skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells

Abstract

Mesenchymal stem cells (MSCs) are attractive candidates for cell based therapies. However, the mechanisms responsible for stem cell migration and homing after transplantation remain unknown. It has been shown that insulin-like growth factor-1 (IGF-1) induces proliferation and migration of some cell types, but its effects on stem cells have not been investigated. We isolated and cultured MSC from rat bone marrow, and found that IGF-1 increased the expression levels of the chemokine receptor CXCR4 (receptor for stromal cell-derived factor-1, SDF-1). Moreover, IGF-1 markedly increased the migratory response of MSC to SDF-1. The IGF-1-induced increase in MSC migration in response to SDF-1 was attenuated by PI3 kinase inhibitor (LY294002 and wortmannin) but not by mitogen-activated protein/ERK kinase inhibitor PD98059. Our data indicate that IGF-1 increases MSC migratory responses via CXCR4 chemokine receptor signaling which is PI3/Akt dependent. These findings provide a new paradigm for biological effects of IGF-1 on MSC and have implications for the development of novel stem cell therapeutic strategies.

Authors:
 [1];  [2];  [3];  [3];  [4];  [5]
  1. Laboratory of Heart Failure and Stem Cell, Texas Heart Institute, Houston, TX 77030 (United States). E-mail: Yangxin_li@yahoo.com
  2. Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Provincial Cardiovascular Institute, Guanzhou, Guandong 510080 (China). E-mail: yuxycn@hotmail.com
  3. Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Provincial Cardiovascular Institute, Guanzhou, Guandong 510080 (China)
  4. Section of Cardiology, Xiangya Hospital of Central-South University, Changsha, Hunan 410008 (China)
  5. Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030 (United States)
Publication Date:
OSTI Identifier:
20991338
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 356; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2007.03.049; PII: S0006-291X(07)00523-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL EFFECTS; BONE MARROW; CELL PROLIFERATION; GROWTH FACTORS; INSULIN; RATS; RECEPTORS; STEM CELLS; THERAPY

Citation Formats

Li, Yangxin, Yu, XiYong, Lin, ShuGuang, Li, XiaoHong, Zhang, Saidan, and Song, Yao-Hua. Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.049.
Li, Yangxin, Yu, XiYong, Lin, ShuGuang, Li, XiaoHong, Zhang, Saidan, & Song, Yao-Hua. Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells. United States. doi:10.1016/j.bbrc.2007.03.049.
Li, Yangxin, Yu, XiYong, Lin, ShuGuang, Li, XiaoHong, Zhang, Saidan, and Song, Yao-Hua. Fri . "Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells". United States. doi:10.1016/j.bbrc.2007.03.049.
@article{osti_20991338,
title = {Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells},
author = {Li, Yangxin and Yu, XiYong and Lin, ShuGuang and Li, XiaoHong and Zhang, Saidan and Song, Yao-Hua},
abstractNote = {Mesenchymal stem cells (MSCs) are attractive candidates for cell based therapies. However, the mechanisms responsible for stem cell migration and homing after transplantation remain unknown. It has been shown that insulin-like growth factor-1 (IGF-1) induces proliferation and migration of some cell types, but its effects on stem cells have not been investigated. We isolated and cultured MSC from rat bone marrow, and found that IGF-1 increased the expression levels of the chemokine receptor CXCR4 (receptor for stromal cell-derived factor-1, SDF-1). Moreover, IGF-1 markedly increased the migratory response of MSC to SDF-1. The IGF-1-induced increase in MSC migration in response to SDF-1 was attenuated by PI3 kinase inhibitor (LY294002 and wortmannin) but not by mitogen-activated protein/ERK kinase inhibitor PD98059. Our data indicate that IGF-1 increases MSC migratory responses via CXCR4 chemokine receptor signaling which is PI3/Akt dependent. These findings provide a new paradigm for biological effects of IGF-1 on MSC and have implications for the development of novel stem cell therapeutic strategies.},
doi = {10.1016/j.bbrc.2007.03.049},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 356,
place = {United States},
year = {Fri May 11 00:00:00 EDT 2007},
month = {Fri May 11 00:00:00 EDT 2007}
}