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Title: Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma

Abstract

Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of S100, has been identified as a component of a novel ubiquitinylation complex leading to {beta}-catenin degradation, which was found to be related to the malignant phenotypes of gastric cancer. However, the roles of CacyBP/SIP in renal cell carcinoma still remain unclear. In the present study, we had analyzed the expression of the CacyBP/SIP protein in human renal cancer cells and clinical tissue samples. The possible roles of CacyBP/SIP in regulating the malignant phenotype of renal cancer cells were also investigated. The results demonstrated that the expression of CacyBP/SIP was markedly down-regulated in renal cell carcinoma tissues and cell lines. Ectopic overexpression of CacyBP/SIP in A498 cells inhibited the proliferation of this cell and delayed cell cycle progression significantly, which might be related to the down-regulation of Cyclin D1 through reducing {beta}-catenin protein. CacyBP/SIP also suppressed colony formation in soft agar and its tumorigenicity in nude mice. Taken together, our work showed that CacyBP/SIP, as a novel down-regulated gene in renal cell carcinoma, suppressed proliferation and tumorigenesis of renal cancer cells.

Authors:
 [1];  [2];  [1];  [2];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [3]
  1. State Key Laboratory of Cancer Biology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi (China)
  2. (China)
  3. State Key Laboratory of Cancer Biology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi (China). E-mail: fandaim@fmmu.edu.cn
Publication Date:
OSTI Identifier:
20991341
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 356; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.03.080; PII: S0006-291X(07)00525-6; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AGAR; CARCINOMAS; CELL CYCLE; CELL PROLIFERATION; COLONY FORMATION; GENE REGULATION; GROWTH; INHIBITION; KIDNEYS; MICE; PHENOTYPE; PROTEINS

Citation Formats

Sun, Shiren, Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, Ning, Xiaoxuan, Department of Geriatrics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, Liu, Jie, Liu, Lili, Chen, Yu, Han, Shuang, Zhang, Yanqi, Liang, Jie, Wu, Kaichun, and Fan, Daiming. Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.080.
Sun, Shiren, Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, Ning, Xiaoxuan, Department of Geriatrics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, Liu, Jie, Liu, Lili, Chen, Yu, Han, Shuang, Zhang, Yanqi, Liang, Jie, Wu, Kaichun, & Fan, Daiming. Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma. United States. doi:10.1016/j.bbrc.2007.03.080.
Sun, Shiren, Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, Ning, Xiaoxuan, Department of Geriatrics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, Liu, Jie, Liu, Lili, Chen, Yu, Han, Shuang, Zhang, Yanqi, Liang, Jie, Wu, Kaichun, and Fan, Daiming. Fri . "Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma". United States. doi:10.1016/j.bbrc.2007.03.080.
@article{osti_20991341,
title = {Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma},
author = {Sun, Shiren and Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi and Ning, Xiaoxuan and Department of Geriatrics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi and Liu, Jie and Liu, Lili and Chen, Yu and Han, Shuang and Zhang, Yanqi and Liang, Jie and Wu, Kaichun and Fan, Daiming},
abstractNote = {Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of S100, has been identified as a component of a novel ubiquitinylation complex leading to {beta}-catenin degradation, which was found to be related to the malignant phenotypes of gastric cancer. However, the roles of CacyBP/SIP in renal cell carcinoma still remain unclear. In the present study, we had analyzed the expression of the CacyBP/SIP protein in human renal cancer cells and clinical tissue samples. The possible roles of CacyBP/SIP in regulating the malignant phenotype of renal cancer cells were also investigated. The results demonstrated that the expression of CacyBP/SIP was markedly down-regulated in renal cell carcinoma tissues and cell lines. Ectopic overexpression of CacyBP/SIP in A498 cells inhibited the proliferation of this cell and delayed cell cycle progression significantly, which might be related to the down-regulation of Cyclin D1 through reducing {beta}-catenin protein. CacyBP/SIP also suppressed colony formation in soft agar and its tumorigenicity in nude mice. Taken together, our work showed that CacyBP/SIP, as a novel down-regulated gene in renal cell carcinoma, suppressed proliferation and tumorigenesis of renal cancer cells.},
doi = {10.1016/j.bbrc.2007.03.080},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 356,
place = {United States},
year = {Fri May 18 00:00:00 EDT 2007},
month = {Fri May 18 00:00:00 EDT 2007}
}