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Title: LMO4 mRNA stability is regulated by extracellular ATP in F11 cells

Abstract

LIM only domain protein 4 (LMO4) interacts with many signaling and transcription factors to regulate cellular proliferation, differentiation and plasticity. In Drosophila, mutations in the 3' untranslated region (UTR) of the homologue dLMO cause a gain of function by increasing mRNA stability. LMO4 3'UTR contains several AU-rich elements (ARE) and is highly conserved among vertebrates, suggesting that RNA destabilizing mechanisms are evolutionarily conserved. Here, we found that extracellular ATP stabilized LMO4 mRNA in F11 cells. The LMO4 3'UTR added to a luciferase reporter markedly reduced reporter activity under basal conditions, but increased activity with ATP treatment. Two ARE motifs were characterized in the LMO4 3'UTR. ATP increased binding of HuD protein to ARE1. ARE1 conferred ATP and HuD-dependent mRNA stabilization. In contrast, sequences flanking ARE2 bound CUGBP1 and ATP destabilized this complex. Thus, our results suggest that ATP modulates recruitment of RNA-binding proteins to the 3'UTR to stabilize LMO4 mRNA.

Authors:
 [1];  [2];  [2];  [3]
  1. Ottawa Health Research Institute, Neuroscience, Centre for Stroke Recovery, 451 Smyth Road, Ottawa, Ont. K1H 8M5 (Canada). E-mail: hchen@uottawa.ca
  2. Ottawa Health Research Institute, Neuroscience, Centre for Stroke Recovery, 451 Smyth Road, Ottawa, Ont. K1H 8M5 (Canada)
  3. University of Ottawa Heart Institute, Ottawa, Ont. (Canada)
Publication Date:
OSTI Identifier:
20991353
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 357; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2007.03.113; PII: S0006-291X(07)00546-3; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ATP; CELL PROLIFERATION; DROSOPHILA; LUCIFERASE; MUTATIONS; PLASTICITY; RNA; TRANSCRIPTION FACTORS; VERTEBRATES

Citation Formats

Chen, Hsiao-Huei, Xu, Jin, Safarpour, Farzaneh, and Stewart, Alexandre F.R. LMO4 mRNA stability is regulated by extracellular ATP in F11 cells. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.113.
Chen, Hsiao-Huei, Xu, Jin, Safarpour, Farzaneh, & Stewart, Alexandre F.R. LMO4 mRNA stability is regulated by extracellular ATP in F11 cells. United States. doi:10.1016/j.bbrc.2007.03.113.
Chen, Hsiao-Huei, Xu, Jin, Safarpour, Farzaneh, and Stewart, Alexandre F.R. Fri . "LMO4 mRNA stability is regulated by extracellular ATP in F11 cells". United States. doi:10.1016/j.bbrc.2007.03.113.
@article{osti_20991353,
title = {LMO4 mRNA stability is regulated by extracellular ATP in F11 cells},
author = {Chen, Hsiao-Huei and Xu, Jin and Safarpour, Farzaneh and Stewart, Alexandre F.R.},
abstractNote = {LIM only domain protein 4 (LMO4) interacts with many signaling and transcription factors to regulate cellular proliferation, differentiation and plasticity. In Drosophila, mutations in the 3' untranslated region (UTR) of the homologue dLMO cause a gain of function by increasing mRNA stability. LMO4 3'UTR contains several AU-rich elements (ARE) and is highly conserved among vertebrates, suggesting that RNA destabilizing mechanisms are evolutionarily conserved. Here, we found that extracellular ATP stabilized LMO4 mRNA in F11 cells. The LMO4 3'UTR added to a luciferase reporter markedly reduced reporter activity under basal conditions, but increased activity with ATP treatment. Two ARE motifs were characterized in the LMO4 3'UTR. ATP increased binding of HuD protein to ARE1. ARE1 conferred ATP and HuD-dependent mRNA stabilization. In contrast, sequences flanking ARE2 bound CUGBP1 and ATP destabilized this complex. Thus, our results suggest that ATP modulates recruitment of RNA-binding proteins to the 3'UTR to stabilize LMO4 mRNA.},
doi = {10.1016/j.bbrc.2007.03.113},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 357,
place = {United States},
year = {Fri May 25 00:00:00 EDT 2007},
month = {Fri May 25 00:00:00 EDT 2007}
}