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Title: SPring-8 Structural Biology Beamlines / Current Status of Public Beamlines for Protein Crystallography at SPring-8

Abstract

SPring-8 has 2 protein crystallography beamlines for public use, BL38B1 (Structural Biology III) and BL41XU (Structural Biology I). The BL38B1 is a bending magnet beamline for routine data collection, and the BL41XU is an undulator beamline specially customized for micro beam and ultra-high resolutional experiment. The designs and the performances of each beamline are presented.

Authors:
; ; ;  [1]; ;  [2];  [1];  [3]
  1. SPring-8/JASRI, 1-1-1, Kouto, Mikazuki, Sayo-gun, Hyogo 679-5198 (Japan)
  2. RIKEN SPring-8 Center, 1-1-1, Kouto, Mikazuki, Sayo-gun, Hyogo 679-5148 (Japan)
  3. (Japan)
Publication Date:
OSTI Identifier:
21043397
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 879; Journal Issue: 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436448; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; BEAM PRODUCTION; BIOLOGY; CRYSTALLOGRAPHY; DESIGN; PERFORMANCE; PHOTON BEAMS; PROTEINS; SPRING-8 STORAGE RING; WIGGLER MAGNETS; X RADIATION; X-RAY DIFFRACTION

Citation Formats

Kawamoto, Masahide, Hasegawa, Kazuya, Shimizu, Nobutaka, Sakai, Hisanobu, Shimizu, Tetsuya, Nisawa, Atsushi, Yamamoto, Masaki, and RIKEN SPring-8 Center, 1-1-1, Kouto, Mikazuki, Sayo-gun, Hyogo 679-5148. SPring-8 Structural Biology Beamlines / Current Status of Public Beamlines for Protein Crystallography at SPring-8. United States: N. p., 2007. Web. doi:10.1063/1.2436448.
Kawamoto, Masahide, Hasegawa, Kazuya, Shimizu, Nobutaka, Sakai, Hisanobu, Shimizu, Tetsuya, Nisawa, Atsushi, Yamamoto, Masaki, & RIKEN SPring-8 Center, 1-1-1, Kouto, Mikazuki, Sayo-gun, Hyogo 679-5148. SPring-8 Structural Biology Beamlines / Current Status of Public Beamlines for Protein Crystallography at SPring-8. United States. doi:10.1063/1.2436448.
Kawamoto, Masahide, Hasegawa, Kazuya, Shimizu, Nobutaka, Sakai, Hisanobu, Shimizu, Tetsuya, Nisawa, Atsushi, Yamamoto, Masaki, and RIKEN SPring-8 Center, 1-1-1, Kouto, Mikazuki, Sayo-gun, Hyogo 679-5148. Fri . "SPring-8 Structural Biology Beamlines / Current Status of Public Beamlines for Protein Crystallography at SPring-8". United States. doi:10.1063/1.2436448.
@article{osti_21043397,
title = {SPring-8 Structural Biology Beamlines / Current Status of Public Beamlines for Protein Crystallography at SPring-8},
author = {Kawamoto, Masahide and Hasegawa, Kazuya and Shimizu, Nobutaka and Sakai, Hisanobu and Shimizu, Tetsuya and Nisawa, Atsushi and Yamamoto, Masaki and RIKEN SPring-8 Center, 1-1-1, Kouto, Mikazuki, Sayo-gun, Hyogo 679-5148},
abstractNote = {SPring-8 has 2 protein crystallography beamlines for public use, BL38B1 (Structural Biology III) and BL41XU (Structural Biology I). The BL38B1 is a bending magnet beamline for routine data collection, and the BL41XU is an undulator beamline specially customized for micro beam and ultra-high resolutional experiment. The designs and the performances of each beamline are presented.},
doi = {10.1063/1.2436448},
journal = {AIP Conference Proceedings},
number = 1,
volume = 879,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}
  • RIKEN Structural Genomics Beamlines (BL26B1 and BL26B2) at SPring-8 have been constructed for high throughput protein crystallography. The beamline operation is automated cooperating with the sample changer robot. The operation software provides a centralized control utilizing the client and server architecture. The sample management system with the networked database has been implemented to accept dry-shipped crystals from distant users.
  • Seven beamlines are operated for macromolecular crystallography (MX) at SPring-8. The three undulator beamlines are developed for cutting edge target and four bending-magnet beamlines are developed for high throughput MX. The undulator beamline, BL41XU that provides the most brilliant beam, is dedicated to obtain high quality data even from small-size and weakly-diffracting crystals. The minimum beam size at sample position is achieved to 10 {mu}m diameter using a pin-hole collimator. Its photon flux at wavelength {lambda} = 1.0 A is 2.8x10{sup 11} photons/sec. This small beam coupled with irradiation point scanning method is quite useful to take diffraction dataset frommore » small crystals by suppressing the radiation damage. These advanced technologies made a number of difficult protein structure analysis possible, (i.e. Sodium-potassium ATPase). The bending-magnet beamlines BL26B1/B2 and BL38B1 provide automatic data collection exploiting the high mobility of the beam. The beamline operation software 'BSS', sample auto-changer 'SPACE' and web-based data management software 'D-Cha' have made the automatic data collection possible. The 'Mail-in data collection system' that accepts distant users samples via courier service have made users possible to collect diffraction data without visiting SPring-8. The structural genomics research is promoted by these beamlines.« less
  • RIKEN structural genomics beamlines, BL26B1 and B2 at the SPring-8, were constructed for the structural genomics research. The main feature of the beamline is full automation of the successive data collection to maximize the beamtime efficiency. The core part of the research and development is a newly developed sample management system composed of a sample auto-changer and a database system. BL26B1 has started the user operation, and commissioning of the automatic operation with the sample management system is being progressed at BL26B2.