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Title: Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos

Abstract

Sox7, -17 and -18 constitute the Sox subgroup F (SoxF) of HMG box transcription factor genes, which all are co-expressed in developing vascular endothelial cells in mice. Here we characterized cardiovascular phenotypes of Sox17/Sox18-double and Sox17-single null embryos during early-somite stages. Whole-mount PECAM staining demonstrated the aberrant heart looping, enlarged cardinal vein and mild defects in anterior dorsal aorta formation in Sox17 single-null embryos. The Sox17/Sox18 double-null embryos showed more severe defects in formation of anterior dorsal aorta and head/cervical microvasculature, and in some cases, aberrant differentiation of endocardial cells and defective fusion of the endocardial tube. However, the posterior dorsal aorta and allantoic microvasculature was properly formed in all of the Sox17/Sox18 double-null embryos. The anomalies in both anterior dorsal aorta and head/cervical vasculature corresponded with the weak Sox7 expression sites. This suggests the region-specific redundant activities of three SoxF members along the anteroposterior axis of embryonic vascular network.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [1];  [3];  [4];  [5];  [6]
  1. Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657 (Japan)
  2. Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611 (Japan)
  3. Department of Neurobiology and Anatomy, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132-3401 (United States)
  4. (United States)
  5. Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld 4072 (Australia)
  6. Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657 (Japan). E-mail: aykanai@mail.ecc.u-tokyo.ac.jp
Publication Date:
OSTI Identifier:
20991508
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 360; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2007.06.093; PII: S0006-291X(07)01244-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AORTA; EMBRYOS; GENES; HEAD; HEART; MICE; PHENOTYPE; TRANSCRIPTION FACTORS; VEINS

Citation Formats

Sakamoto, Youhei, Hara, Kenshiro, Kanai-Azuma, Masami, Matsui, Toshiyasu, Miura, Yutaroh, Tsunekawa, Naoki, Kurohmaru, Masamichi, Saijoh, Yukio, The Program in Human Molecular Biology and Genetics, The Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT, Koopman, Peter, and Kanai, Yoshiakira. Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.06.093.
Sakamoto, Youhei, Hara, Kenshiro, Kanai-Azuma, Masami, Matsui, Toshiyasu, Miura, Yutaroh, Tsunekawa, Naoki, Kurohmaru, Masamichi, Saijoh, Yukio, The Program in Human Molecular Biology and Genetics, The Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT, Koopman, Peter, & Kanai, Yoshiakira. Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos. United States. doi:10.1016/j.bbrc.2007.06.093.
Sakamoto, Youhei, Hara, Kenshiro, Kanai-Azuma, Masami, Matsui, Toshiyasu, Miura, Yutaroh, Tsunekawa, Naoki, Kurohmaru, Masamichi, Saijoh, Yukio, The Program in Human Molecular Biology and Genetics, The Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT, Koopman, Peter, and Kanai, Yoshiakira. Fri . "Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos". United States. doi:10.1016/j.bbrc.2007.06.093.
@article{osti_20991508,
title = {Redundant roles of Sox17 and Sox18 in early cardiovascular development of mouse embryos},
author = {Sakamoto, Youhei and Hara, Kenshiro and Kanai-Azuma, Masami and Matsui, Toshiyasu and Miura, Yutaroh and Tsunekawa, Naoki and Kurohmaru, Masamichi and Saijoh, Yukio and The Program in Human Molecular Biology and Genetics, The Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT and Koopman, Peter and Kanai, Yoshiakira},
abstractNote = {Sox7, -17 and -18 constitute the Sox subgroup F (SoxF) of HMG box transcription factor genes, which all are co-expressed in developing vascular endothelial cells in mice. Here we characterized cardiovascular phenotypes of Sox17/Sox18-double and Sox17-single null embryos during early-somite stages. Whole-mount PECAM staining demonstrated the aberrant heart looping, enlarged cardinal vein and mild defects in anterior dorsal aorta formation in Sox17 single-null embryos. The Sox17/Sox18 double-null embryos showed more severe defects in formation of anterior dorsal aorta and head/cervical microvasculature, and in some cases, aberrant differentiation of endocardial cells and defective fusion of the endocardial tube. However, the posterior dorsal aorta and allantoic microvasculature was properly formed in all of the Sox17/Sox18 double-null embryos. The anomalies in both anterior dorsal aorta and head/cervical vasculature corresponded with the weak Sox7 expression sites. This suggests the region-specific redundant activities of three SoxF members along the anteroposterior axis of embryonic vascular network.},
doi = {10.1016/j.bbrc.2007.06.093},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 360,
place = {United States},
year = {2007},
month = {8}
}