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Title: SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron

Abstract

Special AT-rich binding protein 1 (SATB1), a cell type-specific nuclear matrix attachment region (MAR) DNA-binding protein, tethers to a specific DNA sequence and regulates gene expression through chromatin remodeling and HDAC (histone deacetylase complex) recruitment. In this study, a SATB1 eukaryotic expression plasmid was transfected into the human erythroleukemia K562 cell line and individual clones that stably over-expressed the SATB1 protein were isolated. Microarray analysis revealed that hundreds of genes were either up- or down-regulated in the SATB1 over-expressing K562 cell lines. One of these was the extra-cellular matrix glycoprotein, SPARC (human secreted protein acidic and rich in cysteine). siRNA knock-down of SATB1 also reduced SPARC expression, which was consistent with elevated SPARC levels in the SATB1 over-expressing cell line. Bioinformatics software Mat-inspector showed that a 17 bp DNA sequence in the third intron of SPARC possessed a high potential for SATB1 binding; a finding confirmed by Chromatin immunoprecipitation (ChIP) with anti-SATB1 antibody. Our results show for the first time that forced-expression of SATB1 in K562 cells triggers SPARC up-regulation by binding to a 17 bp DNA sequence in the third intron.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Biochemistry and Molecular Biology, Medical School of Shanghai Jiao Tong University, 280 Chongqing Road(S), Shanghai 200025 (China)
  2. Department of Biochemistry and Molecular Biology, Medical School of Shanghai Jiao Tong University, 280 Chongqing Road(S), Shanghai 200025 (China). E-mail: jianlu@shsmu.edu.cn
Publication Date:
OSTI Identifier:
20991311
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 356; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2007.01.201; PII: S0006-291X(07)00017-4; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; CHROMATIN; COMPLEXES; COMPUTER CODES; CYSTEINE; DNA; DNA SEQUENCING; GENE REGULATION; GLYCOPROTEINS; NUCLEAR MATRIX

Citation Formats

Li, K., Cai, R., Dai, B.B., Zhang, X.Q., Wang, H.J., Ge, S.F., Xu, W.R., and Lu, J.. SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.01.201.
Li, K., Cai, R., Dai, B.B., Zhang, X.Q., Wang, H.J., Ge, S.F., Xu, W.R., & Lu, J.. SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron. United States. doi:10.1016/j.bbrc.2007.01.201.
Li, K., Cai, R., Dai, B.B., Zhang, X.Q., Wang, H.J., Ge, S.F., Xu, W.R., and Lu, J.. Fri . "SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron". United States. doi:10.1016/j.bbrc.2007.01.201.
@article{osti_20991311,
title = {SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron},
author = {Li, K. and Cai, R. and Dai, B.B. and Zhang, X.Q. and Wang, H.J. and Ge, S.F. and Xu, W.R. and Lu, J.},
abstractNote = {Special AT-rich binding protein 1 (SATB1), a cell type-specific nuclear matrix attachment region (MAR) DNA-binding protein, tethers to a specific DNA sequence and regulates gene expression through chromatin remodeling and HDAC (histone deacetylase complex) recruitment. In this study, a SATB1 eukaryotic expression plasmid was transfected into the human erythroleukemia K562 cell line and individual clones that stably over-expressed the SATB1 protein were isolated. Microarray analysis revealed that hundreds of genes were either up- or down-regulated in the SATB1 over-expressing K562 cell lines. One of these was the extra-cellular matrix glycoprotein, SPARC (human secreted protein acidic and rich in cysteine). siRNA knock-down of SATB1 also reduced SPARC expression, which was consistent with elevated SPARC levels in the SATB1 over-expressing cell line. Bioinformatics software Mat-inspector showed that a 17 bp DNA sequence in the third intron of SPARC possessed a high potential for SATB1 binding; a finding confirmed by Chromatin immunoprecipitation (ChIP) with anti-SATB1 antibody. Our results show for the first time that forced-expression of SATB1 in K562 cells triggers SPARC up-regulation by binding to a 17 bp DNA sequence in the third intron.},
doi = {10.1016/j.bbrc.2007.01.201},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 356,
place = {United States},
year = {Fri Apr 27 00:00:00 EDT 2007},
month = {Fri Apr 27 00:00:00 EDT 2007}
}