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Title: siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis

Abstract

Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1{beta} in RA synoviocytes. IL-1{beta} also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1{beta} and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA.

Authors:
 [1];  [2];  [3];  [1];  [1]; ; ; ;  [1];  [1]; ;  [4];  [3];  [3];  [1]
  1. Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan)
  2. Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan). E-mail: kawahity@koto.kpu-m.ac.jp
  3. Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto (Japan)
  4. Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan)
Publication Date:
OSTI Identifier:
20991361
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 357; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.03.190; PII: S0006-291X(07)00597-9; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; APOPTOSIS; CELL PROLIFERATION; INJURIES; MITOSIS; PATIENTS; RHEUMATIC DISEASES; SKELETON

Citation Formats

Wada, Makoto, Kawahito, Yutaka, Kimura, Shinya, Kohno, Masataka, Ishino, Hidetaka, Kimura, Mizuho, Omoto, Atsushi], Yamamoto, Aihiro, Hamaguchi, Masahide, Tsubouchi, Yasunori, Tokunaga, Daisaku, Hojo, Tatsuya, Ashihara, Eishi, Maekawa, Taira, and Yoshikawa, Toshikazu. siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.190.
Wada, Makoto, Kawahito, Yutaka, Kimura, Shinya, Kohno, Masataka, Ishino, Hidetaka, Kimura, Mizuho, Omoto, Atsushi], Yamamoto, Aihiro, Hamaguchi, Masahide, Tsubouchi, Yasunori, Tokunaga, Daisaku, Hojo, Tatsuya, Ashihara, Eishi, Maekawa, Taira, & Yoshikawa, Toshikazu. siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis. United States. doi:10.1016/j.bbrc.2007.03.190.
Wada, Makoto, Kawahito, Yutaka, Kimura, Shinya, Kohno, Masataka, Ishino, Hidetaka, Kimura, Mizuho, Omoto, Atsushi], Yamamoto, Aihiro, Hamaguchi, Masahide, Tsubouchi, Yasunori, Tokunaga, Daisaku, Hojo, Tatsuya, Ashihara, Eishi, Maekawa, Taira, and Yoshikawa, Toshikazu. Fri . "siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis". United States. doi:10.1016/j.bbrc.2007.03.190.
@article{osti_20991361,
title = {siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis},
author = {Wada, Makoto and Kawahito, Yutaka and Kimura, Shinya and Kohno, Masataka and Ishino, Hidetaka and Kimura, Mizuho and Omoto, Atsushi] and Yamamoto, Aihiro and Hamaguchi, Masahide and Tsubouchi, Yasunori and Tokunaga, Daisaku and Hojo, Tatsuya and Ashihara, Eishi and Maekawa, Taira and Yoshikawa, Toshikazu},
abstractNote = {Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1{beta} in RA synoviocytes. IL-1{beta} also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1{beta} and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA.},
doi = {10.1016/j.bbrc.2007.03.190},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 357,
place = {United States},
year = {Fri Jun 01 00:00:00 EDT 2007},
month = {Fri Jun 01 00:00:00 EDT 2007}
}