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Title: Hard X-ray Microscopic Imaging Of Human Breast Tissues

Abstract

X-ray microscopy with synchrotron radiation will be a useful tool for innovation of x-ray imaging in clinical and laboratory settings. It helps us observe detailed internal structure of material samples non-invasively in air. And, it also has the potential to solve some tough problems of conventional breast imaging if it could evaluate various conditions of breast tissue effectively. A new hard x-ray microscope with a spatial resolution better than 100 nm was installed at Pohang Light Source, a third generation synchrotron radiation facility in Pohang, Korea. The x-ray energy was set at 6.95 keV, and the x-ray beam was monochromatized by W/B4C monochromator. Condenser and objective zone plates were used as x-ray lenses. Zernike phase plate next to condenser zone plate was introduced for improved contrast imaging. The image of a sample was magnified 30 times by objective zone plate and 20 times by microscope objective, respectively. After additional 10 times digital magnification, the total magnifying power was up to 6000 times in the end. Phase contrast synchrotron images of 10-{mu}m-thick female breast tissue of the normal, fibroadenoma, fibrocystic change and carcinoma cases were obtained. By phase contrast imaging, hard x-rays enable us to observe many structures of breast tissuemore » without sample preparations such as staining or fixation.« less

Authors:
; ;  [1];  [2];  [3]
  1. College of Medicine, Catholic University of Daegu, 3056-6 Daemyung-Dong, Nam-Gu, Daegu 705-718 (Korea, Republic of)
  2. College of Medicine, Seoul National University, 27 Youngun-Dong, Jongro-Gu, Seoul 110-799 (Korea, Republic of)
  3. Pohang Accelerator Laboratory, Pohang University of Science and Technology, 31 San, Hyoja-dong, Pohang, KyungBuk 790-784 (Korea, Republic of)
Publication Date:
OSTI Identifier:
21043412
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436462; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOMAS; HARD X RADIATION; IMAGES; KEV RANGE; MAMMARY GLANDS; MICROSCOPY; PLATES; POHANG LIGHT SOURCE; SPATIAL RESOLUTION; SYNCHROTRON RADIATION; X-RAY DIFFRACTION; ZONES

Citation Formats

Park, Sung H., Kim, Hong T., Kim, Jong K., Jheon, Sang H., and Youn, Hwa S. Hard X-ray Microscopic Imaging Of Human Breast Tissues. United States: N. p., 2007. Web. doi:10.1063/1.2436462.
Park, Sung H., Kim, Hong T., Kim, Jong K., Jheon, Sang H., & Youn, Hwa S. Hard X-ray Microscopic Imaging Of Human Breast Tissues. United States. doi:10.1063/1.2436462.
Park, Sung H., Kim, Hong T., Kim, Jong K., Jheon, Sang H., and Youn, Hwa S. Fri . "Hard X-ray Microscopic Imaging Of Human Breast Tissues". United States. doi:10.1063/1.2436462.
@article{osti_21043412,
title = {Hard X-ray Microscopic Imaging Of Human Breast Tissues},
author = {Park, Sung H. and Kim, Hong T. and Kim, Jong K. and Jheon, Sang H. and Youn, Hwa S.},
abstractNote = {X-ray microscopy with synchrotron radiation will be a useful tool for innovation of x-ray imaging in clinical and laboratory settings. It helps us observe detailed internal structure of material samples non-invasively in air. And, it also has the potential to solve some tough problems of conventional breast imaging if it could evaluate various conditions of breast tissue effectively. A new hard x-ray microscope with a spatial resolution better than 100 nm was installed at Pohang Light Source, a third generation synchrotron radiation facility in Pohang, Korea. The x-ray energy was set at 6.95 keV, and the x-ray beam was monochromatized by W/B4C monochromator. Condenser and objective zone plates were used as x-ray lenses. Zernike phase plate next to condenser zone plate was introduced for improved contrast imaging. The image of a sample was magnified 30 times by objective zone plate and 20 times by microscope objective, respectively. After additional 10 times digital magnification, the total magnifying power was up to 6000 times in the end. Phase contrast synchrotron images of 10-{mu}m-thick female breast tissue of the normal, fibroadenoma, fibrocystic change and carcinoma cases were obtained. By phase contrast imaging, hard x-rays enable us to observe many structures of breast tissue without sample preparations such as staining or fixation.},
doi = {10.1063/1.2436462},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}
  • No abstract prepared.
  • The simultaneous post mortem visualization of soft and hard tissues using absorption-based CT remains a challenge. If the photon energy is optimized for the visualization of hard tissue, the surrounding soft tissue components are almost X-ray transparent. Therefore, the combination with other modalities such as phase-contrast CT, magnetic resonance microscopy, and histology is essential to detect the anatomical features. The combination of the 2D and 3D data sets using sophisticated segmentation and registration tools allows for conclusions about otherwise inaccessible anatomical features essential for improved patient treatments.
  • The better visualization of the human organs or internal structure is challenging to the physicist and physicians. It can lead to more understanding of the morphology, pathophysiology and the diagnosis. Conventionally used methods to investigate cells or architectures, show limited value due to sample processing procedures and lower resolution. In this respect, Zernike type phase contrast hard x-ray microscopy using 6.95keV photon energy has advantages. We investigated hair fibers of the normal healthy persons. Coherence based phase contrast images revealed three distinct structures of hair, medulla, cortex, and cuticular layer. Some different detailed characters of each sample were noted. Andmore » further details would be shown and these results would be utilized as basic data of morphologic study of human hair.« less
  • No abstract prepared.