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Title: Truncated pStat5B is associated with the Idd4 locus in NOD mice

Abstract

We investigate JAK-STAT5 activation and its relationship to full-length Stat5B (FL-Stat5) and constitutive phosphorylated carboxy-truncated Stat5B (ct-pStat5) in four different strains of mouse. Our electrophoresis mobility shift assays data indicate constitutive phosphorylation of full-length-Stat5 (p < 0.001) and DNA binding in NOD but not in B6 mice. Our data suggest that the relative ratio of FL-Stat5: ct-Stat5 in NOD is 5- to 8-fold lower (p < 0.0001) when compared with normal B6 mice. Additionally, EMSAs data from B6.NOD/c11 suggest contribution of Idd4 susceptibility locus on chromosome 11 in constitutive phosphorylation of Stat5 in NOD mice. The presence of ct-pStat5 in regulatory T cells of NOD mice suggests this form of Stat5 is associated with impaired function of Tregs in NOD mouse. In agreement with our previous report the JAK-Stat5B defective pathway in NOD mice along with other defective factors is associated with the pathogenesis of autoimmune type 1 diabetes in NOD mice.

Authors:
 [1];  [2];  [3];  [3]
  1. Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL (United States). E-mail: semiromi@pathology.ufl.edu
  2. Departments of Microbiology and Internal Medicine, University of Virginia School of Medicine, Charlottesville, VA (United States)
  3. Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL (United States)
Publication Date:
OSTI Identifier:
20991335
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 356; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2007.03.028; PII: S0006-291X(07)00488-3; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHROMOSOMES; DIABETES MELLITUS; DNA; ELECTROPHORESIS; MICE; NITRIC OXIDE; PATHOGENESIS; PHOSPHORYLATION

Citation Formats

Davoodi-Semiromi, Abdoreza, McDuffie, Marcia, Litherland, Sally, and Clare-Salzler, Michael. Truncated pStat5B is associated with the Idd4 locus in NOD mice. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.028.
Davoodi-Semiromi, Abdoreza, McDuffie, Marcia, Litherland, Sally, & Clare-Salzler, Michael. Truncated pStat5B is associated with the Idd4 locus in NOD mice. United States. doi:10.1016/j.bbrc.2007.03.028.
Davoodi-Semiromi, Abdoreza, McDuffie, Marcia, Litherland, Sally, and Clare-Salzler, Michael. Fri . "Truncated pStat5B is associated with the Idd4 locus in NOD mice". United States. doi:10.1016/j.bbrc.2007.03.028.
@article{osti_20991335,
title = {Truncated pStat5B is associated with the Idd4 locus in NOD mice},
author = {Davoodi-Semiromi, Abdoreza and McDuffie, Marcia and Litherland, Sally and Clare-Salzler, Michael},
abstractNote = {We investigate JAK-STAT5 activation and its relationship to full-length Stat5B (FL-Stat5) and constitutive phosphorylated carboxy-truncated Stat5B (ct-pStat5) in four different strains of mouse. Our electrophoresis mobility shift assays data indicate constitutive phosphorylation of full-length-Stat5 (p < 0.001) and DNA binding in NOD but not in B6 mice. Our data suggest that the relative ratio of FL-Stat5: ct-Stat5 in NOD is 5- to 8-fold lower (p < 0.0001) when compared with normal B6 mice. Additionally, EMSAs data from B6.NOD/c11 suggest contribution of Idd4 susceptibility locus on chromosome 11 in constitutive phosphorylation of Stat5 in NOD mice. The presence of ct-pStat5 in regulatory T cells of NOD mice suggests this form of Stat5 is associated with impaired function of Tregs in NOD mouse. In agreement with our previous report the JAK-Stat5B defective pathway in NOD mice along with other defective factors is associated with the pathogenesis of autoimmune type 1 diabetes in NOD mice.},
doi = {10.1016/j.bbrc.2007.03.028},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 356,
place = {United States},
year = {Fri May 11 00:00:00 EDT 2007},
month = {Fri May 11 00:00:00 EDT 2007}
}