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Title: The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney

Abstract

Here we report the identification, cloning, and characterization of SLC10A5, which is a new member of Solute Carrier Family 10 (SLC10), also known as the 'sodium/bile acid cotransporter family'. Expression of SLC10A5/Slc10a5 was examined by quantitative real-time PCR and revealed its highest expression levels in liver and kidney in humans, rat and mouse. In rat liver and kidney, Slc10a5 expression was localized by in situ hybridization to hepatocytes and proximal tubules, respectively. A SLC10A5-FLAG fusion protein was expressed in HEK293 cells and showed an apparent molecular weight of 42 kDa after immunoprecipitation. When expressed in Xenopus laevis oocytes, the SLC10A5-FLAG protein was detected in the oocyte's plasma membrane but showed no transport activity for taurocholate, cholate, estrone-3-sulfate, or dehydroepiandrosterone sulfate. As bile acid carriers are the most related carriers to SLC10A5 though, we strongly suppose that SLC10A5 is an orphan carrier with yet non-identified substrates.

Authors:
 [1];  [1];  [1];  [2];  [2];  [1];  [3]
  1. Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, Frankfurter Street 107, 35392 Giessen (Germany)
  2. Institute of Veterinary Anatomy, Histology, and Embryology, Justus Liebig University of Giessen (Germany)
  3. Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, Frankfurter Street 107, 35392 Giessen (Germany). E-mail: Joachim.M.Geyer@vetmed.uni-giessen.de
Publication Date:
OSTI Identifier:
20991526
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 361; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2007.06.160; PII: S0006-291X(07)01349-6; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BILE ACIDS; CARRIERS; ESTRONE; HYDROXYANDROSTENONE; IN-SITU HYBRIDIZATION; LIVER; LIVER CELLS; MICE; OOCYTES; POLYMERASE CHAIN REACTION; PROTEINS; RATS; SULFATES; TUBULES

Citation Formats

Fernandes, Carla F., Godoy, Jose R., Doering, Barbara, Cavalcanti, Marcia C.O., Bergmann, Martin, Petzinger, Ernst, and Geyer, Joachim. The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.06.160.
Fernandes, Carla F., Godoy, Jose R., Doering, Barbara, Cavalcanti, Marcia C.O., Bergmann, Martin, Petzinger, Ernst, & Geyer, Joachim. The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney. United States. doi:10.1016/j.bbrc.2007.06.160.
Fernandes, Carla F., Godoy, Jose R., Doering, Barbara, Cavalcanti, Marcia C.O., Bergmann, Martin, Petzinger, Ernst, and Geyer, Joachim. Fri . "The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney". United States. doi:10.1016/j.bbrc.2007.06.160.
@article{osti_20991526,
title = {The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney},
author = {Fernandes, Carla F. and Godoy, Jose R. and Doering, Barbara and Cavalcanti, Marcia C.O. and Bergmann, Martin and Petzinger, Ernst and Geyer, Joachim},
abstractNote = {Here we report the identification, cloning, and characterization of SLC10A5, which is a new member of Solute Carrier Family 10 (SLC10), also known as the 'sodium/bile acid cotransporter family'. Expression of SLC10A5/Slc10a5 was examined by quantitative real-time PCR and revealed its highest expression levels in liver and kidney in humans, rat and mouse. In rat liver and kidney, Slc10a5 expression was localized by in situ hybridization to hepatocytes and proximal tubules, respectively. A SLC10A5-FLAG fusion protein was expressed in HEK293 cells and showed an apparent molecular weight of 42 kDa after immunoprecipitation. When expressed in Xenopus laevis oocytes, the SLC10A5-FLAG protein was detected in the oocyte's plasma membrane but showed no transport activity for taurocholate, cholate, estrone-3-sulfate, or dehydroepiandrosterone sulfate. As bile acid carriers are the most related carriers to SLC10A5 though, we strongly suppose that SLC10A5 is an orphan carrier with yet non-identified substrates.},
doi = {10.1016/j.bbrc.2007.06.160},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 361,
place = {United States},
year = {Fri Sep 14 00:00:00 EDT 2007},
month = {Fri Sep 14 00:00:00 EDT 2007}
}