Adipogenesis of bovine perimuscular preadipocytes
- Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Ag-For Centre, Edmonton, Alta., T6G 2P5 (Canada)
- Department of Animal Sciences, Washington State University, P.O. Box 646310, Pullman, WA 99164 (United States)
In this study, non-transformed progeny adipofibroblasts, derived from mature adipocyte dedifferentiation, was used as a novel in vitro model to study adipogenic gene expression in cattle. Adipofibroblasts from dedifferentiated mature perimuscular fat (PMF) tissue were cultured with differentiation stimulants until the cells exhibited morphological differentiation. Treated cells were harvested from day 2 to 16 for RNA extraction, whereas control cells were cultured without addition of stimulants. Results from time course gene expression assays by quantitative real-time PCR revealed that peroxisome proliferator-activated receptor gamma (PPAR-{gamma}), sterol regulatory element binding protein 1 (SREBP-1) and their six down-stream genes were co-expressed at day 2 post-differentiation induction. When compared to other adipogenesis culture systems, the adipogenic gene expression of bovine PMF adipofibroblasts culture was different, especially to the rodent model. Collectively, these results demonstrated PPAR-{gamma} and SREBP-1 cooperatively play a key role to regulate the re-differentiation of bovine adipofibroblasts, during early conversion stages in vitro.
- OSTI ID:
- 21043598
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 366, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2007.11.110; PII: S0006-291X(07)02487-4; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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