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Title: Induction of cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax edema factor as antigen delivery system

Abstract

We have investigated the efficiency of N-terminal 1-260 residues of Edema factor (EFn) as a delivery system for ESAT-6, an antigenic protein of Mycobacterium tuberculosis H{sub 37}R{sub v}, into the cytosol of mammalian cells. The EFn.ESAT-6 recombinant protein was obtained by genetic fusion of EFn and ESAT-6 DNA. Our data shows that in the presence of PA, EFn.ESAT-6 fusion protein is internalized into the cytosol of antigen presenting cells, and the splenocytes produced both Th1 and Th2 cytokines in vitro. Further, EFn.ESAT-6 elicited effective cytotoxicT lymphocyte (CTL) response in an in vitro CTL assay. This study for the first time demonstrates that EFn can be used as a vehicle to deliver heterologous proteins of therapeutic importance.

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067 (India)
  2. School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067 (India). E-mail: rakbhat01@yahoo.com
Publication Date:
OSTI Identifier:
20991352
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 357; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2007.03.166; PII: S0006-291X(07)00663-8; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; DNA; EDEMA; IN VITRO; LYMPHOCYTES; LYMPHOKINES; MYCOBACTERIUM TUBERCULOSIS

Citation Formats

Chandra, Subhash, Kaur, Manpreet, Midha, Shuchi, Gorantala, Jyotsna, and Bhatnagar, Rakesh. Induction of cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax edema factor as antigen delivery system. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.166.
Chandra, Subhash, Kaur, Manpreet, Midha, Shuchi, Gorantala, Jyotsna, & Bhatnagar, Rakesh. Induction of cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax edema factor as antigen delivery system. United States. doi:10.1016/j.bbrc.2007.03.166.
Chandra, Subhash, Kaur, Manpreet, Midha, Shuchi, Gorantala, Jyotsna, and Bhatnagar, Rakesh. Fri . "Induction of cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax edema factor as antigen delivery system". United States. doi:10.1016/j.bbrc.2007.03.166.
@article{osti_20991352,
title = {Induction of cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax edema factor as antigen delivery system},
author = {Chandra, Subhash and Kaur, Manpreet and Midha, Shuchi and Gorantala, Jyotsna and Bhatnagar, Rakesh},
abstractNote = {We have investigated the efficiency of N-terminal 1-260 residues of Edema factor (EFn) as a delivery system for ESAT-6, an antigenic protein of Mycobacterium tuberculosis H{sub 37}R{sub v}, into the cytosol of mammalian cells. The EFn.ESAT-6 recombinant protein was obtained by genetic fusion of EFn and ESAT-6 DNA. Our data shows that in the presence of PA, EFn.ESAT-6 fusion protein is internalized into the cytosol of antigen presenting cells, and the splenocytes produced both Th1 and Th2 cytokines in vitro. Further, EFn.ESAT-6 elicited effective cytotoxicT lymphocyte (CTL) response in an in vitro CTL assay. This study for the first time demonstrates that EFn can be used as a vehicle to deliver heterologous proteins of therapeutic importance.},
doi = {10.1016/j.bbrc.2007.03.166},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 357,
place = {United States},
year = {Fri May 25 00:00:00 EDT 2007},
month = {Fri May 25 00:00:00 EDT 2007}
}