [1]; ; ; ;  [2]; ;  [3]
  1. Institute of Biophysics and X-ray Structure Research, Austrian Academy of Sciences, Schmiedlstr. 6, 8042 Graz (Austria)
  2. CNR-INFM, Lab TASC, Area di Ricerca, 34012 Basovizza (Italy)
  3. ESRF, 6 rue Jules Horowitz, BP220, 38043 Grenoble Cedex (France)
Publication Date:
OSTI Identifier:
21049260
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436299; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; EUROPEAN SYNCHROTRON RADIATION FACILITY; LAYERS; LIPOSOMES; OPTICAL SYSTEMS; PHOSPHOLIPIDS; SOLID CLUSTERS; TRAPS; X-RAY DIFFRACTION

Citation Formats

Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Burghammer, M., and Riekel, Ch. Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments. United States: N. p., 2007. Web. doi:10.1063/1.2436299.
Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Burghammer, M., & Riekel, Ch. Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments. United States. doi:10.1063/1.2436299.
Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Cojoc, D., Ferrari, E., Garbin, V., Di Fabrizio, E., Burghammer, M., and Riekel, Ch. Fri . "Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments". United States. doi:10.1063/1.2436299.
@article{osti_21049260,
title = {Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments},
author = {Amenitsch, H. and Rappolt, M. and Sartori, B. and Laggner, P. and Cojoc, D. and Ferrari, E. and Garbin, V. and Di Fabrizio, E. and Burghammer, M. and Riekel, Ch.},
abstractNote = {In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 {mu}m large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.},
doi = {10.1063/1.2436299},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}