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Title: Asynchrony in the growth and motility responses to environmental changes by individual bacterial cells

Abstract

Knowing how individual cells respond to environmental changes helps one understand phenotypic diversity in a bacterial cell population, so we simultaneously monitored the growth and motility of isolated motile Escherichia coli cells over several generations by using a method called on-chip single-cell cultivation. Starved cells quickly stopped growing but remained motile for several hours before gradually becoming immotile. When nutrients were restored the cells soon resumed their growth and proliferation but remained immotile for up to six generations. A flagella visualization assay suggested that deflagellation underlies the observed loss of motility. This set of results demonstrates that single-cell transgenerational study under well-characterized environmental conditions can provide information that will help us understand distinct functions within individual cells.

Authors:
 [1];  [2];  [1];  [3]
  1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo (Japan)
  2. Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (Japan)
  3. Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (Japan). E-mail: yasuda.bmi@tmd.ac.jp
Publication Date:
OSTI Identifier:
20991325
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 356; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.03.001; PII: S0006-291X(07)00462-7; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL CULTURES; CELL PROLIFERATION; CULTIVATION; ESCHERICHIA COLI; GROWTH; NUTRIENTS

Citation Formats

Umehara, Senkei, Hattori, Akihiro, Inoue, Ippei, and Yasuda, Kenji. Asynchrony in the growth and motility responses to environmental changes by individual bacterial cells. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.03.001.
Umehara, Senkei, Hattori, Akihiro, Inoue, Ippei, & Yasuda, Kenji. Asynchrony in the growth and motility responses to environmental changes by individual bacterial cells. United States. doi:10.1016/j.bbrc.2007.03.001.
Umehara, Senkei, Hattori, Akihiro, Inoue, Ippei, and Yasuda, Kenji. Fri . "Asynchrony in the growth and motility responses to environmental changes by individual bacterial cells". United States. doi:10.1016/j.bbrc.2007.03.001.
@article{osti_20991325,
title = {Asynchrony in the growth and motility responses to environmental changes by individual bacterial cells},
author = {Umehara, Senkei and Hattori, Akihiro and Inoue, Ippei and Yasuda, Kenji},
abstractNote = {Knowing how individual cells respond to environmental changes helps one understand phenotypic diversity in a bacterial cell population, so we simultaneously monitored the growth and motility of isolated motile Escherichia coli cells over several generations by using a method called on-chip single-cell cultivation. Starved cells quickly stopped growing but remained motile for several hours before gradually becoming immotile. When nutrients were restored the cells soon resumed their growth and proliferation but remained immotile for up to six generations. A flagella visualization assay suggested that deflagellation underlies the observed loss of motility. This set of results demonstrates that single-cell transgenerational study under well-characterized environmental conditions can provide information that will help us understand distinct functions within individual cells.},
doi = {10.1016/j.bbrc.2007.03.001},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 356,
place = {United States},
year = {Fri May 04 00:00:00 EDT 2007},
month = {Fri May 04 00:00:00 EDT 2007}
}