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Title: Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory

Abstract

We are now developing automated sample exchange robots for high-throughput protein crystallographic experiments for onsite use at synchrotron beam lines. It is part of the fully automated robotics systems being developed at the Photon Factory, for the purposes of protein crystallization, monitoring crystal growth, harvesting and freezing crystals, mounting the crystals inside a hutch and for data collection. We have already installed the sample exchange robots based on the SSRL automated mounting system at our insertion device beam lines BL-5A and AR-NW12A at the Photon Factory. In order to reduce the time required for sample exchange further, a prototype of a double-tonged system was developed. As a result of preliminary experiments with double-tonged robots, the sample exchange time was successfully reduced from 70 seconds to 10 seconds with the exception of the time required for pre-cooling and warming up the tongs.

Authors:
; ; ; ; ; ;  [1]
  1. Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801 (Japan)
Publication Date:
OSTI Identifier:
21043398
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436449; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; BIOLOGY; COOLING; CRYSTAL GROWTH; CRYSTALLIZATION; CRYSTALLOGRAPHY; CRYSTALS; MONITORING; PHOTON BEAMS; PHOTONS; PROTEINS; ROBOTS; SYNCHROTRON RADIATION; X RADIATION; X-RAY DIFFRACTION

Citation Formats

Hiraki, Masahiko, Watanabe, Shokei, Yamada, Yusuke, Matsugaki, Naohiro, Igarashi, Noriyuki, Gaponov, Yurii, and Wakatsuki, Soichi. Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory. United States: N. p., 2007. Web. doi:10.1063/1.2436449.
Hiraki, Masahiko, Watanabe, Shokei, Yamada, Yusuke, Matsugaki, Naohiro, Igarashi, Noriyuki, Gaponov, Yurii, & Wakatsuki, Soichi. Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory. United States. doi:10.1063/1.2436449.
Hiraki, Masahiko, Watanabe, Shokei, Yamada, Yusuke, Matsugaki, Naohiro, Igarashi, Noriyuki, Gaponov, Yurii, and Wakatsuki, Soichi. Fri . "Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory". United States. doi:10.1063/1.2436449.
@article{osti_21043398,
title = {Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory},
author = {Hiraki, Masahiko and Watanabe, Shokei and Yamada, Yusuke and Matsugaki, Naohiro and Igarashi, Noriyuki and Gaponov, Yurii and Wakatsuki, Soichi},
abstractNote = {We are now developing automated sample exchange robots for high-throughput protein crystallographic experiments for onsite use at synchrotron beam lines. It is part of the fully automated robotics systems being developed at the Photon Factory, for the purposes of protein crystallization, monitoring crystal growth, harvesting and freezing crystals, mounting the crystals inside a hutch and for data collection. We have already installed the sample exchange robots based on the SSRL automated mounting system at our insertion device beam lines BL-5A and AR-NW12A at the Photon Factory. In order to reduce the time required for sample exchange further, a prototype of a double-tonged system was developed. As a result of preliminary experiments with double-tonged robots, the sample exchange time was successfully reduced from 70 seconds to 10 seconds with the exception of the time required for pre-cooling and warming up the tongs.},
doi = {10.1063/1.2436449},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}