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Title: Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples

Abstract

Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients.

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [3];  [4];  [4]
  1. Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States). E-mail: chuchieh.hsia@fda.hhs.gov
  2. Division of Viral Products, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States)
  3. Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States)
  4. Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States)
Publication Date:
OSTI Identifier:
20991348
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 356; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.03.087; PII: S0006-291X(07)00577-3; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; BLOOD; GLASS; HEPATITIS; MICROARRAY TECHNOLOGY; OLIGONUCLEOTIDES; PATHOGENS; PATIENTS; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; TRANSFUSIONS

Citation Formats

Hsia, Chu Chieh, Chizhikov, Vladimir E., Yang, Amy X., Selvapandiyan, Angamuthu, Hewlett, Indira, Duncan, Robert, Puri, Raj K., Nakhasi, Hira L., and Kaplan, Gerardo G. Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.087.
Hsia, Chu Chieh, Chizhikov, Vladimir E., Yang, Amy X., Selvapandiyan, Angamuthu, Hewlett, Indira, Duncan, Robert, Puri, Raj K., Nakhasi, Hira L., & Kaplan, Gerardo G. Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples. United States. doi:10.1016/j.bbrc.2007.03.087.
Hsia, Chu Chieh, Chizhikov, Vladimir E., Yang, Amy X., Selvapandiyan, Angamuthu, Hewlett, Indira, Duncan, Robert, Puri, Raj K., Nakhasi, Hira L., and Kaplan, Gerardo G. Fri . "Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples". United States. doi:10.1016/j.bbrc.2007.03.087.
@article{osti_20991348,
title = {Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples},
author = {Hsia, Chu Chieh and Chizhikov, Vladimir E. and Yang, Amy X. and Selvapandiyan, Angamuthu and Hewlett, Indira and Duncan, Robert and Puri, Raj K. and Nakhasi, Hira L. and Kaplan, Gerardo G.},
abstractNote = {Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients.},
doi = {10.1016/j.bbrc.2007.03.087},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 356,
place = {United States},
year = {Fri May 18 00:00:00 EDT 2007},
month = {Fri May 18 00:00:00 EDT 2007}
}