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Title: Development of Control Applications for High-Throughput Protein Crystallography Experiments

Abstract

An integrated client-server control system (PCCS) with a unified relational database (PCDB) has been developed for high-throughput protein crystallography experiments on synchrotron beamlines. The major steps in protein crystallographic experiments (purification, crystallization, crystal harvesting, data collection, and data processing) are integrated into the software. All information necessary for performing protein crystallography experiments is stored in the PCDB database (except raw X-ray diffraction data, which is stored in the Network File Server). To allow all members of a protein crystallography group to participate in experiments, the system was developed as a multi-user system with secure network access based on TCP/IP secure UNIX sockets. Secure remote access to the system is possible from any operating system with X-terminal and SSH/X11 (Secure Shell with graphical user interface) support. Currently, the system covers the high-throughput X-ray data collection stages and is being commissioned at BL5A and NW12A (PF, PF-AR, KEK, Tsukuba, Japan)

Authors:
; ; ; ; ; ; ;  [1]
  1. Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, 1-1 Oho, Tsukuba, Ibaraki (Japan)
Publication Date:
OSTI Identifier:
21043401
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436451; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; COMPUTER CODES; CONTROL; CONTROL SYSTEMS; CRYSTALLIZATION; CRYSTALLOGRAPHY; CRYSTALS; DATA PROCESSING; INTERFACES; JAPANESE ORGANIZATIONS; PROTEINS; SYNCHROTRON RADIATION; X RADIATION; X-RAY DIFFRACTION

Citation Formats

Gaponov, Yurii A., Matsugaki, Naohiro, Honda, Nobuo, Sasajima, Kumiko, Igarashi, Noriyuki, Hiraki, Masahiko, Yamada, Yusuke, and Wakatsuki, Soichi. Development of Control Applications for High-Throughput Protein Crystallography Experiments. United States: N. p., 2007. Web. doi:10.1063/1.2436451.
Gaponov, Yurii A., Matsugaki, Naohiro, Honda, Nobuo, Sasajima, Kumiko, Igarashi, Noriyuki, Hiraki, Masahiko, Yamada, Yusuke, & Wakatsuki, Soichi. Development of Control Applications for High-Throughput Protein Crystallography Experiments. United States. doi:10.1063/1.2436451.
Gaponov, Yurii A., Matsugaki, Naohiro, Honda, Nobuo, Sasajima, Kumiko, Igarashi, Noriyuki, Hiraki, Masahiko, Yamada, Yusuke, and Wakatsuki, Soichi. Fri . "Development of Control Applications for High-Throughput Protein Crystallography Experiments". United States. doi:10.1063/1.2436451.
@article{osti_21043401,
title = {Development of Control Applications for High-Throughput Protein Crystallography Experiments},
author = {Gaponov, Yurii A. and Matsugaki, Naohiro and Honda, Nobuo and Sasajima, Kumiko and Igarashi, Noriyuki and Hiraki, Masahiko and Yamada, Yusuke and Wakatsuki, Soichi},
abstractNote = {An integrated client-server control system (PCCS) with a unified relational database (PCDB) has been developed for high-throughput protein crystallography experiments on synchrotron beamlines. The major steps in protein crystallographic experiments (purification, crystallization, crystal harvesting, data collection, and data processing) are integrated into the software. All information necessary for performing protein crystallography experiments is stored in the PCDB database (except raw X-ray diffraction data, which is stored in the Network File Server). To allow all members of a protein crystallography group to participate in experiments, the system was developed as a multi-user system with secure network access based on TCP/IP secure UNIX sockets. Secure remote access to the system is possible from any operating system with X-terminal and SSH/X11 (Secure Shell with graphical user interface) support. Currently, the system covers the high-throughput X-ray data collection stages and is being commissioned at BL5A and NW12A (PF, PF-AR, KEK, Tsukuba, Japan)},
doi = {10.1063/1.2436451},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}