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Title: Application of Synchrotron Radiation Imaging for Non-destructive Monitoring of Mouse Rheumatoid Arthritis Model

Abstract

This study was performed to observe microstructures of the rheumatoid arthritis induced mouse feet using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo rheumatoid arthritis induced mouse feet were obtained with an 8KeV white (unmonochromatic) beam and 20 micron thick CsI(Tl) scintillation crystal. The visual image was magnified using a x 10 microscope objective and captured using digital CCD camera. Experiments were performed at 1B2 bending magnet beamline of the Pohang Accelerator Laboratory (PAL) in Korea. Obtained images were compared with histopathologic findings from same sample. Cartilage destruction and thickened joint capsule with joint space narrowing were clearly identified at each grade of rheumatoid model with spatial resolution of as much as 1.2 micron and these findings were directly correlated with histopathologic findings. The results suggest that x-ray microscopy study of the rheumatoid arthritis model using synchrotron radiation demonstrates the potential for clinically relevant micro structure of mouse feet without sectioning and fixation.

Authors:
 [1];  [2];  [3];  [4];  [5]
  1. Department of Orthopaedic Surgery, School of Medicine, Catholic University of Deagu, 3056-6 Daemyung 4-Dong NamGu, Daegu, 705-718 (Korea, Republic of)
  2. Department of Anatomy, School of Medicine, Catholic University of Deagu, 3056-6 Daemyung 4-Dong NamGu, Daegu, 705-718 (Korea, Republic of)
  3. Internal Medicine, School of Medicine, Catholic University of Deagu, 3056-6 Daemyung 4-Dong NamGu, Daegu, 705-718 (Korea, Republic of)
  4. Radiology and Medical Engineering, School of Medicine, Catholic University of Deagu, 3056-6 Daemyung 4-Dong NamGu, Daegu, 705-718 (Korea, Republic of)
  5. Pohang Acceleratory Laboratory, Pohang University of Science and Technology, Pohang, 790-784 (Korea, Republic of)
Publication Date:
OSTI Identifier:
21043406
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436456; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CAMERAS; CARTILAGE; CHARGE-COUPLED DEVICES; COMPARATIVE EVALUATIONS; CRYSTALS; IMAGES; MICE; MICROSCOPY; MICROSTRUCTURE; MONITORING; REFRACTION; REPUBLIC OF KOREA; RHEUMATIC DISEASES; SCINTILLATIONS; SPATIAL RESOLUTION; SYNCHROTRON RADIATION; X RADIATION; X-RAY DIFFRACTION

Citation Formats

Choi, Chang-Hyuk, Kim, Hong-Tae, Choe, Jung-Yoon, Kim, Jong Ki, and Youn, Hwa Shik. Application of Synchrotron Radiation Imaging for Non-destructive Monitoring of Mouse Rheumatoid Arthritis Model. United States: N. p., 2007. Web. doi:10.1063/1.2436456.
Choi, Chang-Hyuk, Kim, Hong-Tae, Choe, Jung-Yoon, Kim, Jong Ki, & Youn, Hwa Shik. Application of Synchrotron Radiation Imaging for Non-destructive Monitoring of Mouse Rheumatoid Arthritis Model. United States. doi:10.1063/1.2436456.
Choi, Chang-Hyuk, Kim, Hong-Tae, Choe, Jung-Yoon, Kim, Jong Ki, and Youn, Hwa Shik. Fri . "Application of Synchrotron Radiation Imaging for Non-destructive Monitoring of Mouse Rheumatoid Arthritis Model". United States. doi:10.1063/1.2436456.
@article{osti_21043406,
title = {Application of Synchrotron Radiation Imaging for Non-destructive Monitoring of Mouse Rheumatoid Arthritis Model},
author = {Choi, Chang-Hyuk and Kim, Hong-Tae and Choe, Jung-Yoon and Kim, Jong Ki and Youn, Hwa Shik},
abstractNote = {This study was performed to observe microstructures of the rheumatoid arthritis induced mouse feet using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo rheumatoid arthritis induced mouse feet were obtained with an 8KeV white (unmonochromatic) beam and 20 micron thick CsI(Tl) scintillation crystal. The visual image was magnified using a x 10 microscope objective and captured using digital CCD camera. Experiments were performed at 1B2 bending magnet beamline of the Pohang Accelerator Laboratory (PAL) in Korea. Obtained images were compared with histopathologic findings from same sample. Cartilage destruction and thickened joint capsule with joint space narrowing were clearly identified at each grade of rheumatoid model with spatial resolution of as much as 1.2 micron and these findings were directly correlated with histopathologic findings. The results suggest that x-ray microscopy study of the rheumatoid arthritis model using synchrotron radiation demonstrates the potential for clinically relevant micro structure of mouse feet without sectioning and fixation.},
doi = {10.1063/1.2436456},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}