siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis

Journal Article · Fri Jun 01 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.03.190· OSTI ID:20991361

Wada, Makoto ^[1]; Kawahito, Yutaka ^[1]; Kimura, Shinya ^[2]; Kohno, Masataka ^[1]; Ishino, Hidetaka ^[1]; Kimura, Mizuho; Omoto, Atsushi; Yamamoto, Aihiro; Hamaguchi, Masahide ^[1]; Tsubouchi, Yasunori ^[1]; Tokunaga, Daisaku; Hojo, Tatsuya ^[3]; Ashihara, Eishi ^[2]; Maekawa, Taira ^[2]; Yoshikawa, Toshikazu ^[1]

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1. Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan)
2. Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto (Japan)
3. Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan)

Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1{beta} in RA synoviocytes. IL-1{beta} also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1{beta} and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA.

• OSTI ID: 20991361
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 357, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.03.190; PII: S0006-291X(07)00597-9; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
APOPTOSIS
CELL PROLIFERATION
INJURIES
MITOSIS
PATIENTS
RHEUMATIC DISEASES
SKELETON