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Title: The {beta} integrins and cytoskeletal nanoimprinting

Abstract

'Nanoimprinting', whereby topographical features are directly imprinted on or in cells, has recently been documented. The mechanism(s) underlying this may explain the cause of cell behavioural alterations as a result of contact with nanotopography. Integrin-mediated cell-substrate adhesions are likely to play a key role in this phenomenon due to their involvement in bidirectional signalling between extra- and intracellular environments. We describe the effects of blocking {beta}{sub 1} and {beta}{sub 3} integrin subunits on the ability of the cytoskeleton to conform to colloidal-derived nanotopography. Scanning electron and atomic force microscopy were used to characterise substrate nanofeatures. Nanofeature circularity was calculated relative to substrate topography and the cytoskeleton of cells cultured on patterned and planar surfaces in the presence/absence of {beta}{sub 1}/{beta}{sub 3} integrin antibody. Cross-correlation investigations were similarly conducted by producing a target image relative to individual topographical nanofeatures. This was then compared with cytoskeletal patterning in the presence/absence of {beta} integrin antibodies. Inhibiting the {beta}{sub 1} subunits increased the ability of fibroblasts to nanoimprint, while inhibiting the {beta}{sub 3} subunit reduced nanoimprinting of the topography to the cell. Fibroblasts cultured on planar substrates also expressed some features sharing similarities with those observed in cells on the nanotopography, indicating an inherentmore » cytoskeletal nanopatterning at high resolution.« less

Authors:
 [1];  [2]
  1. Institute of Science and Technology in Medicine, Keele University, Thornburrow Drive, Stoke-On-Trent, ST4 7QB (United Kingdom)
  2. Center for Cell Engineering, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ (United Kingdom)
Publication Date:
OSTI Identifier:
21045944
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 314; Journal Issue: 4; Other Information: DOI: 10.1016/j.yexcr.2007.10.003; PII: S0014-4827(07)00458-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ATOMIC FORCE MICROSCOPY; ELECTRON SCANNING; FIBROBLASTS; IMAGE PROCESSING; MICROTUBULES; SUBSTRATES

Citation Formats

Wood, Mairead A., Bagnaninchi, Pierre, and Dalby, Matthew J. The {beta} integrins and cytoskeletal nanoimprinting. United States: N. p., 2008. Web. doi:10.1016/j.yexcr.2007.10.003.
Wood, Mairead A., Bagnaninchi, Pierre, & Dalby, Matthew J. The {beta} integrins and cytoskeletal nanoimprinting. United States. https://doi.org/10.1016/j.yexcr.2007.10.003
Wood, Mairead A., Bagnaninchi, Pierre, and Dalby, Matthew J. Fri . "The {beta} integrins and cytoskeletal nanoimprinting". United States. https://doi.org/10.1016/j.yexcr.2007.10.003.
@article{osti_21045944,
title = {The {beta} integrins and cytoskeletal nanoimprinting},
author = {Wood, Mairead A. and Bagnaninchi, Pierre and Dalby, Matthew J},
abstractNote = {'Nanoimprinting', whereby topographical features are directly imprinted on or in cells, has recently been documented. The mechanism(s) underlying this may explain the cause of cell behavioural alterations as a result of contact with nanotopography. Integrin-mediated cell-substrate adhesions are likely to play a key role in this phenomenon due to their involvement in bidirectional signalling between extra- and intracellular environments. We describe the effects of blocking {beta}{sub 1} and {beta}{sub 3} integrin subunits on the ability of the cytoskeleton to conform to colloidal-derived nanotopography. Scanning electron and atomic force microscopy were used to characterise substrate nanofeatures. Nanofeature circularity was calculated relative to substrate topography and the cytoskeleton of cells cultured on patterned and planar surfaces in the presence/absence of {beta}{sub 1}/{beta}{sub 3} integrin antibody. Cross-correlation investigations were similarly conducted by producing a target image relative to individual topographical nanofeatures. This was then compared with cytoskeletal patterning in the presence/absence of {beta} integrin antibodies. Inhibiting the {beta}{sub 1} subunits increased the ability of fibroblasts to nanoimprint, while inhibiting the {beta}{sub 3} subunit reduced nanoimprinting of the topography to the cell. Fibroblasts cultured on planar substrates also expressed some features sharing similarities with those observed in cells on the nanotopography, indicating an inherent cytoskeletal nanopatterning at high resolution.},
doi = {10.1016/j.yexcr.2007.10.003},
url = {https://www.osti.gov/biblio/21045944}, journal = {Experimental Cell Research},
issn = {0014-4827},
number = 4,
volume = 314,
place = {United States},
year = {2008},
month = {2}
}