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Title: Trace Element Mapping of a Biological Specimen by a Full-Field X-ray Fluorescence Imaging Microscope with a Wolter Mirror

Abstract

A full-field X-ray fluorescence imaging microscope with a Wolter mirror was applied to the element mapping of alfalfa seeds. The X-ray fluorescence microscope was built at the Photon Factory BL3C2 (KEK). X-ray fluorescence images of several growing stages of the alfalfa seeds were obtained. X-ray fluorescence energy spectra were measured with either a solid state detector or a CCD photon counting method. The element distributions of iron and zinc which were included in the seeds were obtained using a photon counting method.

Authors:
; ; ; ; ;  [1]
  1. Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8573 (Japan)
Publication Date:
OSTI Identifier:
21049259
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436298; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ALFALFA; CHARGE-COUPLED DEVICES; DISTRIBUTION; ENERGY SPECTRA; FLUORESCENCE; IMAGES; IRON; JAPANESE ORGANIZATIONS; MAPPING; MICROSCOPY; PHOTONS; RADIATION DETECTORS; SEEDS; TRACE AMOUNTS; X RADIATION; X-RAY DETECTION; X-RAY FLUORESCENCE ANALYSIS; ZINC

Citation Formats

Hoshino, Masato, Yamada, Norimitsu, Ishino, Toyoaki, Namiki, Takashi, Watanabe, Norio, and Aoki, Sadao. Trace Element Mapping of a Biological Specimen by a Full-Field X-ray Fluorescence Imaging Microscope with a Wolter Mirror. United States: N. p., 2007. Web. doi:10.1063/1.2436298.
Hoshino, Masato, Yamada, Norimitsu, Ishino, Toyoaki, Namiki, Takashi, Watanabe, Norio, & Aoki, Sadao. Trace Element Mapping of a Biological Specimen by a Full-Field X-ray Fluorescence Imaging Microscope with a Wolter Mirror. United States. doi:10.1063/1.2436298.
Hoshino, Masato, Yamada, Norimitsu, Ishino, Toyoaki, Namiki, Takashi, Watanabe, Norio, and Aoki, Sadao. Fri . "Trace Element Mapping of a Biological Specimen by a Full-Field X-ray Fluorescence Imaging Microscope with a Wolter Mirror". United States. doi:10.1063/1.2436298.
@article{osti_21049259,
title = {Trace Element Mapping of a Biological Specimen by a Full-Field X-ray Fluorescence Imaging Microscope with a Wolter Mirror},
author = {Hoshino, Masato and Yamada, Norimitsu and Ishino, Toyoaki and Namiki, Takashi and Watanabe, Norio and Aoki, Sadao},
abstractNote = {A full-field X-ray fluorescence imaging microscope with a Wolter mirror was applied to the element mapping of alfalfa seeds. The X-ray fluorescence microscope was built at the Photon Factory BL3C2 (KEK). X-ray fluorescence images of several growing stages of the alfalfa seeds were obtained. X-ray fluorescence energy spectra were measured with either a solid state detector or a CCD photon counting method. The element distributions of iron and zinc which were included in the seeds were obtained using a photon counting method.},
doi = {10.1063/1.2436298},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}
  • A full-field x-ray fluorescence imaging microscope using a Wolter mirror was constructed at Photon Factory BL3C2. White x rays from a bending magnet were used to excite x-ray fluorescence and to enhance the x-ray fluorescence intensity. A photon-counting method using a charge-coupled device was applied to obtain an x-ray fluorescence spectrum at the image plane. The spatial distributions of some specific atoms such as Fe and Zn were obtained from photon-counting calculations. An energy resolution of 220 eV at the Fe K{alpha} line was obtained from the x-ray fluorescence spectrum by the photon-counting method. The newly developed three-dimensional element mappingsmore » of the specific atoms were accomplished by the photon-counting method and a reconstruction technique using computed tomography.« less
  • We constructed a laboratory-size three-dimensional water window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques, and observed bio-medical samples to evaluate its applicability to life science research fields. It consists of a condenser and an objective grazing incidence Wolter type I mirror, an electron-impact type oxygen Kα x-ray source, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit of around 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm scale three-dimensional fine structures were resolved.
  • The demand for an x-ray microscope has received much attention because of the desire to study living cells at a high resolution and in a hydrated environment. A Wolter type I mirror used for soft-x-ray microscope optics has many advantages. From the mirror fabrication point of view, it is necessary to perform tolerance analysis, particularly with respect to figure errors that considerably degrade the image quality.The figure tolerance of a Wolter type I mirror for a biological application in terms of the image quality and the state-of-the-art fabrication technology is discussed. The figure errors rapidly destroyed the image quality, andmore » the required slope error depended on the detector used in the soft-x-ray microscope.« less
  • We constructed a laboratory-size three-dimensional water-window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques. It consists of an electron-impact x-ray source emitting oxygen Kα x-rays, Wolter type I grazing incidence mirror optics, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit better than 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm-scale three-dimensional fine structures were resolved.
  • In the context of the inertial confinement fusion, experiments have been carried out on the Phebus laser facility to study the Rayleigh-Taylor instabilities (RTIs) at the ablation front. Premodulated brominated plastic targets (25 {mu}m thick) with a modulation wavelength between 12 and 50 {mu}m were accelerated with a temporally shaped soft x-ray pulse emitted from a hohlraum with a maximum radiation temperature of about 115 eV. The RTI growth was measured by face-on radiography using a microscope coupled with an x-ray streak camera, which has spatial and temporal resolutions of about 5 {mu}m and 50 ps, respectively. The acceleration wasmore » derived from side-on velocity measurements. The microscope we have developed is a Wolter-like microscope which consists of two toroiedal mirrors. We will present the experimental and theoretical potentialities of this microscope: characterization with an x-ray generator and plasma laser x-ray source (Phebus facility) for two-dimensional (2D) and 1D time-resolved imaging studies. Spatial resolution of about 4 {mu}m was achieved in the 1-5 keV range. The Wolter-like constitutes an interesting device for laser plasma diagnostics and will be very useful in the Laser Megajoules experiments conducted with more powerful lasers.« less